Viral

Question 1

Rinderpest

Answer 1

CD150 (SLAM) B-L membrane

Question 2

Bluetongue

Answer 2

Attachment proteins VP2, VP5

Question 3

Contagious ecthyma

Answer 3

F1L attachment protein

Question 4

Vesicular stomatitis

Answer 4

Glycoprotein G for attachment

Question 5

Foot and Mouth

Answer 5

VP1-4 attaches to alpha integrins (VB1, VB3, VB6)

Question 6

Swine Vesicular Dz and Vesicular Exanthema of Swine

Answer 6

VP1-4 attach to ICAM

Question 7

IBR (bovine herpes-1) (similar for Fe viral rhinotracheitis and Fe calicivirus)

Answer 7

Glycoproteins B, C, D bind to herpesvirus entry mediators A, C, B(nectin 1, nectin 2) and heparin sulfate
Encodes viral proteins ICPO and ICP27 that block IFN-B expression

Question 8

K9 adenovirus I

Answer 8

Fiber protein serves as virus capsid protein for attachment

Question 9

K9 Distemper

Answer 9

Attachment: Viral H protein
Fusion: Viral F protein (also leads to syncytia)
Binds to CD150 (SLAM)

Question 10

Rabies

Answer 10

Binds to envelope G proteins on myocytes

Question 11

EEE, WEE, VEE, WNV

Answer 11

Glycoproteins E1, E2

Question 12

EBV gammaherpes

Answer 12

Binds CD21 in B cells; LMP1 mimicks CD40 is exposed on B cell -> B cell proliferation

Question 13

Feline coronavirus

Answer 13

Serotype I: DC-SIGN and not APN is involved in entry process of serotype I FCoV in Monocytes
Serotype II: BOTH DC-SIGN AND APN; binding is mediated by APN and DC-SIGN is either internalization or another step
Intact 3C protein (for non-mutated FCoV) needed for sustainable infection of enterocytes and infectivity to other cats

Question 14

BRSV (paramyxovirus)

Answer 14

Attachment protein (G)
Fusion protein (F)
Small hydrophobic protein
NS1, NS2: mediate resistance to IFN
recruites WC1.1 and WC1 negative gamma/delta T cells

Question 15

Swine influenza type A

Answer 15

Hemaglutinin (HA): attachment, internalization, and intracellular fusion of virus
Neuraminidase (NA): prevents aggregation of viral progeny upon release
Polymerase (PA, PB-1, and PB-2): directs cell processes towards replication PB-2 is MOST important, takes pieces of host pre-mRNA; polymerases repsonsible for cross-species infxn
NS1: only in host cell cytosolo, interferes with host response

Question 16

BVDV

Answer 16

Erns protein is expressed in the development of PI calves

Question 17

Malignant Catarrhal Fever

Answer 17

15-A antigen epitope defines MCF

ORF25 transcripts are an index of lytic viral gene expression

Question 18

PRRS

Answer 18

Sialoadhesins

CD163

Question 19

Canine parainfluenza

Answer 19

Targets ciliated mucosal cells
Enters via HN and F glycoproteins
Bordetella most often follows

Question 20

Ovine progressive pneumonia (lentivirus) or CAEV

Answer 20

targets cells that express small ruminant lentivirus receptors A or B
Virus infects cells of monocyte macrophage system but ONLY replicates in activated MO in tissues

Question 21

Classical Swine Fever (Hog Cholera, Pestivirus)

Answer 21

Erns and E2 envelope glycoproteins involved in binding to tonsillar mucosa (note these are the same as BVDV, another pestivirus)

Question 22

African Swine Fever (asfivirus)

Answer 22

Glycoproteins p12, p54, p30 for attachment and binding to host cells

Question 23

African Horse Sickness (orbivirus)

Answer 23

VP2 and VP5 (similar to Bluetongue, another orbivirus)

NS3, a viral protein inserted into host cell membrane is a viroporin (alters permeability of cell)

Question 24

Equine Infectious Anemia (EIA virus)

Answer 24

Virus express glycoprotein gp90 that binds to equine lentivirus receptor-1 on Mono and MO
Free virus attaches to RBCs->phagocytosis and complement->anemia from IV and EV hemolysis

Question 25

Bovine Leukosis Virus (deltaretrovirus)

Answer 25

Malignant transformation of B cells Virus uses BLV envelope glycoproteins (gp51, gp30) to enter naive B cell that express BLV-binding receptor (those expressing IgM, CD5, CD11b are MORE susceptible) GENE TRANSACTIVATION integrates viral genome into B cell DNA Genes of BLV express BLX tax protein (p34tax) that stimulates B cell proliferation

Question 26

FeLV

Answer 26

B cells spread virus while T cells are primary target Four subgroups: FeLV-A spread through saliva->immunosuppression; FeTHTR1 receptor on T cells FeLV-B: neoplastic transformation; FePit1 or 2 receptor FeLV-C: anemia; FeLVCR receptor FeLV-T: infects T cells; FePit1 for receptor and FeLIX secreted by T cells determines tropism FOCMA antigen is expressed on TRANSFORMED cells ONLY

Question 27

FIV (lentivirus)

Answer 27

Target cells express CD134 receptor and CXCR4 cofactor

Question 28

PCV-2

Answer 28

Attaches to glycosaminoglycan receptors, heparin sulfate, and chondroitin sulfate B on MO, DC, LO

Question 29

Papillomavirus

Answer 29

Capsid proteins (L1 major and L2 minor)