virology Flashcards
1
Q
frdefine the term phylogenetics
A
routine identification of an outbreak source
2
Q
what is chain termination sequencing also known as?
A
sanger sequencing
3
Q
what is used in sanger sequencing?
A
different length DNA fragments generated by dye-labeled dideoxynucleotides
4
Q
pyro sequencing or ‘sequencing by synthesis’ is also know as what
A
2nd generation sequencing
5
Q
what does Roche 454 detect?
A
pytophosphate release as nucleotides incorporated into new DNA strands-FLASH OF LIGHT
6
Q
H+ (proton) release is detected by what type of pyrosequencing? (use of pH)
A
Ion torrent
7
Q
illumina/solexa detect what unique signal?
A
fluorescent
8
Q
A
