Denaturation is Reversible Flashcards
1
Q
An enzyme that cleaves Dna
A
Renaturation of Ribonuclease A
2
Q
1.) Treatment with Urea and B-Mercaptoethanol can give a ________, denatured protein.
A
Completely unfolded
3
Q
B-Mercaptoethanol
A
Reduce disulfide bond
4
Q
Urea
A
- Breaks H-bonds
- Hydrophobic interac.
5
Q
2.) He removed the ______________ and exposed the ______________ to air.
A
Denaturants
protein
6
Q
3.) The protein _____________ into its original _______________ and activity was restored.
A
Folded back
3-D shape
7
Q
*The unfolded polypeptide ____________ by itself in the test tube. *
A
Refolded
8
Q
Conclusion: How a protein folds is encoded ____________.
A
Depending on the primary sequence.
It dictates 2 and 3 structure.
1——> 2 ———>3
