NFO (Pseudomonas, Burkholderia, Stenotrophomonas, Acinetobacter, Alcaligenes

Question 1

fermentation occurs in the presence/absence of oxygen

Answer 1

absence of oxygen
*greater acidity, less energy

Question 2

oxidative metabolism of carbohydrates

Answer 2

non-fermentative

Question 3

GNB that do not use CHO; use other compounds as source of energy

Answer 3

non-saccharolytic or non-oxidizers

Question 4

point of colonization of non-fermenters

Answer 4

mucous membrane and skin of humans

Question 5

all NFGNB are oxidase (+) except:

Answer 5

P. luteolus, P. oryzihabitans

Question 6

GNB that can oxidize and ferment CHO

Answer 6

Vibrionaceae, Enterobacteriaceae

Question 7

pseudomonas entry to sterile sites

Answer 7

(BTW)
Burns
Trauma
Wounds

Question 8

oxygen requirement of pseudomonas

Answer 8

obligate aerobe

Question 9

preferred culture medium of non-fermenters

Answer 9

Sheep Blood Agar

Question 10

can be a sole source of carbon

Answer 10

Acetamide
*positive: blue
*negative: green

Question 11

medium and procedure in Modified Indole Test

Answer 11

medium: SIM

1. incubate 24 hours
2. add xylene
3. incubate 24 hours
4. add ehrlich reagent