ANAEROBES Flashcards
provide anaerobic environment
GASPAK jar
Substance added to provide oxygen free environment
Pallidum catalyst
Indicators used for anaerobic bacteria
Rezusarin
Methylene blue
Emit foul odor
C. Difficile
Fusobacterium
Porphyromonas
Brick red fluorescence; black pigment
Porphyromonas
Prevotella
Vellonella
Red fluorescence; rapidly fade
Fusobacterium nucleatum
Chartreuse fluorescence, yellow fluorescence
Actinomyces, Propionibacterium, Cutibacterium, Eubacterium nodatum
Sulfur granules
Microscopic: fusobacterium nucleaticum
Taperred ends, toothpick appearance
Microscopic: Bifidobacterium
Bifid ends/ dog bone biscuit shaped
Bile tolerant, cause of aspiration pneumonia
Bacteroides fragilis
Appearance of B.fragilis microscopically
Pin shaped
B.fragilis is resistant to
KVC
kanamycin
Vancomycin
Colistin
Clinical significance of Propionibacterium
Anaerobic diptheroid
Spot indole test positive color
Blue/ green
Spot indole test (+) organism
Cutibacterium
Spot indole test (-) organism
Propionibacterium
Spot indole (-) color
Pink/orange
Potency disk test is same with
Kirby bauer
Purpose of Potency disk test
Verify gram stain of Anaerobic
Susceptibility/Resistance
Spot indole test reagent
P-dimethylaminocinnamaldehyde
PYR reagent and organism
P-dimethylaminocinnamaldehyde and S. Pyogenes
Antibiotic in Potency
KVC
Kanamycin - 1000
Vancomycin - 5g
Colistin - 10g
RRR
B. Fragilis
SRS
Fusobacterium
B.ureolyticus
Veillonella
SSR
Clostridium
RSR
Porphyromonas
P. Anaerobius
RRS
Prevotella
SPS Sensitive test ; SPS stands for
Sodium Polyethanol Sulfonate
Organism inhibited by SPS
P. Anaerobius
Concentration of SPS in blood culture
0.025%
To counter act inhibition of P. Anaerobius you should add
1% GELATIN
SPS:
C. Tetani and C. Septicum appears
- Swarming motility (like proteus)
- spore forming
SPS:
Actinomyces israelii appears
- Molar tooth (colonies)
- causes Lumpy jaw: Actinomycosis of Jaw
SPS:
Anaerobic Staphylococcus
Peptococcus niger
SPS:
Anaerobic streptococcus
Peptostreptococcus
SPS:
Prevotella
Porphyromonas
Brick red fluorescence
Prevotella is important in
Dental biofilm
Flexible media
Thioglycolate
Thioglycolate is used for
Anaerobe, Facultative anaearobe, strict aerobe
Thioglycollate: Sign of Growth
Turbidity
Thioglycolate:
Anaerobic location
Bottom
Thioglycolate:
Aerobic location
Surfac
Thioglycolate:
Facultative anaerobe location
Dispersed