Virus Quantitation - Lec. 5

Question 1

Which of the following is not a physical assay for quantification of viruses ?

A. Electron Microscopy
B. ELISA
C. Pock assay
D. qPCR

Answer 1

C. Pock assay

Question 2

A PFU/ml of 300 means _______

A. 300 viruses are present in 1ml of sample to cause one plaque in monolayer cell culture
B. One virus is present per ml of sample to form 300 plaques in monolayer cell culture
C. The minimum # of viruses present per ml of the sample to from 300 plaques in monolayer cell culture

Answer 2

C. The minimum # of viruses present per ml of the sample to from 300 plaques in monolayer cell culture