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Microbiology (PRCI) > General Characteristics & Others > Flashcards

General Characteristics & Others Flashcards

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Biology 101 flashcards
1
Q

: Filaments of fungi

A

Hyphae

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2
Q

: Mats of hyphae

A

Mycelia

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3
Q

: viruses w bacterial host

A

Bacteriophage

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4
Q

: causes transmissible spongiform encephalopathy

A

Prions

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5
Q

Spirilla is motile by:
Spirochete is motile by:

A

Flagella

Axial Filaments

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6
Q

Cellular arrangement forming “Picket Fence” / “Chinese Character” appearance

A

Palisades

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7
Q

Test to identify encapsulated organisms

A

Neufeld Quellung Test

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8
Q

Teichoic acid in cell wall is a characteristic of gram ____

A

Positive

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9
Q

Beta-lactam antibiotics resistance:
G (+):
G(-):

A

Sensitive
Resistant

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10
Q

End product of Fluorochrome stains:
Acid-fast:
Non-acid fast:

A

Yellow fluorescence
Red fluorescence

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11
Q

Method which uses 0.5-1% sulfuric acid decolorizer

A

Modified Kinyoun Method

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12
Q

Organisms considered as mollicutes

A

Mycoplasma and ureaplasma

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13
Q

: temporary loss of cell wall

A

L-form

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14
Q

: site of energy metabolism

A

Plasma membrane

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15
Q

Point of attachment of chromosome

A

Mesosome

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16
Q

Metachromatic granules
- Babes Ernst Granules:
- Much granules:
- Bipolar bodies (Safety pin appearance):

A

: Corynebacterium diptheria
: Mycobacterium tuberculosis
: Yersinia pestis, Pasteurella

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17
Q

: flagella-like organelles in periplasmic space (not true flagella)

A

Endoflagella

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18
Q

: allows bacteria to resist heat and chemicals; found in the endospore

A

Calcium dipicolinate

19
Q

: temperature best for locomotion

A

25deg C (Mr)

20
Q

CHARACTERISTIC MOTILITY
: Listeria
: Campylobacter
: Capnocytophaga
: Proteus, C. Tetani, C. Septicum

A

: Tumbling
: darting
: gliding
: swarming

21
Q

Biosafety cabinet used for handling radioisotype carcinogens

A

Biosafety cabinet level II, B1 / B2

22
Q

HEPA means

A

High efficiency particulate air

23
Q

Minimum volume of specimen for body fluids sample

A

1mL

10-15mL for Fungal/Mycobacteria samples

24
Q

CSF sample order of collection:

A
  1. Chemistry
  2. Microbiology
  3. Hematology

CoMe Here

25
Q

1 bottle CSF specimen, order of sections for testing:

A

Microbio——Hema——Chem

26
Q

Preferred urine sample for anaerobic culture:

A

Suprapubic aspiration

27
Q

Stool to preservative ratio

A

1:3

28
Q

: method used for examination of motility

A

Hanging drop method

True Motility - characteristic motility
Brownian Motility - random movement

29
Q

Liquid media (+) growth result/reaction

A

Increase in turbidity

30
Q

: used to get rid of bubbles in culture media preparation

A

Pass the flame of a bunsen burner quickly over the surface of the agar

31
Q

: to prevent formation of water droplets

A

Store upside down

32
Q

: technique used for determining hemolysis pattern

A

Transillumination technique

light source from bottom of plate

33
Q

: alpha hemolysis surrounded by beta hemolysis

A

Alpha-prime / wide zone

Viridans group of Streptococcus

34
Q

: Double zone of hemolysis

A

Target hemolysis

Bull’s-eye
C.perfringens

35
Q

: lowest amount of antibiotic that prevents visible growth

A

Minimal Inhibitory Concentration (MIC)

36
Q

: concentration of antibiotic resulting in 99.9% reduction of CFU/mL

A

Minimal Bactericidal Concentration

37
Q

Standard-sized inoculum- compare broth to __________

A

0.5 McFarland Standard

bacterial suspension of 1.5x10^8 CFU/mL

38
Q

Zone of inhibition and MIC relationship

A

Inversely proportional

INC zone of inhibition = DEC MIC

39
Q

: used to induce resistance to clindamycin in D-test

A

Erythromycin

40
Q

: uses a plastic strip w/ antibiotic concentration gradient

A

E-test / Epsilometer test

41
Q

: supplements added to MHA in Oxacillin screen plate

A

4% NaCl and 6ug/mL Oxacillin

42
Q

Test to detect Methicillin-resistant S.aureus

A

Oxacillin screen plate

43
Q

Aerobic g(+) bacilli

A

STARiNG (branching)
Streptomyces
Tsukamurella
Actinomadura
Rhodococcus
Nocardia
Gordonia

LAGE BC (non-branching)
Listeria
Arcanobacterium
Gardnerella vaginallis
Erysipelothrix
Bacillus (spore former)

Microbiology (PRCI) (5 decks)

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