Week 3 Flashcards
Fungal cell structure
Fungal cell nutrition
Heterotrophic: nutrients from dead organic matter or living organisms (exoenzymes digest outside to absorb nutrients)
Fungal morphology
Yeasts - oval/spherical
Moulds: filamentous/hyphal forms
Dimorphic: grow as yeast or mould depending on temp
Differentiation of fungi
Size + appearance
Colour
Surface elevations + depressions
Patterms
Examination of spores
Moulds - hyaline (colourless) or dematiaceous (pigmented), hyphal structure (spiral, racket shape)
Canine heartworm lifecycle and outcome
Dirofilarias immitis
Lifecycle: microfilariae in blood (L1) -> female mosquito (L1 -> L3, now infectious) -> mosquito injects L3 by biting -> L4 -> L5 -> adult in right ventricle and pulmonary vessels -> 6-7 month prepatent period
Following D. immitis:
Narrowing of pulmonary artery (medial layer enlarges -> hypertension)
Hydrothorax and hydroperitoneum
Haemoptysis - coughing up blood
Testing for parasites
Haemotology (increased eosinophil + basophil)
Thoracic radiographs (R heart enlargement)
ECG + echocardiogram
ELISA
Indirect fluorescent antibody (IFA)
Tracheal wash
Faecal egg count
Treatment of parasites
Adult worms - immiticide
Microfilariae - ivermectin (also used as a preventative)
Adulticides - dead worms induce inflammatory response -> pulmonary thrombolism (pulmonary artery clot)
Surgical removal - alligator forceps
GI parasite lifecycle
Adult worms lay eggs in intestines which are passed out in faeces
Eggs develop into larvae and are ingested by animal
Larvae migrate through intestine wall into bloodstream -> liver -> lungs
Migrate up trachea and swallowed (returning to intestine)
OR
migrate through large vessels and become lodged in small vessels -> vessel wall damage + blood clots
e.g. Strongylus Vulgaris (horses)
Heartworm in cats
Fewer worms in cats can cause significant disease
-> enlarged lung
Diagnosis difficult (usually amicrofilaraemic and low worm burden so ELISA test not sensitive enough)
Haematological abnormalities associated with parasitic disease
Anaemia - some parasites feed on blood
Eosinophilia
Thrombocytopenia - decreased platelets
Leukocytosis - increased WBCs
Hypoalbumenia - decreased serous albumin levels
Parasites that migrate through the lung
Dog heartworm - D.immitis
Dog lungworm - A. vasorum
Cat lungworm - A. abstrustus
Horse GI parasite - S. vulgaris
Cattle lungworm - D. viviparus
Sheep + goat lungworm - D. filaria
Routes of migratory parasites
Oral
Skin
Transplacental
Blood-borne
Through body cavities
Ingestion of intermediate hosts
Anthelmintics
Drugs used to kill parasites
Worming in horses
up to 6 months - every 2 months
6 months to 1 year - every 3 months
yearlings - every 4-6 months
Parasite direct and indirect life cycle
Direct - parasitic stages develop in/on host
Indirect - intermediate host required for parasite development