week 10 - metabolic disorders 2 Flashcards

1
Q

What is Glycogen Storage Disease (GSD)

A

Genetic condition that affects the enzymes involved in the storage or breakdown of glycogen.

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2
Q

Describe the structure and function of glycogen?

A

Branched polysaccharide of glucose.
Glycogen is a form of energy storage
Stored in liver and skeletal muscle

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3
Q

Give a brief overview of GSD type 1 aka Von Gierke’s disease

A

There are 2 subtypes:
GSD 1a is where there is an issue with glucose-6-phosphatase
GSD 1b is when there is an issue with glucose-6-phosphate translocase

Both the enzymes are needed for the release of glucose, so in GSD 1a and 1b there is a failure of glycogenolysis (breaking down glycogen to produce glucose)

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4
Q

Describe the genetics of GSD type 1

A

It is autosomal recessive - so parents can be carriers and not affected and give birth to an affected child

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5
Q

Describe how an affected baby in the womb may be healthy but when born starts to suffer from the disease

A

Because the baby in the womb has it’s mother’s glucose transferred to it via the placenta

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6
Q

What are the symptoms of GSD 1?

A

Primary symptoms:
Hypoglycaemia (low blood sugar)
Hepatomegaly (enlarged liver)
Increased infection (in 1b)

Secondary symptoms:
Increase in uric acid in the blood caused by low serum insulin
Increase in lactic acid in blood
Hepatic adenoma
IBS in 1b

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7
Q

Treatment for GSD type 1?

A

High glucose/high starch foods bc the body can’t produce glucose

Avoiding other sugars

Nocturnal gastric infusion

Filgrastim - treats low neutrophil count in GSD 1b by stimulating bone marrow

Gene therapy

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8
Q

What are the side effects of treatment for GSD 1?

A

Pancreatic hypertrophy - enlarged pancreas

Enlarged spleen, low platelet count, anaemia in 1b

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9
Q

What is the pathophysiology of GSD 1?
(what can GSD 1 lead to)

A

Hypoglycaemia
Lactic acidosis
Hyperuricemia
Hepatomegaly
Hepatic adenoma

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10
Q

How does hypoglycaemia (low blood sugar) occur in GSD 1?

A

Because glucose can’t be produced as there is failure in the enzymes that break down glycogen into glucose. So therefore there is low glucose sugar in the blood

The only way to increase blood glucose is via a high glucose diet.

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11
Q

How can lactic acidosis arise in GSD 1?

A

Lactic acidosis is an increase in lactic acid in the blood.

It occurs from impaired gluconeogenesis (new glucose being made)

It occurs when there is an accumulation of G6P which inhibits the conversion of lactate to pyruvate.

Causes discomfort in muscles

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12
Q

How does hyperuricemia occur in GSD 1?

A

This is where there is high levels of uric acid in the blood.
The high levels of uric acid are caused by the kidneys not being able to excrete enough uric acid.
This can lead to kidney stones, gout etc

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13
Q

How does hepatomegaly arise in GSD 1?

A

It is when you have an enlarged liver.
It is caused by an accumulation of glycogen in the liver as it isn’t being broken down into glucose.
Causes a protruding abdomen

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14
Q

How does hepatic adenoma arise in GSD 1?

A

It is a benign tumour of the liver, aetiology unknown.
Can progress to carcinoma.
You can reduce the size of the tumour by controlling your blood glucose levels.

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15
Q

Ho is GSD 1 diagnosed?

A

Genetic testing
Blood tests to check lactate, uric acid, glucose levels
Check neutrophil count as low neutrophil count only affects 1b
Biopsy of the liver

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16
Q

Give a brief overview of GSD type 2? aka Pompe disease

A

An autosomal recessive disease
It is caused by a deficiency in alpha-glucosidase (GAA)
It impairs glycogenolysis (breaking down glucose) because GAA usually degrades glycogen polymers to glucose in the lysosomes,
so if there is a deficiency in GAA glycogen cannot break down in to glucose
damages muscle cells

17
Q

What are the symptoms of GSD 2?

A

Infantile symptoms:
Muscle weakness
Respiratory deficiency
Hypertrophic cardiomyopathy
Swallowing difficulty
Hearing loss
Hypotonia (low levels of muscle tone)

Late onset symptoms:
Muscle weakness
Respiratory weakness
Balance issues
Scoliosis
Ptosis (drooping eyelids)

18
Q

Treatment for GSD 2?

A

Enzyme replacement therapy
High calorie diet
Speech therapy
Physiotherapy
Respiratory assistance

19
Q

What is the pathophysiology of GSD 2?

A

It can cause enlargement of the lysosomes and can cause them to rupture./
It disrupts the contractile units of the muscle leading to wasting

20
Q

How can you diagnose GSD 2?

A

Take a blood test to check GAA activity
Genetic testing for GAA mutations
Skin biopsy for GAA activity

21
Q

Give a brief summary of GSD type 4? aka Andersen disease

A

It is autosomal recessive
It is when there is a deficiency in the Glycogen branching enzyme (GBE)

GBE adds branching to growing glycogen polymers, in GSD 4, abnormal glycogen accumulates which damages liver and muscle cells

22
Q

What are the symptoms of GSD 4?

A

Progressive hepatic type:
Liver fibrosis
Cirrhosis
Hepatosplenomegaly
Reduced growth
Build up of fluid in abdominal cavity

Neuromuscular variants:
Muscle weakness
Muscle wasting
Severe cardiomyopathy
Heart enlargement due to weakness in heart muscle
Oedema

23
Q

How can you treat GSD 4?

A

Liver transplant
Manage Cirrhosis and heart dysfunction

24
Q

What is the pathophysiology of GSD 4?

A

It can cause an accumulation of structurally abnormal glycogen which interferes with liver function.
Leads to scar tissue

25
How can you diagnose GSD 4?
By carrying out a GBE activity assay Genetic testing for GBE mutations Liver function tests Abdominal ultrasound or CT scan
26
Give a brief summary of GSD 5 aka McArdle disease?
It is autosomal recessive Occurs when there is a mutation in the muscle isoform of glycogen phosphorylase (PYGM) The function of PYGM is to catalyse glycogenolysis (the breaking up of glycogen to form glucose) This causes muscle weakness and pain
27
What are the symptoms of GSD5?
Progressive hepatic type: Excessive intolerance Myalgia Fatigue Painful cramps Myoglobinuria (when there is myoglobin in the urine due to break down of skeletal muscle) Long term: Kidney failure Progressive weakness muscle loss
28
How can you treat GSD 5?
Exercise plan but avoid anaerobic exercise Consume glucose prior to exercise - this is so that there will then be glucose available in the blood bc with this disease glucose cannot be produced from glycogen Creatine supplements Maintain a balanced diet
29
What is the pathophysiology oof GSD 5?
Accumulation of intramuscular glycogen Lack of glucose for fuel in muscles Rhabdomyolysis - breakdown of skeletal muscle which releases myoglobin Kidney failure due to myoglobin accumulation in the kidneys tubules
30
How can one diagnose GSD 5?
Muscle biopsy to detect PYGM Genetic testing for PYGM mutations Forearm ischaemic exercise test - there is increased ammonia and reduced lactate in blood in patients with GSD 5 Check the resting creatine kinase level - is increased in patients with GSD 5
31
Give a brief summary of the mitochondria
It is an intracellular organelle which generates energy (ATP) for the cell Not found in red blood cells
32
What are 2 mitochondrial disease examples?
1. Leber Hereditary Optic Neuropathy 2. Mitochondrial Neurogastrointestinal Encephalopathy (MNGIE)