Viro5

Question 1

In this separation technique, Sample is layered as a narrow zone on the top of a density gradient

Answer 1

Rate-Zonal Centrifugation

Question 2

In this separation technique, particles are separated solely on the basis of their buoyant density

Answer 2

Isopycnic Centrifugation

Question 3

By this method, the particles will never sediment to the bottom of the tube, no matter how long the centrifugation time

Answer 3

Isopycnic Centrifugation

Question 4

Starting with a uniform mixture of sample and density gradient under centrifugal force, particles move until their density is the same as the surrounding medium. What is this point called?

Answer 4

Isopycnic Point

Question 5

A. when an object will neither sink nor float the object has…

B. and, relative to the fluid,

Answer 5

A. the same density as the fluid

B. it will remain submerged, but it will neither sink nor float

Question 6

Suitable Density Gradient Mediums for separation of viruses are:

Answer 6

Sucrose
Cesium chloride

Question 7

In this separation technique, The macroporous structure of a membrane allows large viruses to enter it and to bind to the inner pore surface easily

Answer 7

Membrane Chromatography

Question 8

With this separation method, the viruses binding to a membrane depends on the charge distribution of
the virus.

Answer 8

Ion Exchange Membrane Chromatography

Question 9

In Membrane Chromatography, larger viruses are absorbed into the substrate allowing the smaller impurities to pass

T or F

Answer 9

False

The particles are adsorbed in both ion and membrane chromatography.