Virology Flashcards
Soon after interaction with the host cell vision is disrupted infetivity is lost
Eclipse period
Virus replication cultured only on
Monkey kidney cell
Duck egg cell
Rhinovirus host receptor
ICAM 1
HIV host receptor
CD4 +T cells
Host receptor
Rabies virus
ACH receptors
Host receptor
HSV 1
Fibroblast growth factor receptor
Appropriate pH and ionic concentration require counter ion
Magnesium ion
Refers to the entry of viral particle into the cytoplasm of the host cell
Penetration
Penetration carried out through
Endocytosis ( receptor mediated) Direct penetration ( viropexis) Cell fusion ( giant cells )
Refers to the physical separation of the viral capsid from the viral genome
Uncoating
Steps in viral infection
Attachment and absorption Penetration Uncoating Early viral mRNA synthesis Early viral protein synthesis
Infectious nucleic acid
If - stranded virus
Non infectious nucleic acid
Can bypass the host range specificity provided by the protein host cell receptor interaction
Eg poliovirus
1st step in gene expression
mRNA synthesis
DNA viruses except ——- replicate in the nucleus , use host cell DNA dependent RNA polymerase synthesize mRNA
Pox virus
DNA virus are double stranded
Both + and - strands used as templates
Doesn’t have access on the RNA polymerase on host cell nucleus
Pox virus
Pox virus should have RNA polymerase
Synthesize mRNA
Pox virus replication
Cytoplasm
RNA viruses are single stranded
Can be positive or negative
Used as template can synthesize RNA
Positive polarity RNA viruses
Picornaviruses
Togaviruses
Flaviviruses
Negative polarity
Synthesize own mRNA
Orthomyxovirus
Paramyxovirus
Fill virus
Bunyavarius
Negative polarity must be converted to positive strand through
RNA -RNA dependent mRNA -> transcription
2 processes of release
Rupture or lysis of cell membrane
Budding through the outer membrane