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Microbiology (PRCI) > Virology > Flashcards

Virology Flashcards

1
Q

Lipid rich; host cell dervied

A

Envelope

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2
Q

Envelope

Ether stable:
Ether labile:

A

: Naked virus
: Enveloped virus

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3
Q

Compkete viral particle

A

Virion

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4
Q

Replication site
DNA:
RNA:

A

Nucleus
Cytoplasm

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5
Q

Delay in processing

Store at:
>4days delay maintain at:

A

4C
-70C

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6
Q

Transport temperature for viral culture

A

Refrigerator

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7
Q

Keeps tissue moist

A

Viral transport media
Saline
Trypticase soy broth

Ex.
- Stuart’s
2% fetal calf serum with Eagle’s minimum essential medium

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8
Q

Specimens that require VTM

A

Swabs, respiratory specimens, tissue specimens

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9
Q

When to collect viral specimens?

A

3-4days after onset of signs and symptoms

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10
Q

Isolation of virus
Nasopharyngeal swabs:
Nasal swab:

A

Respiratoty syncytial virus, influenza,
Parainfluenza
Rhinovrus

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11
Q

Anticoagulant for nucleic acid testing of blood specimens

A

EDTA

SPS/Heparin - culture

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12
Q

Cytopathic effect of rabies

A

Negri bodies

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13
Q

CPE of CMV

A

Owl’s Eye nuclear inclusions

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14
Q

CPE of adenovirus

A

Grapelike clusters, smudge cells

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15
Q

CPE of Vaccinia and variola

A

Guarnieri body

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16
Q

Koilocytes; weinkled prune nucleaus in Paps smear

A

HPV

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17
Q

Flower nuclei in T cells

A

HTLV-1

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18
Q

CPE of Measels

A

Warthin Finkeldey cells

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19
Q

Cowdry Type B cells

A

Enterobirus

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20
Q

Councilmann bodies

A

Yellow fever

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21
Q

3M’s is the CPE of

A

HSV

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22
Q

Lipschultz body, patterson bodies

A

Molluscum contagiosum

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23
Q

Decoy cells

A

BK virus

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24
Q

Bizarre astrocytes; oligodendrocyte nuclei enlarged

A

JC virus

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25
Q

Viral antigen method for HSV

A

Enzyme linked virus inducible system (ELVIS)

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26
Q

Gold standard for viral diagnosis

A

Cell culture

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27
Q

Cell culture
DNA virus grow better in:
RNA virus grow better in:

A

MRC5
Rhesus monkey kidney (RMK)

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28
Q

Viral diagnosis incubation temperature

A

35-37C (mostly); 33C (respiratory viruses)

For 1-4 weeks; usually 2 weeks

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29
Q

Fast growers (1-3 days):
Moderate growers (2-10 days):
Slow growers (7-14 days):

A

HSV
Influenza, entero irus, adenovirus, mumps, measles, PIV, RSV
VZV, CMV

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30
Q

Dewdrop on rose petal lesion

A

Chickenpox - HHV3 / Varicella Zoster Virus

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31
Q

Atypical cells: Downey cells (ballerina skirt appearance)

A

Epstein barr virus

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32
Q

Orbiting satellite

A

Adenovirus

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33
Q

Water warts / Cup-shaped crater

A

Molluscum contagiosum

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34
Q

Diagnosis of monkeypox

A

RT-PCR

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35
Q

Serotypes of genital warts

A

6, 11

Cervical cancer: 16, 18

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36
Q

Progressive multifocal leukoencephalopathy

A

JC virus

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37
Q

Smallest DNA virus

A

Parvoviridae

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38
Q

5th disease / erythema infectiosum

A

Parvovirus B19

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39
Q

Slapped cheek appearance, lacy pattern erythema, gloves and socks syndrome

A

Parvovirus B19

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40
Q

Hemorrhagic cystitis

A

BK virus

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41
Q

Largest RNA virus

A

Paramyxoviridae

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42
Q

Smallest RNA virus

A

Enteroviridae (Picornaviridae)

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43
Q

Overall largest virus

A

Poxiviridae

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44
Q

Segmented viruses

A

Retroviridae
Arenaviridae
Bunyaviridae
Orthomyxoviridae
Reoviridae

45
Q

Arthripod borne viruses (ARBOVIRUSES)

A

Bunyaviridae
Flaviviridae
Togaviridae
Reoviridae

46
Q

Picornaviridae

Acid labile:
Acid stable:

