Wbc Flashcards
Arneth count/index
It’s based on the principle that more the lobe more mature the neutrophils
Divided into 5 stages
1-5% single lobed
2-30%2 lobe
3-45% 3lobe
4-18% 4 lobe
5-2% 5 lobe
What do right shift in arneth count indicate
Stages 4 &5 anemia
Left shift in arneth count?
Stage 1&2 infection
Schilling index
Categorised into 4 groups based on the polymorphous neutrophils
Dimensions of Barr body
Drumstick shaped 0.7-1.2micron
Total time of normal granulopoises
10 days
In which condition time for wbc maturation is decreased
At the time of infection/inflammation
Reduces the post mitosis phase
Describe the life cycle of granulocyte
Myeloblast-promyelocyte-myelocyte-metamyelocyte-band form-mature wbc
RBC:wbc
600:1
In bone marrow ratio of myeloid:erythroid
3:1
What are the steps of phagocytosis
1.margination
2.diapedesis
3.chemotaxis
4.opsonisation
5.engulfing and degranulation
6.bacterial killing
Examples of chemotaxins
1.some bacterial toxins
2.degenerate product of inflammed tissues
3.component of complement system
4.product of lipoxigenase path way
5.cytokines IL-8 &platelet activation factor
6.polypeptide from lymphocytes,mast cells
Examples of opsonents
IgG opsonin
Opsonin fragment of complement protein
What is respiratory burst
Cause high o2 intake and high increase in metabolism
which are the three oxidative bacterial killing enzymes
NADPH oxidase
Super oxide dismutase
Myeloperoxidase
Oxygen independent anti microbial system
1.acidification of phagosome
2.lysozyme
3.lacto ferrin /inhibit bacterial growth
4.defensive.microbicidal peptide
5.bacterial permeability increases
In the step of marginasation
Factors present in the
A.neutrophil
B.cell membrane
A:integrin
B:selectin &Icam-1
What are the changes occuring to the neutrophil during chemotaxis
Changes its shape and become amoeboid
What is opsonisation
Process by which bacteria is made tasty is known as
