WBC

Question 1

SI unit for adult WBC

Answer 1

4.5 to 11 x10^9/L

Question 2

Conventional unit for newborn WBC count

Answer 2

13,500 - 38,000 per mm^3 or uL

Question 3

Most reliable criteria to determine WBC maturity

Answer 3

Nuclear chromatin pattern

Question 4

The presence of Type 3 myeloblast is commonly associated with:

Answer 4

Acute myeloid leukemia

Question 5

Cite the general characteristics as granulocytes mature: (5)

Answer 5

Chromatin condenses, nucleoli disappear, abundant basophilic cyto (nonspecific granule) becomes scanty with specific granules, nuclei indents & segment, cell size decreases

Question 6

The earliest recognizable WBC granulocytic precursor

Answer 6

Myeloblast

Question 7

This type of granulocytic precursor has azurophiic granules with more than 20. Purple cytoplasm and a dark chromatin

Answer 7

Type 3

Question 8

Type 1 myeloblast are characterized by

Answer 8

Little cytoplasm (slightly basophilic), 2-4 nucleoli, no visible granule, fine nuclear chromatin

Question 9

The presence of ____ in promyelocytes indicates normality of the cell

Answer 9

Hof

Question 10

This precursor is larger than its previous precursor.

Answer 10

Promyelocyte

Question 11

Absence of hof may indicate what disease

Answer 11

Acute promyelocytic leukemia

Question 12

How many nucleoli may be seen in promyelocytes. Do they obscure the nucleus?

Answer 12

1-3

They may obscure

Question 13

This precursor stage is where secondary specific granules are produced.

Answer 13

Myelocytic

Question 14

What type of myelocyte looks similar to promyelocytes. How to distinguish it?

Answer 14

Early myelocyte.

It has patches of grainy pale pink cytoplasm - dawn of neutrophilia (golgi body)

Question 15

Which type of myelocyte is smaller than promyelocyte? What are its other characteristic

Answer 15

Late myelocyte. More heterochromatin, nucleoli hard to visualize

Question 16

Last stage capable of mitosis

Answer 16

3rd- myelocyte

Question 17

This precursor must only be found in the BM among healthy individuals

Answer 17

Metamyelocyte

Question 18

Synthesis of gelatinase granules are in this precursor stage

Answer 18

Metamyelocyte

Tertiary granule

Question 19

Youngest precursor to normally appear in the PBS

Answer 19

Band cell/ stab/ staff

Question 20

Secretory vesicles/ granules are formed in this stage

Answer 20

Band cell

Question 21

How to differentiate Metamyelocyte from band cell?

Answer 21

Metamyelocyte - <1/2 nuclear indentation - shallow, peanut or kidney bean shape
Band celll - > 1/2 indent; sausage shape

Question 22

What precursor is often mistaken as a band cell?

Answer 22

Metamyelocyte

Question 23

Agranulocytosis has a neutrophil count of _____ commonly associated by: _____ & _____

Answer 23

(<0.5 x 10^9/L)

Amidopyrine and cephalosporin

Question 24

Which disease/infection produces the highest EOSINOPHIL count

Answer 24

Trichinosis

Question 25

Mod to severe eosinophilia is commonly associated with

Answer 25

Helminthic infection (nematode, cestode, trematode)

Question 26

Most predominant type of lymphocyte

Answer 26

Small (7-10 um) - mostly nucleus, scanty cytoplasm

Question 27

Final maturation stage of B lymphocyte

Answer 27

Plasma cell

Question 28

Nuclear & cytoplasmic characteristic of plasma cells

Answer 28

Nuclear - oval, eccentric, tortoise shell, cartwheel, clock face
Cyto - dark or sea blue, cornflower color, with round discrete russel body containing Ig’s

Question 29

Commonly associated disease of plasma cells

Answer 29

Multiple myeloma (plasma cell myeloma) - Bence Jones protein

Question 30

Most abundant cell in the body

Answer 30

Macrophage

Question 31

Functions (4) of macrophages

Answer 31

Phagocytose, nitric oxide (cytotoxic), IL-1 (stimulate T cell), transcobalamin 2 (primary transport vit b12)