SPIROCHETES Flashcards
Genus under spirochetes are
Leptomonas
Borellia
Treponema
Gram stain characteristics of Spirochetes
Gram (-)
Motility of spirochetes
Corkscrew motility
Corkscrew motility must consist of
Axial filaments
Periplasmic flagella
Spirochetes may be view using
Dark field microscopy
Stain used for Spirochetes
Silver stain
Borellia is viewed in
Bright filled microscopy
Borellia is also known as
Blood spirochetes
Culture medium used for Borellia
Kelly medium
Diagnostic tool for Borellia
Sero test & Giemsa stain
Species under Borellia
B. Burgdorferi
B. Reccurentis
B. Parkeri
B. Duttoni
B. Harmesii
Clinical significance of B. Burgdorferi
Lyme disease
Lyme disease is transmitted by
Ixode ticks
Black legged ticks
Deer ticks
Stage 1: Lyme disease
Bull eye’s appearance
ECM (erythrema chronicum migrans)
Stage 2: Lyme disease
ECM Accumulation throughtout the body
Stage 3: Lyme disease
Chronic arthritis
Nurologic symptoms
B. Recurrentis clinical significance
Louse Borne Relapsing Fever
Arthropod asscociated in B. Recurrentis
Pediculus
Pendiculus capitis (head)
Pendiculus corporis (body)
Arthropod associated harmessi, duttoni, parkeri
Soft ticks, Ornithodoros ticks
Leptomonas Characteristics
Tightly coiled, one or more are bent appears like a hook
Culture used for Leptomonas
Fletchers Media
microscopy used for leptomonas detection
Dark-filled
Fluorescence
Phase-contrast
Species under Leptomonas that causes animal and human LEPTOSPIROSIS
L. Interrogans
2 phase of leptospirosis
Anicteric
Icteric
Anicteric phase happens when there is
High fever, rashes, septicemia
Leptosipirosis: Icteric phase
Kidney and liver
Leptospirosis: Icteric specimen
Blood CSF - 1st week specimen
Urine - 2nd wek specimen
HALLMARK immune response of Leptos
Aseptic Meningitis
Severe form of Leptos
Weil’s disease
Gold standard for diagnosis of Leptospirosis
Microscopic Agglutination test
Spirochetes form through binary fission are
BORELLIA
LEPTOMONAS
Spirochetes formed thourgh TRANSVERSE FISSION
Treponema
Spirochetes with 4-14 coils
Treponema
Trepomonas is non-culturable in
VITRO
Substance needed to maintain live treponema
Testicle chancre of rabbit
Clinical significance of T. Pallidum
French disease, syphilis, Great pox
Primary syphilis
Genital lesion (hard/huntarian lesion)
Secondary lesion
Extragenital lesion (condyloma latum)
Latency phase of Syphilis
Absence of lesion, + blood test
Tertiary
Deep organ lesion (gumma’s)
3 subspecies of T.pallidum
T.P perteu - Yaws frambresia
T.P carateneum - Pinta
T.P endemicum - Bejel/endemicum syphihlis
T. Cuniculi clinical significance
Venereal spirochetosis
T.vincentii clinical significance
Trench mouth
Vincent’s stomatitis