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Physiology 1 Lab > Week 3-6 Blood > Flashcards

Week 3-6 Blood Flashcards

1
Q

Blood group antigen

A

On putter surface of erythrocytes membrane
Important for transfusion

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2
Q

Antibody

A

-present in serum
-natural antibodies-> anti-A, antiB appear 2-8months after birth
-immune antibody -> anti D ( present only, when organism is already met with the antigen
-IGM cannot cross placenta

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3
Q

Bleeding time material

A

*antiseptic solution
*hypodermic needle
*tissue
*stop watch

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4
Q

Bleeding time procedure

A
  • clean-puncture- blot the blood -no bleeding stop the watch
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5
Q

Bleeding time evaluation
normal and patho

A

Normal:2-3mins
Patho:more than 5mins

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6
Q

Clotting time material

A

Same as bleeding time
Watch glass
Glass rod
Water bath 37 degree

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7
Q

Clotting time procedure

A

*4-5drops of blood on watch glass
*waterbath
*glassrod every 20 sec
* stop the watch when 1st fibrin fivér appear

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8
Q

Evolution of clotting time

A

Normal:5-10 mins

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9
Q

Prothrombin time

A

-the time needed prothrombin-thrombin -fibrin time

-extrinsic pathway Bitamin k and ca2+

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10
Q

Procedure of prothrombin time

A
  • place watchglass and reagent thromboplastin-Ca2+ into water bath
  • 0.1 ml citrated plasma onto watch glass
    -0.2 ml reagent into plasma sample
    -dip the glass rod 2 times/sec
    Normal: 13-22sec
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11
Q

Prothrombin index(quick index)

A

Prothrombin index (%)= (prothrombin time of standard plasma (sec)/prothrombin time of sample(sec))*100
Normal range 70-120%

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12
Q

International Normalized Ratio

A

INR=(PTsample/PTstandard)squareISI
Normal range 0.8- 1.2

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13
Q

Higher INR, lower Quick index

A

Risk of Bleeding

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14
Q

Lower INR higher Quick index

A

Risk of thrombosis

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15
Q

Hematocrit mean

A

The ratio of volume of fundaments to the volume of the whole blood

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16
Q

Hemotocrit material

A

Heparin coated capillary
Plasticine
Centrifuges
Antiseptic
Needle
Blood

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17
Q

Hematocrit procedure

A

Blood-filled the capillary-seal with plasticine-centrifuge 10-20000RPM for 3-10mins

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18
Q

Evaluation of the hematocrit result

A

Normal: male:42-52%
Female: 37-47%

19
Q

Red blood cell counting material

A

Mixing pipette(1:100)
Hayek solution: hypertonic salt solution prevents RBC stick together
Burker’s chamber
Coverslip
Microscope
Antiseptic
Needle
Blood

20
Q

Red blood cell counting procedure

A

Burker’s chamber with coverslip
Using 1/201/201/10 small square
Blood1+100 hayem’s solution mix it, first 2 drops nope and to the slide and then using 40* objects by using l law

21
Q

Red blood cell counting caculation

A

RBC Count= (average No. of RBCs per area)* dilution(100)*1/1/4000

22
Q

Normal range RBC counting

A

Male:4.5-6 million/ul

Female: 3.8-5.2 million/ul

23
Q

Mean size of RBC material

A

Isotonic solution
Microscope glass slide
Coverslip
Microscope + eyepiece graticule
Immersion oil
Antiseptic
Needle

24
Q

Mean size of RBC procedure

A

Immersion oil lens 100* calibration eyepiece graticule to the smallest unit of the scale of a calibration slide (10um) count 200-300 RBC

25
Normal RBC size
7-8um , 2-2.5thickness
26
price jones curves right and left shift
Right:macrocytosis Left: microcytosis
27
Erythrocytes sedimentation rate is
The rate at which RBCs settle down in a tube during 1 hr
28
The higher the ration of globulin and fibrinogen to albumin how is the ESR will be?
Increase ESR
29
Normal range of ESR
Male:2-6mm/hr Female: 3-10 mm/hr
30
The dye of Reticulocyte counting
Supravital: Brilliant cresyl blue stain
31
Normal range of Reticulocyte counting
0.7-1.5%
32
Dye for palate counting
Ress Ecker *10 dilution
33
Normal range of thrombocyte for adult
150,000-400,000/ul
34
Dye for leukocytes counting
Turk solution *10 dilution
35
Normal range of WBC counting
4000-10000/ul
36
Differential leukocytes count method
Dry blood slide to solution 1 May-grunwald for 3 mins then 2nd solution with May-Grunwald and deionised water 1:1 for 3 mins then shoultion 3, 10:1 deionnised water and Giemsa solution 10-15 mins
37
Normal osmotic range is
0.42-0.46% NaCl solition
38
Complete hemolysis
0.30-0.34% NaCls solution
39
Determination of Hb concentration by the Drabkin’s method
Transfrom Hb to Meth-Hb, ferrous(Fe2+)to ferric(Fe3+) by potassium ferricyanide measuring by spectrophotometer absorption 540nm
40
Caculation of Drabkin’s method
Hb-cc unknown(g/l)=(E unknown/ E standard)*HB-cc standard
41
Normal range of Hb concentration
Male:120-160 Female:140-180g/l
42
MCV mean corpuscular volume
=hematocrit/RBC count Normal range 82-92fl (f=10^-15)
43
MCH mean corpuscular hemoglobin
Hb cc/ RBC count Normal range: 28-36pg/RBC (p=10^-12)
44
MCHC mean corpuscular Hb cc
Hbcc/ hematocrit (gHb/RBC) Normal:310-360gHb/RBC

Physiology 1 Lab (5 decks)

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