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Bacte Lec (FINALS) > Aeromonas, Plesiomonas, Campylobacter, Helicobacter > Flashcards

Aeromonas, Plesiomonas, Campylobacter, Helicobacter Flashcards

1
Q

found in both fresh and seawater

A

Aeromonas

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2
Q

enumerate the laboratory diagnosis of aeromonas

A

(MESC GI)
MAC
EMB
SSA
CIN medium
*Cefsulodin Irgasan Novobiocin

Glucose fermentation
Indole production

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3
Q

flagella of aeromonas

A

monotrichous

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4
Q

Aeromonas spp.

A

Aeromonas hydrophilia

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5
Q

enzymes produced by aeromonas

A

(PLaN)
Protease
Lipase
Nuclease

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6
Q

enterotoxins of aeromonas

A

heat labile enterotoxin
heat stable cytotoxic enterotocin

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7
Q

diseases associated with aeromonas

A

(MCWiGS)
Meningitis
Cellulitis
Wound infection
Gastroenteritis
Septicemia

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8
Q

clinically significant Plesiomonas spp

A

Plesiomonas shigelloides

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9
Q

causes gastroenteritis that is mild with no blood or mucous

A

Plesiomonas

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10
Q

now categorized as enteric bacteria

A

Plesiomonas

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11
Q

morphology of Plesiomonas

A

straight, round

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12
Q

enumerate the laboratory diagnosis of Plesiomonas

A

(BaNME OI)
BAP
NLF on MAC
EMB

Oxidase (+)
Indole (+)

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13
Q

most frequently isolated species of Campylobacter

A

C. jejuni and C. coli

*clinically indistinguishable

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14
Q

enumerate the virulence factor of Campylobacter

A

(CCE)
Cytotoxin
Cytotoxic factor
Enterotoxin

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15
Q

environment required by Campylobacter

A

microaerophilic or capnophilic

*5% O2
*10% CO2
*85% N

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16
Q

in what organ does campylobacter multiply?

A

small intestine

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17
Q

what organism causes blood cells in stool?

A

Campylobacter

18
Q

enumerate the laboratory diagnosis of Campylobacter

A

Catalase (+)
Oxidase (+)

19
Q

specimen used and optimal temperature for Campylobacter

A

stool, 42-43*C

20
Q

morphology of Campylobacter

A

(GGNS)
gull wing shaped rods,
gray to pink, yellow to gray colonies,
non hemolytic,
slightly mucoid

21
Q

culture media used for campylobacter

A

Selective Media

  1. Campylobacter blood agar
  2. Skirrow’s medium
22
Q

composition of campylobacter blood agar

A

(VPCAT)
Vancomycin
Polymyxin B
Cephalothin
Amphotericin B
Trimethoprim

23
Q

composition of skirrow’s medium

A

(PVT / Private Skirrow)
Polymyxin B
Vancomycin
Trimethoprim

24
Q

disorder caused by C. jejuni

A

Guillain-Barre Syndrome

25
Q

morphology of H. pylori

A

U shaped bacilli

26
Q

environment requirements of Helicobacter

A

microaerophilic

*5% O2
*10% CO2
*85% N

27
Q

lab diagnosis of helicobacter

A

(CUNO)
Catalase (+)
Urease (+)
Nitrate (-)
Oxidase (+)

28
Q

natural habitat of H. pylori

A

mucous secreting stomach cells

29
Q

what pH does H. pylori grow best

A

pH 6 to 7

30
Q

causes the increase in ammonia

A

urease

31
Q

this neutralizes HCl

A

ammonia

32
Q

best to identify H. pylori

A

gastric biopsy for histologic examination

33
Q

stain used in H. pylori

A

Special silver stain
Modified giemsa

34
Q

environment requirements of H. pylori

A

microaerophilic; incubate at 37*C in increased humidity

35
Q

culture media for H. pylori

A

mashed tissue on SBA

36
Q

describe the colonies of H. pylori

A

small, gray, translucent, slighty hemolytic

37
Q

detects urease activity

A

Carbon Urea Breath Test

38
Q

treatment for H. pylori

A

(TAP)
Triple therapy
*(MBA)
*Metronidazole + Bismuth subsalicylate/subcitrate + Amoxicillin/tetracycline
*14 days

Acid suppressing agent
*4-6 weeks

Proton pump inhibitor

39
Q

implicated in gastric cancers

A

H. pylori

40
Q

common causes of bacterial diarrhea worldwide

A

C. jejuni

41
Q

gold standard of h. pylori

A

histological staining and culture of biopsy tissue from stomach or duodenum

Bacte Lec (FINALS) (3 decks)

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