Exam1 Flashcards
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The circular tissue is rings of trachea between the cartilage. You view the cilia
Cells which do not require attachment for growth or do not attach to the surface of the tube use what kind of culture?
Suspension culture
What kind of culture are you using when the entire vessel is covered by a layer of cells?
Monolayer Culture
Cells dissociated directly from the parental tissue (such as kidney or liver) of human or animal origin are used for this type of culture
Primary Cell Culture
Cells in this culture have the same chromosomes and same number of chromosomes as the original tissue.
Primary Cell Culture
How are cells dissociated in Primary Cell Cultures
trypsin
Culture type used in producing Viral Vaccines
Primary Culture
refers to the transfer of cells from one culture vessel to another culture vessel
Sub Culture
What reagent is used to detach a primary cell line from a culture vessel?
Trypsin
The cell lines which have a limited life span and go through a limited number of cell generations
Finite/Diploid Cell Lines
These cell lines acquire the ability to divide indefinitely
Continuous Cell Lines
Cell culture with limited life span: May be sub-cultured up to 100 times before the cells die
Finite cell lines/Diploid Cell Lines
Culture which is Derived mainly from embryos; or from secondary cell cultures
Finite cell lines/Diploid Cell Lines
Cell in this culture retain original morphology and diploid chromosome number
Finite cell lines/Diploid Cell Lines
These culture cell lines exhibit the property of contact inhibition, density limitation and anchorage dependence
Finite cell lines/Diploid Cell Lines
________is a diploid human cell culture line composed of fibroblasts derived from lung tissue of a three month old white female fetus
WI-38 (WI: Wistar Institute)
Cell culture derived directly from cancer cells; or induced transformation of a primary or diploid cell strain to divide indefinitely
Continuous cell lines/Immortal Cell Lines/Heteroploid Cell Lines
Cell Culture type that exhibits absence of contact inhibition and anchorage dependence
Continuous cell lines/Immortal Cell Lines/Heteroploid Cell Lines
Cell culture line which exhibits rapid growth rate doubling time can be 12-24 hours
Continuous cell lines/Immortal Cell Lines/Heteroploid Cell Lines
FDA prohibits their use in Vaccine Production
Continuous cell lines/Immortal Cell Lines/Heteroploid Cell Lines
Example of Continuous Cell Line
Henrietta Lacks, August 01, 1920-October 04, 1951.
In loving memory of a phenomenal woman, wife and mother who touched the lives of many.
Here lies Henrietta Lacks (HeLa). Her immortal cells will continue to help mankind forever.
Eternal Love and Admiration, From Your Family
Henrietta Lacks, HeLa Cells
Cells were taken without her permission
Cells in culture can be divided into three basic categories based on their shape and appearance
In descending order
Fibroblast
Epithelial-like
Lymphoblast-like
Example of most common Cell Culture Media
Eagle’s Basal Medium
Provides all the necessary nutrients (amino acids, inorganic salts, Vitamins and Glucose) required for growth of Cells
Culture Medium
