Viral Pathogens of the reproductive organs

Question 1

Herpes Simplex Virus Family

Answer 1

Herpesviridae

Question 2

The latency of HSV is associated with what cells?

Answer 2

Ganglion Cells or nerves

Question 3

_______ allows access of HSV from the external environment to the cell body

Answer 3

Retrograde axonal transfer

Question 4

Which subfamily of HSV has relatively rapid replication time and neuronal latency?

Answer 4

Alpha subfamily

Question 5

Heparan sulfate is a component of _______

Answer 5

basement membranes and extracellular matrix

Question 6

Above the hips: HSV-___
Below the hips: HSV-____

Answer 6

HSV-1
HSV-2

Question 7

HSV infect and replicated in what cells?

Answer 7

muco-epithelial cells

Question 8

HSV genome exists as ____ in host nucleus

Answer 8

episome

Question 9

TRUE OR FALSE: There are antivirals for HSV in its latent state

Answer 9

False, since it is inside the nucleus

Question 10

Anterograde Axonal Transport

Answer 10

Cell body to axon tip

Question 11

Retrograde axonal transport

Answer 11

Axon tip to cell body

Question 12

Immunity mechanisms for HSV

Answer 12

• Neutralizing antibodies
• Antibody-dependent cell cytotoxicity
• Cytotoxic/Killer T-cells

Question 13

Immunity through evasion for HSV

Answer 13

• IE protein
• Apoptosis inhibition

Question 14

Neonatal herpes is usually transmitted via delivery from mother with _______

Answer 14

HSV-2 Infection

Question 15

What is the Tzanck test

Answer 15

intranuclear giant cells stained by Giemsa
stain or Papanicolaou method (Pap smear)

Question 16

Diagnostic Tests for HSV

Answer 16

• Viral Culture
• Tzanck Test
• Antigen test
• HSV DNA PCR
• Antibody test

Question 17

Viral culture of HSV is usually done for

Answer 17

for epidemiologic investigations

Question 18

Treatment for HSV

Answer 18

• Acyclovir
• Foscarnet
• Valacyclovir
• Famciclovir

Question 19

Family of HIV

Answer 19

Retroviridiae

Question 20

Retroviridae has ____ which enables dsDNA to be produced from RNA template

Answer 20

Reverse transcriptase

Question 21

Subclasses of Retroviridae and describe

Answer 21

1. Oncoretroviruses: “tumor”, transforms cells
2. Lentiviruses: “slow”, persists in clinically latent state

Question 22

Susceptible Host cells of HIV

Answer 22

○ CD4+Tlymphocytes
○ Monocytes/macrophages
○ Langerhans cells (skin macrophages)
○ Dendritic cells
○ Microglia/glial cells (brain macrophages)

Question 23

The absence of ____ means a natural immunity to HIV

Answer 23

CCR5

Question 24

Definitive diagnosis of HIV

Answer 24

Western Blot

Question 25

CCR5 and gp41 inhibitors: blocks entry RT, IN, and protease inhibitors: _______

Answer 25

enzyme inhibitors

Question 26

The preferred treatment for HIV due to its virus specificity as well as decreased toxicity

Answer 26

RT, IN, and protease inhibitors

Question 27

The goal of ART is to make viral load _____

Answer 27

undetectable

Question 28

In HPV, what is the major capsid protein A. L1 B. L2

Answer 28

L1

Question 29

HPV serotype with high risk of cancer

Answer 29

HPV 16, 18, 31, 33, 35, 39, 45, 52, 56, 58, 59, 66, 68 Nakabold sa trans: 16, 18

Question 30

Diagnosis for HPV

Answer 30

- Pap spear - Antigen immunoassay - In situ nucleic acid hybridization - HPV DNA PCR