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BI 338 Cells > Vesicular Traficking II (Lecture 15) > Flashcards

Vesicular Traficking II (Lecture 15) Flashcards

1
Q

The ______ pathway goes from the ER to the cell surface

A

Secretory

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2
Q

The ______ pathway goes from the cell surface back to the ER.

A

Endocytic

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3
Q

The first step in the secretory pathway is mediated by ______ vesicles.

A

COPII

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4
Q

True or False: In the secretory pathway, they vesicles contain newly synthesized proteins destined for Golgi, cell surface, or lysosomes.

A

True

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5
Q

True or False: In the secretory pathway, vesicles contain t-SNARE components.

A

False, v-SNARE

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6
Q

The secretory pathway moves in the ______ direction.

A

Anterograde

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7
Q

v-SNARE recycling and retrieval of missorted ER-resident proteins from the cis-Golgi is via ______-coated vesicles in the ______ direction

A

COPI, retrograde

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8
Q

How do formation of class B sec mutants occur in yeast cells?

A

Triggered when Sec12 catalyzes activation of cytosolic Sar1

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9
Q

What mutant class has accumulation in the cytosol and doesn’t function to transport into the ER.

A

Class A

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10
Q

What class doesn’t allow budding of vesicles from the rough ER and causes accumulation in the rough ER.

A

Class B

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11
Q

What class causes accumulation in the Golgi and inhibits transport from Golgi to secretory vesicles

A

Class D

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12
Q

What is the fate of secretory proteins and defective function for Class E

A

Accumulation in the secretory vesicles, transport from secretory cells to cell surface

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13
Q

What is the fate of secretory proteins and defective function for Class C

A

Accumulation in the ER-to-Golgi transport vesicles, fusion of transport vesicles with Golgi

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14
Q

True or False: Sec24 interaction with cytosolic domain di-acidic targeting signal (purple) recruits cargo protein into the vesicle membrane.

A

True

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15
Q

True or False: Some integral ER membrane proteins are targeted to COPII vesicles for transport to Golgi.

A

True

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16
Q

What type of sorting signal is used for protein targeting to COPII for cytosolic domains?

A

Di-acidic sorting signal

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17
Q

True or False: The di-acidic sorting signal binds to the Sec23 complex.

A

False, Sec 24 complex

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18
Q

True or False: Certain integral ER membrane proteins also bind cargo proteins in the lumen via the exoplasmic domain —

A

True

19
Q

______ acts as a cargo receptor for collagen; simultaneously binds collagen and ______ on opposite sides of the membrane.

A

TANGO1, Sec24

20
Q

True or False: Other sorting signals exist but can bind to either Sec24, Sec23, and Sar1.

A

False, only Sec24

21
Q

True or False: Many vesicles move by diffusion for small distances.

A

True

22
Q

How do vesicles travel long distances?

A

Use microtubules as railroad tracks

23
Q

True or False: In ER - resident proteins, many of the soluble proteins assist in folding and modification of newly synthesized secretory proteins.

A

True

24
Q

What are 2 examples of soluble proteins that assit in folding modification?

A

chaperone BiP, protein disulfide isomerase

25
Q

What happens when soluble proteins accidently enter COPII vesicles?

A

COPI recycles these back so they do not become depleted

26
Q

True or False: Most soluble proteins carry a KDEL sequence at the N terminus.

A

False, C-terminus

27
Q

What does KDEL stand for?

A

Lys-Asp-Glu-Leu

28
Q

What is KDEL necessary for?

A

localize a protein to the ER

29
Q

True or False: KDEL receptor acts mainly to retrieve soluble proteins containing the KDEL sorting signal

A

True

30
Q

True or False: The KDEL receptor binds at high pH.

A

False, low pH

31
Q

True or False: The KDEL receptor is found mainly in the ER.

A

False, Found mainly in transport vesicles

32
Q

True or False: In the KDEL receptor, the differing pH in Golgi vs ER mediates peptide binding or release.

A

True

33
Q

KDEL receptor contains _____ sorting signal at the very end of the C-terminal segment (cytosolic).

A

KKXX (X stands for any amino acid)

34
Q

True or False: KKXX signal binds to a complex of COPII subunits to incorporate membrane proteins into COPII vesicles.

A

True

35
Q

Other the the KDEL receptor, what other sequence can target certain proteins to COPI vesicles?

A

Di-arginine

36
Q

True or False: The DI-arginine sequence does not have to occur at the C terminus.

A

True

37
Q

Cisternal maturation is responsible for ______ transport.

A

Anterograde

38
Q

Vesicle-based transport visible by electron microscopy are all ______ transporters.

A

COPI (retrograde)

39
Q

How does retrograde transport effect the makeup of the Cisternal lumen?

A

medial-Golgi is gaining trans-Golgi enzymes, and losing medial-Golgi enzymes to the cis-Golgi

therefore, medial-Golgi thus progressively becomes the new trans-Golgi compartment, through cisternal maturation

40
Q

______ -coated vesicles transport newly synthesized proteins containing Golgitargeting sequences in their cytosolic domain or bound to such proteins from the rough ER to the cis-Golgi (anterograde direction)

A

COPII

41
Q

______-coated vesicles transport vesicles carrying ER/Golgi-resident proteins in the retrograde direction, which supports Golgi cisternal maturation

A

COPI

42
Q

______-coated vesicles transport proteins from the plasma membrane (cell surface) and thetrans-Golgi network to late endosomes

A

Clathrin

43
Q

What GTP-binding protein is in COPI, COPII, and Clathrin coated proteins?

A

ARF protein in COPI and clathrin-coated vesicles — 

Sar1 protein in COPII vesicles

BI 338 Cells (21 decks)

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