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Bacte - lab > Vibrio, Aero, Campy, Heli, Haemo > Flashcards

Vibrio, Aero, Campy, Heli, Haemo Flashcards

1
Q

atmospheric requirement and biochemical characteristic of vibrio

A
  • facultatively anaerobic
  • oxidase positive
  • ferment carbohydrates
  • reduces nitrates to nitrites
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2
Q

vibrio ferments carbohydrates specifically?

A

sucrose

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3
Q

vibrio motility

A

darting motility

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4
Q

vibrio that causes the most severe disease

A

V. vulnificus

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5
Q

it is usually associated with consumption of raw oysters or oyster-related injury

A

V. vulnificus

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6
Q

V. parahaemolyticus was known to produce?

A

Kanagawa toxin

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7
Q

vibrio that is found in contaminated fish and shellfish

A

V. parahaemolyticus

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8
Q

associated with the O1 strain - epidemic cholera

A

V. cholerae

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9
Q

Non-O1 strain is known to cause?

A

self-limited gastroenteritis

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10
Q

vibrio that produce two types of hemolysins and a heat stable enterotoxin

A

V. cholerae

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11
Q

stool specimens suspected of containing vibrio spp. should be collected and transported only in ___

A

Cary-blair medium

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12
Q

medium that is toxic for vibrio and should not be used

A

buffered glycerol saline

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13
Q

vibrio spp,, requires media containing NaCl except __

A

V. cholerae and V. mimicus

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14
Q

pH of TCBS

A

8.6 - selective differential

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15
Q

this medium may be used prior to subculture to solid medium to enhance of vibrio spp., from stool

A

alkaline peptone water

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16
Q

pH of alkaline peptone water

A

8.4

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17
Q

ONPG result of vibrio cholerae

A

ONPG +

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18
Q

color of colonies for V. parahemolyticus

A

pale blue-green

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19
Q

v. parahaemolyticus ferments?

A

glucose, maltose, mannitol

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20
Q

this organism has been associated with wound infections and gastroenteritis

A

v. parahaemolyticus

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21
Q

string test is a drop of?

A

0.5% aqueous solution of sodium deoxycholate

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22
Q

string test positive result

A

“mucoid string”

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23
Q

vibrio that grows without NaCl

A

V. cholerae
V. mimicus

24
Q

vibrio that cannot grow with the presence of 8% NaCl

A

cholerae and vulnificus

25
Q

the only vibrio that ferments lactose

A

v. vulnificus

26
Q

biochemical characteristics of pseudomonas

A
  • oxidase (-) and catalase (+)
  • reduce nitrates to nitrites
27
Q

this form acids from carbohydrates by respiratory and fermentative metabolism

A

pseudomonas

28
Q

pseudomonas has ____ colony appearance

A

greenish, ground glass appearance

29
Q

pseudomonas has __ odor

A

fruity odor

30
Q

hemolytic pattern of chromobacterium

A

beta-hemolytic

31
Q

chromobacterium odor

A

almond-like odor

32
Q

this ethanol soluble violet produced by the chromobacterium

A

violacein

33
Q

most common cause of bacterial enteritis in the US

A

C. jejuni

34
Q

MOT of C. jejuni

A

ingestion of improperly cooked foods usually poultry products

35
Q

c. jejuni is the most recognized antecedent cause of?

A

Guillain-Barre syndrome

36
Q

the major habitat of this pathogen is the intestine of sheep and cattle

A

C. fetus subsp. fetus

37
Q

enumerate the media that can be used for Campylobacter

A
  • charcoal-ceroperazone-deoxycholate agar
  • charcoal-based selective medium
  • semisolid blood-free motility medium
  • skirrow’s medium
  • campylobacter agar with 5% sheep blood and five antimicrobials
38
Q

enumerate the antimicrobials found in campylobacter agar

A
  • cephalothin
  • trimethoprim
  • vancomycin
  • polymyxin B
  • amphotericin B
39
Q

motile, non-spore forming, curved (comma-shaped) or S-shaped gram negative

A

campylobacter spp.,

40
Q

atmospheric requirement of campylobacter

A

5-10% oxygen

41
Q

incubation of plates at 42C increases selectivity of __

A

c. jejuni

42
Q

describe the colonies of campylobacter

A

gray, flat, irregular, spready colonies, which may become round, convex, and glistening appearance

43
Q

campylobacter that can grow in 25C temp but cannot grow in 42C

A

c. fetus

44
Q

campy that is positive for hippurate hydrolysis

A

c. jejuni

45
Q

campy resistant to nalidixic acid

A

c. fetus

46
Q

campy susceptible to cephalothin

A

c. fetus

47
Q

string test is for what organism?

A

Aeromonas

48
Q

indistinguishable from yersinia in CIN

A

aeromonas

49
Q

an autoimmune disease that targets peripheral nerves that causes paralysis

A

Guillain-Barre syndrome

50
Q

important medium for aeromonas

A

skirrow’s agar

51
Q

campylobacter motility

A

cork screw motility

52
Q

haemophilus grow only on __ not on BAP

A

CHOC Agar

53
Q

h. ducreyi often have __ arrangement

A

school of fish arrangement

54
Q

streaking with hemolytic bacteria

A

satelliting phenomenon

55
Q

organism that cause infection of traumatically acquired wounds or septicemia in patients who are immunocompromised

A

aeromonas

Bacte - lab (3 decks)

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