VIBRIO Flashcards by FRANCHEZKA MARIE IGNACIO

Q

Test used to identify Vibrio

A

String Test

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Q

String test reagent

A

sodium deoxycholate

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Q

(R) Vibriostatic agent
(-) string Test

A

Aeromonas

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Q

Media used for Vibrio

A

TCBS - thiosulfate citrate bile salt agar

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Q

TCBS what kind of media

A

Selective differential

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Q

TCBS uses what CHO

A

Sucrose

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Q

TCBS indicator

A

Bromthymol blue
Thymol blue

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Q

TCBS colonies color

A

(+) Yellow
(-) green

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Q

Enrichment media for Vibrios

A

APW

ALKALINE PEPTONE WATER

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Q

Non-halophilic vibrios

A

Mimicus
Cholerae

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Q

Non-Sucrose Fermenters Vibrio

A

MVP

Mimicus
Vulnificus
Parahaemolyticus

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Q

Sucrose fermenters Vibrio

A

FAC-FM

Fluvialis
Alginolyticus
Cholorae
Furnissi
Metschnikovii

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Q

Most significant vibrio

A

V cholorae

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Q

Clinical significance V cholorae

A

Cholera

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Q

Stool appearance caused by V cholorae

A

Rice watery stool

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Q

2nd most common vibrio

A

V parahaemolyticus

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Q

Clinical significance of V parahaemolyticus

A

Summer diarrhea in Japan

Q

Phenomenon associated with V parahaemolyticus

A

Kanagawa Phenomenon

Q

Kanagawa phenomenon is due to

A

TDH

Thermostable Direct Hemolysin

Q

Media used for V parahaemolytics

A

Wagatsuma Agar

Q

Component of Wagatsuma agar

A

High salt mannitol

Q

Occupational hazard vibrio

A

V alginolyticus

Q

V alginolyticus is acquired from

A

Continuous contact with sea water

Q

Test to separate Vibrio from Enterobacteriaceae

A

(+) Oxidase

Q

Test to differentiate Vibrio from PLESIOMONAS

A

(+) inositol test

Q

Test to diiferentiate Vibrio from PSEUDOMONAS

A

CHO Fermentation
- glucose (+) Vibrio and Entero

Q

All Vibrio are lactose negative EXCEPT

A

V vulnificus

Q

Uses 2 media

A

Decarboxylase test

Q

Media used in Decarboxylase test

A

Moellers/LIA

Q

CHO used for Decarboxylase

A

Glucose

Q

Indicator used for Decarboxylase

A

Bromcresol purple

Q

3 Amino Acid used in Decarboxylase test

A

LOA

Lysine - remove cadaverine
Ornithine - detect putrescine
Arginine - remove Citrulline

Q

Color of Result in Decarboxylase test

A

(+) purple
(-) yellow

Q

Triple Decarboxylase (+)

A

Plesiomonas

Q

Triple decarboxylase test (-)

A

Pantoea agglomerans

Q

Motility of C.jejuni and C. Coli

A

Darting motility

Q

Oxygen requirement of C coli and C jejuni

A

Microaerophilic

Q

Special requriement of C coli and C jejuni

A

Capnophilic

Q

Shaped and Appearance of C coli and C jejuni

A

S shaped
Wings of seagull

Q

Capable of Urease production

A

Helicobacter pylori