WEEK 1: Microscopy

Question 1

An instrument used to observe microorganism that cannot be seen by our naked eye.

Answer 1

Microscope

Question 2

A microscope that is based on the interaction of light and tissue components and can be used to reveal and study tissue features.

Answer 2

Light Microscopy

Question 3

In histology, this is made to make various tissue components conspicuous, but permit distinctions on the cell organelles.

Answer 3

Histological Stain

Question 4

Tissue components with positive charge stain more with basic dyes, known as _____; proteins with many ionized amino groups (negative charge), have the affinity for acidic dyes, know as ________

Answer 4

basophilic; acidophilic

Question 5

A microscope that allows light rays to pass directly through to the eye without being deflected by an intervening opaque plate in the condenser. A conventional type of instrument encountered by students.

Answer 5

Brightfield Microscope

Question 6

Parts of a Brightfield Microscope. Support the microscope; all parts are attached. Includes the arm and base.

Answer 6

Framework

Question 7

Parts of a Brightfield Microscope. The horizontal platform that supports the microscope slide.

Answer 7

Stage

Question 8

Parts of a Brightfield Microscope. Used for holding and moving the slide around on the stage.

Answer 8

Mechanical Stage - Clamping device

Question 9

Parts of a Brightfield Microscope. Adjust the position of the mechanical stage.

Answer 9

Mechanical stage control

Question 10

Parts of a Brightfield Microscope. Holds the specimen in place.

Answer 10

Stage clip

Question 11

Parts of a Brightfield Microscope. Allows passage of light from the light source.

Answer 11

Stage hole/ aperture

Question 12

Parts of a Brightfield Microscope. Illuminates the specimen.

Answer 12

Light source/ Illuminator

Question 13

Parts of a Brightfield Microscope. Adjust the intensity of light.

Answer 13

Voltage regulator

Question 14

Parts of a Brightfield Microscope. Adjustments are made on the left eye by turning the knurled ring on the left ocular until a sharp image is seen.

Answer 14

Diopter Adjustment Ring

Question 15

Parts of a Brightfield Microscope. consists of two or more internal lenses and usually has a magnification (10x).

Answer 15

Eyepiece/ Oculars

Question 16

Parts of a Brightfield Microscope. Attached to the revolving nosepiece. Has 4 objectives.

Answer 16

Objectives

Question 17

Objectives. Has 4x magnification

Answer 17

Scanner

Question 18

Objectives. Has 10x magnification

Answer 18

Low Power Objective (LPO)

Question 19

Objectives. Has 40x magnification.

Answer 19

High Power Objective (HPO)

Question 20

Objectives. Has 100x magnification.

Answer 20

Oil Immersion Objective (OIO)

Question 21

Formula for Total Magnification

Answer 21

Ocular lens x Objective Lens.

Question 22

Parts of a Brightfield Microscope. Collects and directs the light from the light source to the slide being studied.

Answer 22

Condenser

Question 23

Parts of a Brightfield Microscope. Found within the condenser that regulates the amount of light that reaches the slide.

Answer 23

Diaphragm/ Iris Diaphragm

Question 24

Parts of a Brightfield Microscope. Where the objectives are attached.

Answer 24

Revolving Nosepiece

Question 25

Parts of a Brightfield Microscope. Initially brings the specimen into focus.

Answer 25

Coarse Adjustment Knob

Question 26

Parts of a Brightfield Microscope. Sharpens the focus.

Answer 26

Fine Adjustment Knob

Question 27

Refers to objectives that can be changed with minimal or no refocusing. Allows objects to be visualized on a single focal plane

Answer 27

Parfocal