Vibrio

Question 1

all vibrio are halophilic except

Answer 1

Vibrio cholerae
Vibrio mimicus

(halotolerant)

Question 2

natural habitants of sea water

Answer 2

vibrios

Question 3

MOT of vibrios

Answer 3

drinking contaminated water, ingestion of contaminated seafood

Question 4

if culture is prolonged, what happens to vibrio spp?

Answer 4

straight rods

Question 5

flagella of vibrio

Answer 5

single polar

Question 6

vibrio is oxidase (+) except

Answer 6

Vibrio metschnikovii

Question 7

culture media used for vibrio spp and describe

Answer 7

1. Alkaline Peptone Water
   *enrichment media
   *pH 8.4
   *subculture to TCBS
2. Thiosulfate Citrate Bile salts Sucrose Agar
   *selective media
   *pH 8.6
   *sucrose fermenter = smooth yellow colonies on green TCBS

Question 8

if swab is used for inoculation, what do you do?

Answer 8

inoculate it inside the tube

Question 9

composition of TCBS

Answer 9

(SOSS)
Sucrose
Oxgall
Sodium cholate
Sodium citrate

Question 10

bile salt derivative

Answer 10

oxgall

Question 11

indicators of TCBS

Answer 11

bromothymol blue, thymol blue

Question 12

T or F. vibrio spp survive in acidic environments

Answer 12

F

high pH (alkaline) enhances the growth of vibrio. they die in acidic environments

Question 13

these are important for inhibiting gram (+) and lactose fermenting gram (-) bacteria

Answer 13

(HOS)
High pH
Oxgall
Sodium citrate

Question 14

enumerate the sucrose positive vibrios

Answer 14

(CAFF +)
V. cholerae
V. alginolyticus
V. fluvialis
V. furnissii

Question 15

enumerate the sucrose negative vibrios

Answer 15

(PVM -)
V. parahaemolyticus
V. vulnificus
V. mimicus

Question 16

agent for cholera

Answer 16

Vibrio cholerae

Question 17

a gastrointestinal tract infection characterized by severe diarrhea and severe dehydration

Answer 17

cholera

Question 18

common cause for cholera epidemics and pandemics

Answer 18

V. cholerae O1

Question 19

3 serogroups of V. cholerae

Answer 19

(OIH-IPJ)
Ogawa - India
Inaba - Philippines
Hikojima - Japan

Question 20

2 biotypes of V. cholerae O1 and describe

Answer 20

Classical
*past epidemics

El tor
*recent epidemics
*more aggressive

Question 21

when did the 7th cholera pandemic start?

Answer 21

1961

Question 22

V. cholerae is very similar to what bioptype?

Answer 22

V. cholerae O1 El Tor biotype

Question 23

T or F. Vibrio cholerae is pathogenic to humans only

Answer 23

T

Question 24

amount of cholerae in water and food to become infectious

Answer 24

water (>10^10 organism)
food (<10^2 - 10^4 organism)

Question 25

potent enterotoxin that causes severe diarrhea in cholera

Answer 25

choleragen *affects bowel mucosa and leads to outpouring of water and electrolytes

Question 26

consistency of stool in cholera

Answer 26

rice watery stool

Question 27

if the patient is taking this, even small amounts can lead to infections of V. cholerae

Answer 27

acid suppressors

Question 28

this subunit leads to increase in cAMP what happens after?

Answer 28

A subunit leads to hypersecretion of electrolytes and water into the lumen (20-30 L of water a day)

Question 29

enumerate the laboratory diagnosis of V. cholerae

Answer 29

(SSC SSB) 1. Specimen *stool or rectal swab (acute stage) 2. Smear *gram (-) comma shaped rods 3. Culture *peptone agar (rapid growth, BAP pH 9) *TCBS 4. String test *0.5% deoxycholate *positive = viscous string 5. Serotype *O1 and O139 6. Biochemical reaction patterns

Question 30

pH of BAP used in V. cholerae

Answer 30

pH 9

Question 31

additive in string test and its positive reaction

Answer 31

0.5% sodium deoxycholate; viscous string

Question 32

treatment for cholera

Answer 32

(WOP) Water and electrolyte replacement Oral tetracycline Perform AST

Question 33

organism that causes asiatic cholera (epidemic and pandemic cholera)

Answer 33

V. cholerae

Question 34

vibrio that causes septicemia and wound infection

Answer 34

V. vulnificus

Question 35

vibrio that causes gastroenteritis and ear infection

Answer 35

V. mimicus

Question 36

vibrio that causes wound infection and ear infection

Answer 36

V. alginolyticus (may sugat si Gino sa ears)

Question 37

vibrio that causes gastroenteritis and diarrhea

Answer 37

V. fluvialis, V. furnissii

Question 38

phenomenon and agar used in C. parahaemolyticus

Answer 38

Kanagawa phenomenon Wagatsuma agar

Question 39

medium of choice to differentiate vibrio spp

Answer 39

TCBS