Exam1

Question 1

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Answer 1

The circular tissue is rings of trachea between the cartilage. You view the cilia

Question 2

Cells which do not require attachment for growth or do not attach to the surface of the tube use what kind of culture?

Answer 2

Suspension culture

Question 3

What kind of culture are you using when the entire vessel is covered by a layer of cells?

Answer 3

Monolayer Culture

Question 4

Cells dissociated directly from the parental tissue (such as kidney or liver) of human or animal origin are used for this type of culture

Answer 4

Primary Cell Culture

Question 5

Cells in this culture have the same chromosomes and same number of chromosomes as the original tissue.

Answer 5

Primary Cell Culture

Question 6

How are cells dissociated in Primary Cell Cultures

Answer 6

trypsin

Question 7

Culture type used in producing Viral Vaccines

Answer 7

Primary Culture

Question 8

refers to the transfer of cells from one culture vessel to another culture vessel

Answer 8

Sub Culture

Question 9

What reagent is used to detach a primary cell line from a culture vessel?

Answer 9

Trypsin

Question 10

The cell lines which have a limited life span and go through a limited number of cell generations

Answer 10

Finite/Diploid Cell Lines

Question 11

These cell lines acquire the ability to divide indefinitely

Answer 11

Continuous Cell Lines

Question 12

Cell culture with limited life span: May be sub-cultured up to 100 times before the cells die

Answer 12

Finite cell lines/Diploid Cell Lines

Question 13

Culture which is Derived mainly from embryos; or from secondary cell cultures

Answer 13

Finite cell lines/Diploid Cell Lines

Question 14

Cell in this culture retain original morphology and diploid chromosome number

Answer 14

Finite cell lines/Diploid Cell Lines

Question 15

These culture cell lines exhibit the property of contact inhibition, density limitation and anchorage dependence

Answer 15

Finite cell lines/Diploid Cell Lines

Question 16

________is a diploid human cell culture line composed of fibroblasts derived from lung tissue of a three month old white female fetus

Answer 16

WI-38 (WI: Wistar Institute)

Question 17

Cell culture derived directly from cancer cells; or induced transformation of a primary or diploid cell strain to divide indefinitely

Answer 17

Continuous cell lines/Immortal Cell Lines/Heteroploid Cell Lines

Question 18

Cell Culture type that exhibits absence of contact inhibition and anchorage dependence

Answer 18

Continuous cell lines/Immortal Cell Lines/Heteroploid Cell Lines

Question 19

Cell culture line which exhibits rapid growth rate doubling time can be 12-24 hours

Answer 19

Continuous cell lines/Immortal Cell Lines/Heteroploid Cell Lines

Question 20

FDA prohibits their use in Vaccine Production

Answer 20

Continuous cell lines/Immortal Cell Lines/Heteroploid Cell Lines

Question 21

Example of Continuous Cell Line

Answer 21

Henrietta Lacks, August 01, 1920-October 04, 1951.
In loving memory of a phenomenal woman, wife and mother who touched the lives of many.
Here lies Henrietta Lacks (HeLa). Her immortal cells will continue to help mankind forever.
Eternal Love and Admiration, From Your Family

Henrietta Lacks, HeLa Cells

Cells were taken without her permission

Question 22

Cells in culture can be divided into three basic categories based on their shape and appearance

In descending order

Answer 22

Fibroblast

Epithelial-like

Lymphoblast-like

Question 23

Example of most common Cell Culture Media

Answer 23

Eagle’s Basal Medium

Question 24

Provides all the necessary nutrients (amino acids, inorganic salts, Vitamins and Glucose) required for growth of Cells

Answer 24

Culture Medium

Question 25

Leibovitz L-15 Medium is an example of what

Answer 25

Culture Media

Question 26

Why use Fetal Bovine serum in culture meida

Answer 26

FACTORS

• adhesion
• attachment
• spreading
• growth

nutrients (low molecular weight)

permeability regulation

hormones

Question 27

What percentage of FBS would be used for growth media? For maintenance

Answer 27

5-10%

0-2%

Question 28

What happened?

Answer 28

The Phenol Red in the first beaker indicates a pH above 7. The yellow beaker indicates the pH is below 7, and the viruses are dead. Start over

Question 29

Usually, buffering of culture is achieved by including an organic (e.g., HEPES) or bicarbonate based buffer. What else is also used to control pH?

Answer 29

An exogenous CO₂ atmosphere

Question 30

What can be used to prevent contamination with bacteria, mycoplasma, yeast, molds, etc

Answer 30

Antibiotics such as gentamycin

Question 31

What is this?

Answer 31

Leighton Tube

Question 32

________________refers to damage or morphological changes to host cells during virus invasion

Answer 32

Cytopathic effect or cytopathogenic effect (abbreviated CPE)

Question 33

What is being shown here?

Answer 33

Cytopathic effect

or

cytopathogenic effect

(abbreviated CPE)

Question 34

What culture type could you use for the detection of many viruses, in particular those of the respiratory group that includes Influenza A and B, RSV, Adenovirus, and Parainfluenza viruses 1,2, and 3.

Answer 34

Fluorescein stained Co-Cultured Cells

Question 35

What type of eggs are used for cultivation of Viruses in Eggs

Answer 35

Specific-Pathogen-Free (SPF) Eggs

Question 36

Routes of Egg Inoculation

Answer 36

A. Chorioallantoic membrane

B. Amnionic

C. Allantoic

D. Yolk sac

Question 37

What are these structures found after inoculating an egg with a virus?

Answer 37

Pocks on Chorioallantoic Membrane (CAM)

Question 38

What type of Laboratory Animal Inoculation?

Answer 38

A. Intracerebral Mouse Inoculation
B. Intraperitoneal Mouse Inoculation