Week 6

Question 1

What does tube IAT consist of

Answer 1

-detects antibodies (cold and Warm)
-Read at IS, 37, and AHG
-Crossmatching (Full IAT) Prewarm Technique IAT if cold antibody is present

Question 2

What does MTS consist of

Answer 2

-detects Warm IgG antibodies
-detect very strong Cold antibody (MF)
-Full Crossmatch (IAT)

Question 3

if only one screening cell is positive does that mean there is only one AB

Answer 3

it just means the AG (on RBC)to all AB can be on same cell

Question 4

Positive Antibody Screen
must have

Answer 4

-ID all AB detected regardless of significance because one AB can mask the other.
-serum/plasma tested against PANEL CELLS (unknown with known phenotype)
-must have an auto control cell where we test the pt cells with their own plasma

Question 5

What should panel cells have

many positive cells, few, variation, auto control negative, auto control positive

Answer 5

minimum of 10 cells (so you can ID a pattern of homo/hetero cells of all significant AB)

many positive- multiple AB or AB to high incidence AG

few positive - AB to low incidence AG or weak AB showing dosage

Variation in strength- AB showing dosage

Autocontrol is neg- Allo AB

Auto control is pos - Allo AB or Pt AB has been sensitized

Question 6

What are allo antibodies

Answer 6

exposed through transfusion or pregnangy
-red cell iummeW

Question 7

What are auto antibodies

Answer 7

2ndry to infectious diseases
-primary idiopathic autoimmune
-non specific and can react with all cells in panel

Question 8

when can phenotyping be done

Answer 8

if the patient has not been transfused within the last 3 months

Question 9

When can elution testing be done

Answer 9

if the patient has been transfused in the last 3 months (part of AB ID)

Question 10

When doing an investigation what should you know about AB

Answer 10

-which are destroyed/enhanced
-IgM or IgG
-favored phase reaction
-high or low incidence

Question 11

What should you look for eleminating

Answer 11

• look at non reacting cells
  “reverse elimination logic”
  -if strong AG (homo expression) did not react with pt plasma then the AB to that AG is not present
  -0 reactions are negative

Question 12

Anti-D, -𝐋𝐞𝐚 and -𝐋𝐞𝐛

Answer 12

One non-reacting Positive cell

Question 13

Anti-C, -E, -c, -e, -K, -k, -Fya, -Fyb, -Jka, -Jkb, -M, -N, -S, -s

Answer 13

One non-reacting Homozygous cell

Anti-K can be ruled out with 2 heterozygous cells if there are no homozygous cells present on panels

Question 14

Anti -C and -E

Answer 14

Two non reacting heterozygous cells in the presence of Anti-D

One non reacting heterozygous cell if RhIg was Transfused

Question 15

Anti- P1

Answer 15

Two non reacting P1+ cells

Question 16

you dont eliminate on a heterozygous BUT

Answer 16

if hetero JKa JK (a+b+) did not react then Anti Jka or Anti Jkb are less likely to be present in pt plasma

-since it can show dosage it is not eliminated and listed under least likely of AB