A

Rhinovirus
Enterovirus

47
Q

Targets anterior horn of spinal cord

A

Poliovirus

48
Q

Hand foot and mouth disease

A

Coxsackie A

49
Q

Devil’s grip or Pelurodynia

A

Coxsackie B

50
Q

Summer diarrhea

A

Parechovirus of Picornaviridae

51
Q

LJungan virus; zoonotic

A

Parechovirus B

52
Q

Polio Vaccines

Salk ______ and Sabin _______

A

Salk: dead, venous
Sabin: live, oral

53
Q

Most common cause of viral heart disease

A

Coxsackie B

54
Q

Common agent of aseptic meningitis

A

Enterovirus

55
Q

Echovirus name

A

Enteric cyopathic orphan virus

56
Q

Sandy and granular

A

Arenaviridae

57
Q

Hemorrhagic fever assoc w Arenaviridae

A

Lassa Fever (BSL4)

58
Q

Post-mortem diagnosis

A

Arenaviridae

59
Q

Lymphocytic choriomeningitis

A

Arenaviridae

60
Q

Lymphocytic choriomeningitis

A

Arenaviridae

61
Q

Epidemic gastroenteritis on adults (cruisehips)

A

Norovirus / Norwalk Virus

NORO Aunor
SAPOnINfants

62
Q

Winter vomiting disease

A

Norovirus /Norwalk

63
Q

Seen in electron microscope for Calciviridae

A

Star if David

64
Q

Novel Coronaviridae

A

SARS-COV
MERS-COV
SARS-COV2

65
Q

Petal shaped / club shaped spikes

A

Coronaviridae

66
Q

Receptor for coronaviridae

67
Q

Covid-19 testing
RT-PCR target:
Ag testing target:

A

ORFLAB, n, E
Mucleocapsid protein Ag

68
Q

Thread- like; filamentous virus

A

Filoviridae

69
Q

Most deadly Ebola virus

A

Ebola Zaire

70
Q

Non pathogenic Ebola virus

A

Ebola Reston

71
Q

Shepered’s crook seen in

A

Filoviridae

72
Q

Biosafety level for Ebola

73
Q

Croup

A

Parainfluenza virus

74
Q

Hallmark feature of Measles - Rubeola

A

Koplik spots (grains of salt on a red background)

75
Q

Severe measles associated with

A

Vit A deficiency

76
Q

Stensen’s duct spxn for

77
Q

Cytopathic effect if measles

A

Giant cells

78
Q

Common cause or viral pneumonia among children 5yrs and below

A

Respiratory syncytial virus

79
Q

Reye’s syndrome

A

Influenza B

80
Q

Defective interfering particles in orthomyzoviridae

A

Von Magnus particle

81
Q

Bullet shaped RNA virus

A

Rhabdoviridaw

82
Q

Causes rabies virus

A

Rhabdoviridae

83
Q

Viral Stomatitis Virus belongs to family

A

Rhabdoviridae

cattle

84
Q

Confirmation of rabies virus infection

A

Biopsy, post mortem brain exam

85
Q

Virus associated with
Renal involvement:
Pulmonary involvement:

A

Hantavirus
Sin Nombre

86
Q

Exposure to deer mice droppings

A

Hanta virus

87
Q

Black vomit

A

Yellow fever - Flaviviridae

88
Q

Most rapid spreading mosquito borne disease

A

Denge Fever

89
Q

Breakbone fever

90
Q

Same with dengue but MOT is sexual contact

91
Q

Flaviviridae infection associated with Guillian barre syndrome

92
Q

Surrogate marker of HCV

93
Q

Most important cause of infantile diarrhea (double capsid)

94
Q

“To be contorted”

A

Chikungunya

same vector w dengue

95
Q

Forscheimer / Forchheimer spots (rose colored spots on palate)

A

German Measles / Rubella / 3-day. Easles

96
Q

Blueberry muffin

A

Rubella / 3-day measles / German measles

97
Q

AIDS related virus

A

HIV-1 / HTLVIII

98
Q

Seen in hairy cell leukemia

99
Q

Prevalnt lentiviridae

100
Q

Most common subtype of HIV-1

101
Q

Target cell of HIV1

A

T-helper cells, monocytes, macrophages, dndritic cells

102
Q

CD4 count of AIDS Px

A

<200cells/uL

CD4:CD8 = 1:2 or 0.5:1

103
Q

CPE on HTLV1 PBS

A

Flower cells

104
Q

CPE on HTLV1 PBS

A

Flower cells

105
Q

HIV
Receptor:
Co-receptors:

A

CD4
CXCR4 ND CCR5

106
Q

US CDC Guidelines
Screen w/
If (+) =
If indeterminate =

A

4th generation test (Detection of Ag and Ab)
5th generation test
NAAT

107
Q

Western blot positive:

A

2 out of 3 bands (p24, gp41, gp120/160)

108
Q

Proteins of prions

Microbiology (PRCI) (5 decks)

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