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BACTERIOLOGY (Final) > ANAEROBES > Flashcards

ANAEROBES Flashcards

1
Q

provide anaerobic environment

A

GASPAK jar

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2
Q

Substance added to provide oxygen free environment

A

Pallidum catalyst

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3
Q

Indicators used for anaerobic bacteria

A

Rezusarin
Methylene blue

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4
Q

Emit foul odor

A

C. Difficile
Fusobacterium
Porphyromonas

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5
Q

Brick red fluorescence; black pigment

A

Porphyromonas
Prevotella

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6
Q

Vellonella

A

Red fluorescence; rapidly fade

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7
Q

Fusobacterium nucleatum

A

Chartreuse fluorescence, yellow fluorescence

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8
Q

Actinomyces, Propionibacterium, Cutibacterium, Eubacterium nodatum

A

Sulfur granules

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9
Q

Microscopic: fusobacterium nucleaticum

A

Taperred ends, toothpick appearance

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10
Q

Microscopic: Bifidobacterium

A

Bifid ends/ dog bone biscuit shaped

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11
Q

Bile tolerant, cause of aspiration pneumonia

A

Bacteroides fragilis

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12
Q

Appearance of B.fragilis microscopically

A

Pin shaped

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13
Q

B.fragilis is resistant to

A

KVC
kanamycin
Vancomycin
Colistin

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14
Q

Clinical significance of Propionibacterium

A

Anaerobic diptheroid

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15
Q

Spot indole test positive color

A

Blue/ green

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16
Q

Spot indole test (+) organism

A

Cutibacterium

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17
Q

Spot indole test (-) organism

A

Propionibacterium

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18
Q

Spot indole (-) color

A

Pink/orange

19
Q

Potency disk test is same with

A

Kirby bauer

20
Q

Purpose of Potency disk test

A

Verify gram stain of Anaerobic
Susceptibility/Resistance

21
Q

Spot indole test reagent

A

P-dimethylaminocinnamaldehyde

22
Q

PYR reagent and organism

A

P-dimethylaminocinnamaldehyde and S. Pyogenes

23
Q

Antibiotic in Potency

A

KVC
Kanamycin - 1000
Vancomycin - 5g
Colistin - 10g

24
Q

RRR

A

B. Fragilis

25
Q

SRS

A

Fusobacterium
B.ureolyticus
Veillonella

26
Q

SSR

A

Clostridium

27
Q

RSR

A

Porphyromonas
P. Anaerobius

28
Q

RRS

A

Prevotella

29
Q

SPS Sensitive test ; SPS stands for

A

Sodium Polyethanol Sulfonate

30
Q

Organism inhibited by SPS

A

P. Anaerobius

31
Q

Concentration of SPS in blood culture

A

0.025%

32
Q

To counter act inhibition of P. Anaerobius you should add

A

1% GELATIN

33
Q

SPS:

C. Tetani and C. Septicum appears

A
  • Swarming motility (like proteus)
  • spore forming
34
Q

SPS:

Actinomyces israelii appears

A
  • Molar tooth (colonies)
  • causes Lumpy jaw: Actinomycosis of Jaw
35
Q

SPS:

Anaerobic Staphylococcus

A

Peptococcus niger

36
Q

SPS:

Anaerobic streptococcus

A

Peptostreptococcus

37
Q

SPS:

Prevotella
Porphyromonas

A

Brick red fluorescence

38
Q

Prevotella is important in

A

Dental biofilm

39
Q

Flexible media

A

Thioglycolate

40
Q

Thioglycolate is used for

A

Anaerobe, Facultative anaearobe, strict aerobe

41
Q

Thioglycollate: Sign of Growth

A

Turbidity

42
Q

Thioglycolate:

Anaerobic location

A

Bottom

43
Q

Thioglycolate:

Aerobic location

A

Surfac

44
Q

Thioglycolate:

Facultative anaerobe location

A

Dispersed

BACTERIOLOGY (Final) (10 decks)

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