Wed skildum virus review

Question 1

trophism of EBV

Answer 1

lytic in epithelial cells of oropharynx

latent in b cells

Neumonic:
Epstein - epithelial
Barr - b cells

remember - EBV is mono/kissing disease

Question 2

EBV infection of the b cell makes copies of what - the virus or the provirus?

Answer 2

the provirus. this is what it means to be latent

Question 3

what causes the agglutination in a mono spot test

Answer 3

heterophile antibodies made by b cells. heterophile just means these antibodies respond to a lot of things.

Question 4

3 EBV oncogenesis genes that we need to know

Answer 4

EBNA3C - (epstein barr nuclear antigen 3C) - activates cyclin D1 complex, leads to hyperphosphorylation of retinoblastoma protein, activates E2F, leads to progression of cell cycle.

LMP-2 - mimics the active B cell receptor, which promotes MAPK and fos/jun gene activation

LMP-1 - functions as constitutively active CD40 on B cells. binds to CD40L on the t-cells which causes proliferation of the b-cells by activating transcription of NF-kappaB related genes

Question 5

Appearance of what in a blood smear is diagnostic for EBV

Answer 5

atypical lymphocytes called Downey cells

Question 6

X linked lymphoproliferative disorder

Answer 6

"fulminant" infection mono
rare mutation that makes it hard to fight EBV
~3 years old
1-2 months survival
x linked

mutation that results in non-functional SAP protein (SAP normally binds phosphorylated tyrosines on SLAM (CD150) on t cells which allows IL-4 production in t cells, which allows the B cell to class switch

*impaired activated induced cell death, you get too many b cells of the same type, which are not very useful. Immune response has no breaks