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Molecular Biology > Vesicular Trafficking I > Flashcards

Vesicular Trafficking I Flashcards

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1
Q

In regards to vesicular transport, vesicles __ off from primary compartment and ___ with the next one.

A

bud; fuse

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2
Q

contents of vesicle is called what

A

cargo

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3
Q

Is vesicular transport directional?

A

yes

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4
Q

vesicular transport mediates a ___ exchange of components between various membrane enclosed compartments

A

continous

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5
Q

composition of the membrane and ___ ___ on membrane surface provide cues for incoming traffic

A

molecular markers

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6
Q

combination of markers gives each compartment what?

A

its full address

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7
Q

retrieval pathways do what?

A

return molecules to source

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8
Q

Cells segregate proteins into membrane domains by assembling a special __ on cytosolic side of the membrane

A

coat

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9
Q

When transport vesicles bud off, are they coated or not?

A

coated

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10
Q

What are the 2 functions of the coat on vesicles?

A

concentrates select proteins in a specialized patch for transport; coat molds the vesicle to form a basket like lattice that deforms the membrane and gives shape to the vesicle

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11
Q

When is the vesicular coat discarded?

A

before vesicle fuses with target membrane

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12
Q

COPI and COPII are coats that mediate transport between what two cellular locations?

A

ER and Golgi

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13
Q

Clathrin-coated vesicles mediate transport from what two cellular location?

A

Golgi and plasma membrane

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14
Q

Which coated vesicle returns to ER from Golgi?

A

COPI

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15
Q

Which coated vesicle travels from ER to Golgi?

A

COPII

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16
Q

Describe the structure of clathrin

A

3 large and 3 small polypeptide chains form a triskelion

17
Q

Triskelions assemble into what?

A

basket-like structures that form coated pits on cytosolic side of membrane

18
Q

Adaptor proteins form a second layer between what?

A

cage and membrane

19
Q

Assembly of the clathrin coat protein molecules introduces a ___ in the membrane; and leads to the formation of ___ __

A

curvature; coated buds

20
Q

In regards to assembly of the clathrin coat, daptor proteins bind what?

A

clathrin and membrane-bound cargo receptors

21
Q

These play a major role in coat assembly, vesicle formation, and protein trafficking?

A

Phosphoinositides (PI)

22
Q

True or False?

Interconversions between various PI is highly compartmentalized. It is organelle and domain specific.

A

true

23
Q

Different proteins bind with high specificity to the ___ of particular PI

A

headgroups

24
Q

In regards to removal of the clathrin coat, what protein assembles a ring around the clathrin bud?

A

dynamin

25
Q

Dynamin contains two domains. what are they?

A

PIP2 binding domain: tethers protein to membrane; GTPase domain: regulates rate of vesicle pinching

26
Q

In regards to removal of the clathrin coat, ___ brings the 2 cytosolic sides of the membrane together and allows them to fuse

A

pinching

27
Q

Dynamin recruits other proteins that do what to the lipid bilayer?

A

distort it

28
Q

In regards to removal of clathrin coat, Hsp 70 chaperone protein uses ATP to do what?

A

peel off coat

29
Q

In regards to vesicle targeting, specificity is achieved by what ?

A

surface markers on vesicles and complementary receptors on target membrane

30
Q

In regards to vesicle targeting, this protein directs vesicle to specific spots on target membrane

A

Rab

31
Q

In regards to vesicle targeting, this protein mediates fusion of vesicle with membrane

A

SNARE

32
Q

The v-SNARE and T-SNARE interaction allows them to wrap around each other and form a bundle. The resulting SNARE complex does what?

A

locks the vesicle and target membrane together

33
Q

What is the sequence of events in docking and fusion of vesicle?

A

Rab-GTP tethers with Rab-effector bringing vesicle in closer to target membrane. As docking occurs, V-SNARE and T-SNARE complex forms allowing the vesicle to fuse with the target membrane. Rab-GDP with bound GDI dissociates

34
Q

After the vesicle has fused, what allows the SNARE complex to dissociate?

A

NSF and accessory protein separation by formation of ADP + Pi

35
Q

In regards to transport from ER, what is quality control?

A

incompletely folded/misfolded proteins are retained in ER by binding to chaperone proteins such as BiP or calnexin and are then degraded

Molecular Biology (17 decks)

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