Viro7

Question 1

A cell in which a virus is able to replicate, i.e. the cell machinery supports replication of the virus is called:

Answer 1

A Permissive Cell

Question 2

Cells in which a factor or factors necessary to viral reproduction is not present or one detrimental to viral reproduction is present are called

Answer 2

Non-Permissive Cells

Question 3

Refers to the number of virions that are added per cell during infection

Answer 3

MOI (Multiplicity of Infection)

Question 4

In the One-Step Virus Growth Curve, the number of infectious virions released per average cell is called:

Answer 4

Burst size

Question 5

During this phase no extracellular virions are detected

Answer 5

Latent period

Question 6

Referring to a phase: The time from uncoating to just prior to the release of the first extracellular virions

Answer 6

Latent period

Question 7

A culture is inoculated with virions, but no infectious virus can be detected during this phase___________.

Answer 7

Eclipse period

Question 8

Time interval between uncoating [“disappearance” of viruses] and appearance, intracellularly, of first infectious progeny virions

Answer 8

Eclipse period

Question 9

During this phase, the titer of free virus in the medium declines.

Answer 9

Adsorption

Question 10

During this period, virus attaches to and enters cells

Answer 10

Adsorption

Question 11

A.

B.

C.

Answer 11

A. Adsorption

B. Eclipse

C. Burst

Question 12

The 6 steps in Virus Replication

Answer 12

1. Attachment
2. Penetration
3. Uncoating
4. Synthesis
5. Assembly and Maturation
6. Release

Question 13

What are the 4 methods of viral penetration?

Answer 13

1. Endocytosis
2. Surface Fusion
3. Pore-Mediated
4. Antibody-Mediated

Question 14

A process in which a substance gains entry into a cell without passing through the cell membrane

Answer 14

Endocytosis

Question 15

What are the 3 kinds of endocytosis

Answer 15

1. Phagocytosis
2. Pinocytosis
3. Receptor-mediated endocytosis

Question 16

A process by which cells absorb metabolites, hormones, other proteins that can be hijacked by a virus

Answer 16

Clathrin-mediated endocytosis

Question 17

The increase in local concentration of adaptor proteins on the inside face of the cell membrane allows clathrin to multimerize to form:

Answer 17

Clathrin-Coated Pits (CCP)

Question 18

Membrane scission proteins which pinch off the Clathrin-Coated Pits are called

Answer 18

Dynamin

Question 19

what is an endosomal signal which causes the release of enveloped viruses?

Answer 19

Low pH

Question 20

Some non-enveloped viruses induce __________of host endosomal membrane to allow virus capsid penetration into the cytoplasm

Answer 20

local permeabilization

Question 21

What are other types of Receptor-Mediated Endocytosis for which we don’t know the mechanism? “Jus remember the names”

Answer 21

1. Caveolin-mediated endocytosis of virus by host. (specialized lipid rafts)
2. Clathrin- and caveolin-independent endocytosis of virus by host

Question 22

Some non-enveloped viruses inject their genome into the host cytoplasm. What structure is used?

Answer 22

Pore