Viro7 Flashcards
A cell in which a virus is able to replicate, i.e. the cell machinery supports replication of the virus is called:
A Permissive Cell
Cells in which a factor or factors necessary to viral reproduction is not present or one detrimental to viral reproduction is present are called
Non-Permissive Cells
Refers to the number of virions that are added per cell during infection
MOI (Multiplicity of Infection)
In the One-Step Virus Growth Curve, the number of infectious virions released per average cell is called:
Burst size
During this phase no extracellular virions are detected
Latent period
Referring to a phase: The time from uncoating to just prior to the release of the first extracellular virions
Latent period
A culture is inoculated with virions, but no infectious virus can be detected during this phase___________.
Eclipse period
Time interval between uncoating [“disappearance” of viruses] and appearance, intracellularly, of first infectious progeny virions
Eclipse period
During this phase, the titer of free virus in the medium declines.
Adsorption
During this period, virus attaches to and enters cells
Adsorption
A.
B.
C.
A. Adsorption
B. Eclipse
C. Burst
The 6 steps in Virus Replication
- Attachment
- Penetration
- Uncoating
- Synthesis
- Assembly and Maturation
- Release
What are the 4 methods of viral penetration?
- Endocytosis
- Surface Fusion
- Pore-Mediated
- Antibody-Mediated
A process in which a substance gains entry into a cell without passing through the cell membrane
Endocytosis
What are the 3 kinds of endocytosis
- Phagocytosis
- Pinocytosis
- Receptor-mediated endocytosis
A process by which cells absorb metabolites, hormones, other proteins that can be hijacked by a virus
Clathrin-mediated endocytosis
The increase in local concentration of adaptor proteins on the inside face of the cell membrane allows clathrin to multimerize to form:
Clathrin-Coated Pits (CCP)
Membrane scission proteins which pinch off the Clathrin-Coated Pits are called
Dynamin
what is an endosomal signal which causes the release of enveloped viruses?
Low pH
Some non-enveloped viruses induce __________of host endosomal membrane to allow virus capsid penetration into the cytoplasm
local permeabilization
What are other types of Receptor-Mediated Endocytosis for which we don’t know the mechanism? “Jus remember the names”
- Caveolin-mediated endocytosis of virus by host. (specialized lipid rafts)
- Clathrin- and caveolin-independent endocytosis of virus by host
Some non-enveloped viruses inject their genome into the host cytoplasm. What structure is used?
Pore
