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Bacte - lab > Other gram negative bacilli (Legio, Pasteu, Bordet, Gard, Frans)) > Flashcards

Other gram negative bacilli (Legio, Pasteu, Bordet, Gard, Frans)) Flashcards

1
Q

non-spore forming, faintly staining, thin, gram negative bacilli

A

legionella

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2
Q

legionella are found in the environment in association with?

A

water

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3
Q

legionella is isolated on __ agar

A

BCYE

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4
Q

the agar used in legionella is supplemented with growth factors including?

A
  • l-cystine
  • ferric salt
  • ketoglutarate
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5
Q

this organism is also found in manmade facilities including air-con ducts and cooling towers, potable water; large, warm water plumbing systems, whirlpools, etc

A

legionella

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6
Q

t or f: legionella is also associated with nosocomial infection

A

true: this can be widely distributed in technical medical equipment in hospitals

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7
Q

legionella is incubated for at least __ in a __ atmosphere containing no more than ___

A

incubated for at least 5 days in a humid atmosphere containing no more than 2-5% CO2

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8
Q

biochemical characteristics of legionella and motility

A
  • weakly oxidase and catalase positive
  • gelatinase positive
  • often motile
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9
Q

enumerate the diseases associated with legionella

A
  • pneumonia (legionnaires’ disease)
  • pontiac fever
  • extrapulmonary
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10
Q

atmospheric requirement and biochemical characteristic of bordetella

A

strictly aerobic, nonfermentative, and catalase positive

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11
Q

bordetella is a minute coccobacilli requiring ___

A

nicotinic acid, cysteine, and usually methionine

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12
Q

associated with kennel cough in dogs

A

bordetella bronchiseptica

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13
Q

bordetella sp., which is an uncommon human pathogen

A

B. parapertussis

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14
Q

associated with whooping cough

A

B. pertussis

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15
Q

specimen of choice for bordetella

A

nasopharyngeal swab

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16
Q

for shipment to reference lab of bordetella, what is the requirements

A

inoculated medium should be incubated for at least 24 hrs in ambient air at 35C prior to transport

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17
Q

bordetella colonies may not be visible for how many days?

A

3-4 days

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18
Q

known appearance of B. pertussis

A

drop of mercury

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19
Q

B. parapertussis grows more rapidly and will grow on ___ and occasionally on ___ agar

A

BAP and occasionally on MAC

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20
Q

this bordetella grows well on both BAP and MAC and is the most biochemically active of the three

A

B. bronchiseptica

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21
Q

enumerate the selective media for primary isolation of B. pertussis and B. parapertussis

A
  • Bordet-gengou
  • Modified Jones-Kendrick
  • Regan-Lowe
  • Stainer-Scholte
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22
Q

a selective media which work best for recovery of B. pertussis on nasopharyngeal swabs

A

Regan-Lowe

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23
Q

only oxidase negative bordetella

A

B. parapertusssis

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24
Q

the only motile bordetella

A

B. bronchiseptica

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25
Q

biochemical characteristics of B. pertussis

A

Catalase and Oxidase positive
Nitrate and Urease negative

26
Q

biochemical characteristics of B. parapertussis

A

Catalase and Urease positive
Oxidase and Nitrite negative

27
Q

B. parapertussis is urease positive for how many hours?

A

24 hrs

28
Q

days of growth for Bordetella spp., on Regan-Lowe agar

A

B. bronchiseptica = 1-2 days
B. parapertussis = 2-3 days
B. pertussis = 3-6 days

29
Q

known appearance of brucella

A

sand appearance

30
Q

biochemical characteristics, atmospheric requirement, motility, and morphology of brucella

A
  • Catalase, oxidase positive, urease
  • strictly aerobic
  • nonmotile and nonfermenters
  • small coccobacilli
31
Q

brucella is enhance by the presence of __

A

5-10% CO2

32
Q

enumerate the human pathogens brucella

A

B. melitensis
B. abortus
B. suis
B. canis

33
Q

common risk factor for brucella

A

consumption of imported cheese made from unpasteurized goat’s milk

34
Q

human disease of brucella is called __

A
  • Gibraltar fever
  • undulant fever
  • Malta fever
  • Mediterranean fever
  • Bang’s disease
35
Q

enumerate: brucella spp., and their animal host

A

abortus - cattle
melitensis - sheep or goats
suis - swine
canis - dogs

36
Q

time to positive urease for brucella spp.,

A

abortus and mellitensis - 2 hrs
suis and canis - 15 mins

37
Q

brucella positive for CO2 and H2S

A

b. abortus

38
Q

b. abortus is inhibited by?

A

thionine

39
Q

brucella which is inhibited by basic fuchsin

A

suis and canis

40
Q

atmospheric requirement and biochemical characteristics of pasteurella

A

facultatively anaerobic
positive for 4: CIOONPG
Catalase, Indole, Oxidase, ONPG

41
Q

nonmotile, gram-negative bacteria that range from coccobacilli to long filamentous rods

A

pasteurella

42
Q

known for bipolar staining

A

Pasteurella

43
Q

commensals in the upper respiratory tracts of fowl and mammals

A

Pasteurella

44
Q

pasteurella are frequently isolated from?

A

animal bite or scratch wounds

45
Q

pasteurella grows on __ only.

A

BAP

46
Q

pasteurella is susceptible to?

A

penicillin

47
Q

small, strictly aerobic, coccoid to pleomorphic rodshaped, gram - bacillus

A

francisella

48
Q

francisella requires ___ for growth

A

cystine or cysteine

49
Q

francisella faint bipolar staining occurs with __

A

aniline dyes

50
Q

organism found in trappers, hunters, fur and meat industry workers, agricultural workers, and lab personnel

A

francisella spp,,

51
Q

francisella grows on glucose-cysteine agar supplemented ___, CHOC agar with ___ or BCYE agar

A

glucose-cysteine agar supplemented with 5% defibrinated rabbit blood, CHOC agar with IsoVitalX or BCYE agar

52
Q

subcultures of francisella spp., yield primarily __ colonies appearance on CHOC agar

A

pinpoint colony appearance

53
Q

biochemical characteristics of francisella spp.,

A

oxidase and catalase negative

54
Q

this organism requires prolonged incubation on CHOC agar

A

francisella

55
Q

this organism is negative for satellite or X and V tests

A

francisella spp.,

56
Q

biochemical characteristics, motility, and morphology of gardnerella

A
  • thin, gram variable rod or coccobacillus
  • calatase negative
  • nonmotile
57
Q

gardnerella grows best in __ atmosphere after __ of incubation

A

grows best in a 5% CO2 enriched atmosphere after 48 hrs of incubation

58
Q

gardnerella direct examination of __ for the presence of __

A

vaginal secretions for the presence of clue cells

59
Q

pH requirement of gardnerella

A

greater than 4.5

60
Q

gardnerella odor after addition of __ to the secretions

A

fishy amine odor after addition of 10% potassium hydroxide (KOH)

Bacte - lab (3 decks)

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