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Microbiology (PRCI) > Mycology > Flashcards

Mycology Flashcards

1
Q

Group of yeast that produce pseudohypha

A

Candida (except C. globrata)

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2
Q

Mold or Yeast:
Alcohol like odor

A

Yeast

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3
Q

Dimorphic fungi forms:
- body temp:
- room temp:

A

Yeast
Mold

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4
Q

Fungi that usually cause systemic mycoses

A

Dimorphic fungi

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5
Q

Unique at yeast form; not true thermally dimorphic fungi; hallmark of infection

A

Spherule

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6
Q

Group of fungi that has no sexual mode of reproduction

A

Fungi imperfecti

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7
Q

Spindle shaped conidia

A

Macroconidia

large, multicellular

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8
Q

Conidia that is formed during unfavorable conditions/ environment

A

Chlamydoconidia

(Terminal, intercalary, sessile)

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9
Q

Barrel shaped spores (rectangular)

A

Arthroconidia

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10
Q

Spores that possesses dysjunctor cells - gives checkered appearance

A

Arthroconidia

(with empty spaces)

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11
Q

Geotrichum

A

Hockey-stick appearance

(no empty spaces)

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12
Q

Fruiting bodies composed of asci

A

Ascocarps

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13
Q

Homothallic spores:
Heterothallic spores:

A

Zygospores
Oospores

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14
Q

Delays (storage)
Sterile specimen:
Non sterile specimen:

A

37C
4C

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15
Q

Antibiotic that can inhibit yeast phase of a dimorphic fungi

A

Chloramphenicol

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16
Q

Dermatophytes

Wood’s lamp:
POSITIVE -
NEGATIVE -

A

Microsporum (Ectothrix)
Trichophyton (Endothrix)

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17
Q

Agent added for too viscous respiratory specimens

A

NALC (N-acetyl-cysteine)

liquifying agent, digestant

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18
Q

Most common cause of fungemia (yeast, mold)

A

C. albicans
Fusarium (rare)

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19
Q

Anti-foaming agent for blood specimens using isolator tube

A

PEG (Polyethylene glycol)

Lysing agent - Saponin

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20
Q

Alternative for India Ink

A

Nigrosin

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21
Q

Mycotic infection features:
- spaghetti and meatballs
- bowling pin or medicine bottle

A

Tinea versicolor / Pityriasis Versicolor by Malassezia furfur

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22
Q

Scutula found in

A

T. favosa / Favus

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23
Q

Jock itch

A

T. cruris

Assoc w/ Epidermophyton floccosum

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24
Q

Athlete’s foot

A

T. pedis

Assoc with Epidermophyton floccosum

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25
Q

Microsporum ectothrix infection pattern

A

Mosaic pattern

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26
Q

Beaked apex

A

M. canis

curved terminal ends

M. gypseum / Nannizza gypsea - rounded terminal ends

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27
Q

Lemon yellow / Bright yellow (reverse) colonies

A

M. canis

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28
Q

Tan/buff; cinnamon colonies

A

Nannizza gypsea (M. gypseum)

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29
Q

Cottony white; salmon colonies

A

M. audouinii

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30
Q

Microsporum Rice agar NEGATIVE

A

M. audouinii

31
Q

Wood’s lamp negative microsporum

A

M. gypseum (Nannizzi gypsea)

32
Q

Snow paddle macrosporida

A

E. floccosum

33
Q

Khaki green; velvety colonies

A

E. floccosum

34
Q

Cutaneous mycoses with no microconidia

A

Epidermophyton

35
Q

Splendor hoeppli phenomenon seen in

A

Rose Gardener’s disease / Sporothricosis / Rose Handler’s Disease

36
Q

Rose Gardener’s dusease:
Mold form -
Yeast form-

A

Mold form - Daisy-head / Flowerette conidia
Yeast form - cigar-shaped

37
Q

Biopsy findings in Rose Gardener’s dse

A

Asteroid bodies

38
Q

Verrucoid nodunal Lesion

A

Lobomycosis / Keloidal blastomycosis

39
Q

Staining technique for lobomycosis

A

GMS (Grocott Methenamine Silver)

40
Q

Diagnostic fearure of chrommycosis

A

Sclerotic / Medlar bodies

copper penny

41
Q

Cauliflower-like lesions

A

Chromomycosis

42
Q

Diagnostic feature of mycetoma or madura’s foot

A

Grains or granules

43
Q

Mode of transmission of systemic mycoses

A

Inhalation of spores

Specimens: Respiratory; biopsy

44
Q

Exoantigen testing

A

For confirmatory of systemic mycoses

(+) = Specific A Band

45
Q

Mold form of Gilchrist’s dse

A

Lollipop conidia

46
Q

Mariner’s wheel / Mickey mouse cap

A

Paracoccidiodomycosis

47
Q

Exoantigen test result of Paracoccidiodomycosis

A

Band 1 , 2 ,3

48
Q

Most virulent gent of mycotic infection

A

Coccidioides immitis

49
Q

Exoantigens of San Joaquin valley fever / coccidioidmycosis

A

IDCF, HL, TP

50
Q

Exoantigen of histoplasmosis / Darling’s disease

51
Q

MOT of histoplasmosis

A

Inhalation of bat, bird guanos, chicken, starling droppings

52
Q

Etiologic agents of opportunistic mycoses

A

Saphrophytic fungi

53
Q

Used to differentiatw C. albicans to C. dublinensis

A

Incubation at 42C

C. albicans = (+)
C. dublinensis = (-)

54
Q

Animal reservoir of C. neoformans

55
Q

CALAS detects:

A

GXM Ag-glucuronoxylomannan

56
Q

Aflatoxin B poisoning seen in

A

Aspergillosis; Fungus ball

A. Flavus

57
Q

Animal reservoir of talaromycosis

A

Bamboo rat

58
Q

Honeycomb exudates

A

Pneumocystis pneumonia

59
Q

Punched pingpong ball

A

Pneumocystis pneumonia

60
Q

Lid lifters

A

Zygomycosis

61
Q

Clearing time of 10% KOH

A

10mins

Heat INCREASES rate of clearing

62
Q

If spxn is highly keratinized, what is the concentration of KOH

63
Q

Excellent mounting medium for most fungi; most widely used stain

A

Lactophenol cotton blue (clearing agent, fungicide, stain)

64
Q

Demonstrate encapsulated yeast

65
Q

Hucker’s modification of Gram stain

A

Crystal violet and ammonium oxalate

66
Q

Demonstrate melanin pigment

A

Masson Fontana

67
Q

General purpose culture medium

A

Sabouraud Dextrose Agar

68
Q

Dermatophyte test medium

pH indicator:
Dermatophytes:

A

Phenol red
Red

69
Q

Czapeck’s Agar

A

Aspergillus

70
Q

Birdseed agar

A

Cryptococcus

71
Q

Incubation temp for fungal diseases

A

Room temperature

72
Q

Culture # of days before reporting as NEGATIVE

A

30days (4 weeks/28days)

73
Q

Test to presumptively ID of C. albicans

A

Germ tube test

74
Q

Rapid ID of C. Neoformans

A

L-DOPA Ferric citrate test

Microbiology (PRCI) (5 decks)

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