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Bacte - lab > Other gram negative bacilli (Legio, Pasteu, Bordet, Gard, Frans)) > Flashcards

Other gram negative bacilli (Legio, Pasteu, Bordet, Gard, Frans)) Flashcards

1
Q

non-spore forming, faintly staining, thin, gram negative bacilli

A

legionella

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2
Q

legionella are found in the environment in association with?

A

water

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3
Q

legionella is isolated on __ agar

A

BCYE

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4
Q

the agar used in legionella is supplemented with growth factors including?

A
  • l-cystine
  • ferric salt
  • ketoglutarate
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5
Q

this organism is also found in manmade facilities including air-con ducts and cooling towers, potable water; large, warm water plumbing systems, whirlpools, etc

A

legionella

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6
Q

t or f: legionella is also associated with nosocomial infection

A

true: this can be widely distributed in technical medical equipment in hospitals

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7
Q

legionella is incubated for at least __ in a __ atmosphere containing no more than ___

A

incubated for at least 5 days in a humid atmosphere containing no more than 2-5% CO2

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8
Q

biochemical characteristics of legionella and motility

A
  • weakly oxidase and catalase positive
  • gelatinase positive
  • often motile
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9
Q

enumerate the diseases associated with legionella

A
  • pneumonia (legionnaires’ disease)
  • pontiac fever
  • extrapulmonary
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10
Q

atmospheric requirement and biochemical characteristic of bordetella

A

strictly aerobic, nonfermentative, and catalase positive

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11
Q

bordetella is a minute coccobacilli requiring ___

A

nicotinic acid, cysteine, and usually methionine

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12
Q

associated with kennel cough in dogs

A

bordetella bronchiseptica

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13
Q

bordetella sp., which is an uncommon human pathogen

A

B. parapertussis

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14
Q

associated with whooping cough

A

B. pertussis

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15
Q

specimen of choice for bordetella

A

nasopharyngeal swab

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16
Q

for shipment to reference lab of bordetella, what is the requirements

A

inoculated medium should be incubated for at least 24 hrs in ambient air at 35C prior to transport

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17
Q

bordetella colonies may not be visible for how many days?

A

3-4 days

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18
Q

known appearance of B. pertussis

A

drop of mercury

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19
Q

B. parapertussis grows more rapidly and will grow on ___ and occasionally on ___ agar

A

BAP and occasionally on MAC

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20
Q

this bordetella grows well on both BAP and MAC and is the most biochemically active of the three

A

B. bronchiseptica

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21
Q

enumerate the selective media for primary isolation of B. pertussis and B. parapertussis

A
  • Bordet-gengou
  • Modified Jones-Kendrick
  • Regan-Lowe
  • Stainer-Scholte
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22
Q

a selective media which work best for recovery of B. pertussis on nasopharyngeal swabs

A

Regan-Lowe

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23
Q

only oxidase negative bordetella

A

B. parapertusssis

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24
Q

the only motile bordetella

A

B. bronchiseptica

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25
biochemical characteristics of B. pertussis
Catalase and Oxidase positive Nitrate and Urease negative
26
biochemical characteristics of B. parapertussis
Catalase and Urease positive Oxidase and Nitrite negative
27
B. parapertussis is urease positive for how many hours?
24 hrs
28
days of growth for Bordetella spp., on Regan-Lowe agar
B. bronchiseptica = 1-2 days B. parapertussis = 2-3 days B. pertussis = 3-6 days
29
known appearance of brucella
sand appearance
30
biochemical characteristics, atmospheric requirement, motility, and morphology of brucella
- Catalase, oxidase positive, urease - strictly aerobic - nonmotile and nonfermenters - small coccobacilli
31
brucella is enhance by the presence of __
5-10% CO2
32
enumerate the human pathogens brucella
B. melitensis B. abortus B. suis B. canis
33
common risk factor for brucella
consumption of imported cheese made from unpasteurized goat's milk
34
human disease of brucella is called __
- Gibraltar fever - undulant fever - Malta fever - Mediterranean fever - Bang's disease
35
enumerate: brucella spp., and their animal host
abortus - cattle melitensis - sheep or goats suis - swine canis - dogs
36
time to positive urease for brucella spp.,
abortus and mellitensis - 2 hrs suis and canis - 15 mins
37
brucella positive for CO2 and H2S
b. abortus
38
b. abortus is inhibited by?
thionine
39
brucella which is inhibited by basic fuchsin
suis and canis
40
atmospheric requirement and biochemical characteristics of pasteurella
facultatively anaerobic positive for 4: CIOONPG Catalase, Indole, Oxidase, ONPG
41
nonmotile, gram-negative bacteria that range from coccobacilli to long filamentous rods
pasteurella
42
known for bipolar staining
Pasteurella
43
commensals in the upper respiratory tracts of fowl and mammals
Pasteurella
44
pasteurella are frequently isolated from?
animal bite or scratch wounds
45
pasteurella grows on __ only.
BAP
46
pasteurella is susceptible to?
penicillin
47
small, strictly aerobic, coccoid to pleomorphic rodshaped, gram - bacillus
francisella
48
francisella requires ___ for growth
cystine or cysteine
49
francisella faint bipolar staining occurs with __
aniline dyes
50
organism found in trappers, hunters, fur and meat industry workers, agricultural workers, and lab personnel
francisella spp,,
51
francisella grows on glucose-cysteine agar supplemented ___, CHOC agar with ___ or BCYE agar
glucose-cysteine agar supplemented with 5% defibrinated rabbit blood, CHOC agar with IsoVitalX or BCYE agar
52
subcultures of francisella spp., yield primarily __ colonies appearance on CHOC agar
pinpoint colony appearance
53
biochemical characteristics of francisella spp.,
oxidase and catalase negative
54
this organism requires prolonged incubation on CHOC agar
francisella
55
this organism is negative for satellite or X and V tests
francisella spp.,
56
biochemical characteristics, motility, and morphology of gardnerella
- thin, gram variable rod or coccobacillus - calatase negative - nonmotile
57
gardnerella grows best in __ atmosphere after __ of incubation
grows best in a 5% CO2 enriched atmosphere after 48 hrs of incubation
58
gardnerella direct examination of __ for the presence of __
vaginal secretions for the presence of clue cells
59
pH requirement of gardnerella
greater than 4.5
60
gardnerella odor after addition of __ to the secretions
fishy amine odor after addition of 10% potassium hydroxide (KOH)

Bacte - lab (3 decks)

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