Aeromonas, Plesiomonas, Campylobacter, Helicobacter

Question 1

found in both fresh and seawater

Answer 1

Aeromonas

Question 2

enumerate the laboratory diagnosis of aeromonas

Answer 2

(MESC GI)
MAC
EMB
SSA
CIN medium
*Cefsulodin Irgasan Novobiocin

Glucose fermentation
Indole production

Question 3

flagella of aeromonas

Answer 3

monotrichous

Question 4

Aeromonas spp.

Answer 4

Aeromonas hydrophilia

Question 5

enzymes produced by aeromonas

Answer 5

(PLaN)
Protease
Lipase
Nuclease

Question 6

enterotoxins of aeromonas

Answer 6

heat labile enterotoxin
heat stable cytotoxic enterotocin

Question 7

diseases associated with aeromonas

Answer 7

(MCWiGS)
Meningitis
Cellulitis
Wound infection
Gastroenteritis
Septicemia

Question 8

clinically significant Plesiomonas spp

Answer 8

Plesiomonas shigelloides

Question 9

causes gastroenteritis that is mild with no blood or mucous

Answer 9

Plesiomonas

Question 10

now categorized as enteric bacteria

Answer 10

Plesiomonas

Question 11

morphology of Plesiomonas

Answer 11

straight, round

Question 12

enumerate the laboratory diagnosis of Plesiomonas

Answer 12

(BaNME OI)
BAP
NLF on MAC
EMB

Oxidase (+)
Indole (+)

Question 13

most frequently isolated species of Campylobacter

Answer 13

C. jejuni and C. coli

*clinically indistinguishable

Question 14

enumerate the virulence factor of Campylobacter

Answer 14

(CCE)
Cytotoxin
Cytotoxic factor
Enterotoxin

Question 15

environment required by Campylobacter

Answer 15

microaerophilic or capnophilic

*5% O2
*10% CO2
*85% N

Question 16

in what organ does campylobacter multiply?

Answer 16

small intestine

Question 17

what organism causes blood cells in stool?

Answer 17

Campylobacter

Question 18

enumerate the laboratory diagnosis of Campylobacter

Answer 18

Catalase (+)
Oxidase (+)

Question 19

specimen used and optimal temperature for Campylobacter

Answer 19

stool, 42-43*C

Question 20

morphology of Campylobacter

Answer 20

(GGNS)
gull wing shaped rods,
gray to pink, yellow to gray colonies,
non hemolytic,
slightly mucoid

Question 21

culture media used for campylobacter

Answer 21

Selective Media

1. Campylobacter blood agar
2. Skirrow’s medium

Question 22

composition of campylobacter blood agar

Answer 22

(VPCAT)
Vancomycin
Polymyxin B
Cephalothin
Amphotericin B
Trimethoprim

Question 23

composition of skirrow’s medium

Answer 23

(PVT / Private Skirrow)
Polymyxin B
Vancomycin
Trimethoprim

Question 24

disorder caused by C. jejuni

Answer 24

Guillain-Barre Syndrome

Question 25

morphology of H. pylori

Answer 25

U shaped bacilli

Question 26

environment requirements of Helicobacter

Answer 26

microaerophilic *5% O2 *10% CO2 *85% N

Question 27

lab diagnosis of helicobacter

Answer 27

(CUNO) Catalase (+) Urease (+) Nitrate (-) Oxidase (+)

Question 28

natural habitat of H. pylori

Answer 28

mucous secreting stomach cells

Question 29

what pH does H. pylori grow best

Answer 29

pH 6 to 7

Question 30

causes the increase in ammonia

Answer 30

urease

Question 31

this neutralizes HCl

Answer 31

ammonia

Question 32

best to identify H. pylori

Answer 32

gastric biopsy for histologic examination

Question 33

stain used in H. pylori

Answer 33

Special silver stain Modified giemsa

Question 34

environment requirements of H. pylori

Answer 34

microaerophilic; incubate at 37*C in increased humidity

Question 35

culture media for H. pylori

Answer 35

mashed tissue on SBA

Question 36

describe the colonies of H. pylori

Answer 36

small, gray, translucent, slighty hemolytic

Question 37

detects urease activity

Answer 37

Carbon Urea Breath Test

Question 38

treatment for H. pylori

Answer 38

(TAP) Triple therapy *(MBA) *Metronidazole + Bismuth subsalicylate/subcitrate + Amoxicillin/tetracycline *14 days Acid suppressing agent *4-6 weeks Proton pump inhibitor

Question 39

implicated in gastric cancers

Answer 39

H. pylori

Question 40

common causes of bacterial diarrhea worldwide

Answer 40

C. jejuni

Question 41

gold standard of h. pylori

Answer 41

histological staining and culture of biopsy tissue from stomach or duodenum