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Bacte Lec (FINALS) > Vibrio > Flashcards

Vibrio Flashcards

1
Q

all vibrio are halophilic except

A

Vibrio cholerae
Vibrio mimicus

(halotolerant)

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2
Q

natural habitants of sea water

A

vibrios

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3
Q

MOT of vibrios

A

drinking contaminated water, ingestion of contaminated seafood

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4
Q

if culture is prolonged, what happens to vibrio spp?

A

straight rods

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5
Q

flagella of vibrio

A

single polar

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6
Q

vibrio is oxidase (+) except

A

Vibrio metschnikovii

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7
Q

culture media used for vibrio spp and describe

A
  1. Alkaline Peptone Water
    *enrichment media
    *pH 8.4
    *subculture to TCBS
  2. Thiosulfate Citrate Bile salts Sucrose Agar
    *selective media
    *pH 8.6
    *sucrose fermenter = smooth yellow colonies on green TCBS
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8
Q

if swab is used for inoculation, what do you do?

A

inoculate it inside the tube

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9
Q

composition of TCBS

A

(SOSS)
Sucrose
Oxgall
Sodium cholate
Sodium citrate

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10
Q

bile salt derivative

A

oxgall

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11
Q

indicators of TCBS

A

bromothymol blue, thymol blue

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12
Q

T or F. vibrio spp survive in acidic environments

A

F

high pH (alkaline) enhances the growth of vibrio. they die in acidic environments

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13
Q

these are important for inhibiting gram (+) and lactose fermenting gram (-) bacteria

A

(HOS)
High pH
Oxgall
Sodium citrate

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14
Q

enumerate the sucrose positive vibrios

A

(CAFF +)
V. cholerae
V. alginolyticus
V. fluvialis
V. furnissii

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15
Q

enumerate the sucrose negative vibrios

A

(PVM -)
V. parahaemolyticus
V. vulnificus
V. mimicus

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16
Q

agent for cholera

A

Vibrio cholerae

17
Q

a gastrointestinal tract infection characterized by severe diarrhea and severe dehydration

A

cholera

18
Q

common cause for cholera epidemics and pandemics

A

V. cholerae O1

19
Q

3 serogroups of V. cholerae

A

(OIH-IPJ)
Ogawa - India
Inaba - Philippines
Hikojima - Japan

20
Q

2 biotypes of V. cholerae O1 and describe

A

Classical
*past epidemics

El tor
*recent epidemics
*more aggressive

21
Q

when did the 7th cholera pandemic start?

A

1961

22
Q

V. cholerae is very similar to what bioptype?

A

V. cholerae O1 El Tor biotype

23
Q

T or F. Vibrio cholerae is pathogenic to humans only

A

T

24
Q

amount of cholerae in water and food to become infectious

A

water (>10^10 organism)
food (<10^2 - 10^4 organism)

25
Q

potent enterotoxin that causes severe diarrhea in cholera

A

choleragen

*affects bowel mucosa and leads to outpouring of water and electrolytes

26
Q

consistency of stool in cholera

A

rice watery stool

27
Q

if the patient is taking this, even small amounts can lead to infections of V. cholerae

A

acid suppressors

28
Q

this subunit leads to increase in cAMP

what happens after?

A

A subunit

leads to hypersecretion of electrolytes and water into the lumen (20-30 L of water a day)

29
Q

enumerate the laboratory diagnosis of V. cholerae

A

(SSC SSB)
1. Specimen
*stool or rectal swab (acute stage)

  1. Smear
    *gram (-) comma shaped rods
  2. Culture
    *peptone agar (rapid growth, BAP pH 9)
    *TCBS
  3. String test
    *0.5% deoxycholate
    *positive = viscous string
  4. Serotype
    *O1 and O139
  5. Biochemical reaction patterns
30
Q

pH of BAP used in V. cholerae

A

pH 9

31
Q

additive in string test and its positive reaction

A

0.5% sodium deoxycholate; viscous string

32
Q

treatment for cholera

A

(WOP)
Water and electrolyte replacement
Oral tetracycline
Perform AST

33
Q

organism that causes asiatic cholera (epidemic and pandemic cholera)

A

V. cholerae

34
Q

vibrio that causes septicemia and wound infection

A

V. vulnificus

35
Q

vibrio that causes gastroenteritis and ear infection

A

V. mimicus

36
Q

vibrio that causes wound infection and ear infection

A

V. alginolyticus
(may sugat si Gino sa ears)

37
Q

vibrio that causes gastroenteritis and diarrhea

A

V. fluvialis, V. furnissii

38
Q

phenomenon and agar used in C. parahaemolyticus

A

Kanagawa phenomenon

Wagatsuma agar

39
Q

medium of choice to differentiate vibrio spp

A

TCBS

Bacte Lec (FINALS) (3 decks)

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