Vibrio, Aero, Campy, Heli, Haemo

Question 1

atmospheric requirement and biochemical characteristic of vibrio

Answer 1

• facultatively anaerobic
• oxidase positive
• ferment carbohydrates
• reduces nitrates to nitrites

Question 2

vibrio ferments carbohydrates specifically?

Answer 2

sucrose

Question 3

vibrio motility

Answer 3

darting motility

Question 4

vibrio that causes the most severe disease

Answer 4

V. vulnificus

Question 5

it is usually associated with consumption of raw oysters or oyster-related injury

Answer 5

V. vulnificus

Question 6

V. parahaemolyticus was known to produce?

Answer 6

Kanagawa toxin

Question 7

vibrio that is found in contaminated fish and shellfish

Answer 7

V. parahaemolyticus

Question 8

associated with the O1 strain - epidemic cholera

Answer 8

V. cholerae

Question 9

Non-O1 strain is known to cause?

Answer 9

self-limited gastroenteritis

Question 10

vibrio that produce two types of hemolysins and a heat stable enterotoxin

Answer 10

V. cholerae

Question 11

stool specimens suspected of containing vibrio spp. should be collected and transported only in ___

Answer 11

Cary-blair medium

Question 12

medium that is toxic for vibrio and should not be used

Answer 12

buffered glycerol saline

Question 13

vibrio spp,, requires media containing NaCl except __

Answer 13

V. cholerae and V. mimicus

Question 14

pH of TCBS

Answer 14

8.6 - selective differential

Question 15

this medium may be used prior to subculture to solid medium to enhance of vibrio spp., from stool

Answer 15

alkaline peptone water

Question 16

pH of alkaline peptone water

Answer 16

8.4

Question 17

ONPG result of vibrio cholerae

Answer 17

ONPG +

Question 18

color of colonies for V. parahemolyticus

Answer 18

pale blue-green

Question 19

v. parahaemolyticus ferments?

Answer 19

glucose, maltose, mannitol

Question 20

this organism has been associated with wound infections and gastroenteritis

Answer 20

v. parahaemolyticus

Question 21

string test is a drop of?

Answer 21

0.5% aqueous solution of sodium deoxycholate

Question 22

string test positive result

Answer 22

“mucoid string”

Question 23

vibrio that grows without NaCl

Answer 23

V. cholerae
V. mimicus

Question 24

vibrio that cannot grow with the presence of 8% NaCl

Answer 24

cholerae and vulnificus

Question 25

the only vibrio that ferments lactose

Answer 25

v. vulnificus

Question 26

biochemical characteristics of pseudomonas

Answer 26

- oxidase (-) and catalase (+) - reduce nitrates to nitrites

Question 27

this form acids from carbohydrates by respiratory and fermentative metabolism

Answer 27

pseudomonas

Question 28

pseudomonas has ____ colony appearance

Answer 28

greenish, ground glass appearance

Question 29

pseudomonas has __ odor

Answer 29

fruity odor

Question 30

hemolytic pattern of chromobacterium

Answer 30

beta-hemolytic

Question 31

chromobacterium odor

Answer 31

almond-like odor

Question 32

this ethanol soluble violet produced by the chromobacterium

Answer 32

violacein

Question 33

most common cause of bacterial enteritis in the US

Answer 33

C. jejuni

Question 34

MOT of C. jejuni

Answer 34

ingestion of improperly cooked foods usually poultry products

Question 35

c. jejuni is the most recognized antecedent cause of?

Answer 35

Guillain-Barre syndrome

Question 36

the major habitat of this pathogen is the intestine of sheep and cattle

Answer 36

C. fetus subsp. fetus

Question 37

enumerate the media that can be used for Campylobacter

Answer 37

- charcoal-ceroperazone-deoxycholate agar - charcoal-based selective medium - semisolid blood-free motility medium - skirrow's medium - campylobacter agar with 5% sheep blood and five antimicrobials

Question 38

enumerate the antimicrobials found in campylobacter agar

Answer 38

- cephalothin - trimethoprim - vancomycin - polymyxin B - amphotericin B

Question 39

motile, non-spore forming, curved (comma-shaped) or S-shaped gram negative

Answer 39

campylobacter spp.,

Question 40

atmospheric requirement of campylobacter

Answer 40

5-10% oxygen

Question 41

incubation of plates at 42C increases selectivity of __

Answer 41

c. jejuni

Question 42

describe the colonies of campylobacter

Answer 42

gray, flat, irregular, spready colonies, which may become round, convex, and glistening appearance

Question 43

campylobacter that can grow in 25C temp but cannot grow in 42C

Answer 43

c. fetus

Question 44

campy that is positive for hippurate hydrolysis

Answer 44

c. jejuni

Question 45

campy resistant to nalidixic acid

Answer 45

c. fetus

Question 46

campy susceptible to cephalothin

Answer 46

c. fetus

Question 47

string test is for what organism?

Answer 47

Aeromonas

Question 48

indistinguishable from yersinia in CIN

Answer 48

aeromonas

Question 49

an autoimmune disease that targets peripheral nerves that causes paralysis

Answer 49

Guillain-Barre syndrome

Question 50

important medium for aeromonas

Answer 50

skirrow's agar

Question 51

campylobacter motility

Answer 51

cork screw motility

Question 52

haemophilus grow only on __ not on BAP

Answer 52

CHOC Agar

Question 53

h. ducreyi often have __ arrangement

Answer 53

school of fish arrangement

Question 54

streaking with hemolytic bacteria

Answer 54

satelliting phenomenon

Question 55

organism that cause infection of traumatically acquired wounds or septicemia in patients who are immunocompromised

Answer 55

aeromonas