We CAN unfold Proteins if they Were Folded Flashcards

1
Q

Proteins can be unfolded

A

Denatured

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2
Q

3 Characteristics of unfolding proteins

A

1: Lost most levels of structure
2: Adopts random coil conformation
3: Maintains the primary amino acid sequence

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3
Q

Denaturing agents

A

Interferes with the FORCES✊🏻 that stabilize protein folding

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4
Q

Denaturing Agents

A
Heat (irreversible) 
Agitation
pH
B-mercaptoethanol
Detergents
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5
Q

Target

A
H-bonding, hydrophobic interac. 
H-bonding, hydrophobic interac. 
Salt bridge 
Disulfide bond 
Hydrophobic interac.
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