Very Much Flashcards
Inhibitors of DNA polymerase
nitrate, crystals, hemoglobin, and beta-human chorionic gonadotropin
critical for monitoring reagents for carry-over contamina- tion.
Reagent blank or contamination controls
aimed at a target that is always present can be incorporated into an amplification assay.
amplification control
are amplification controls that mon- itor particular steps of an amplification method
Internal controls
FDA-Approved/Cleared Test Methods
Methicillin Resistant Staphylococcus aureus
Real-time PCR
Mycoplasma pneumoniae
16S rDNA
P1 adhesion gene
Chlamydophila pneumoniae
Cloned Pst I fragment
16S rRNA
MOMP
Legionella
5S rRNA mip gene
Bordetella pertussis
IS 481
Streptococcus pneumoniae
lytA (autolysin)
pbp2b
Mycobacterium tuberculosis
16S rRNA
test takes 6.5–8 hours to complete
AMPLICOR MTB
Treponema pallidum
polA
Mycoplasma genitalium
rDNA gene
Ureaplasma urealyticum
Urease gene
Haemophilus ducreyi
groEL gene
p27
Neisseria gonorrhoeae
opa gene
Chlamydia trachomatis
MOMP
Inhibits protein synthesis
Macrolides (erythromycin)
Staphylococcus aureus
Oxacillin - mecA
Streptococcus pneumoniae
Penicillin - pbp1a and pbp1b
Enterococcus
Vancomycin - vanA
Salmonella
Quinolones - gyrA
Mycobacterium
tuberculosis
Rifampin - rpoB
This method has been used for typing numerous species, including strains of Pseudomonas aeruginosa, Mycobacterium avium, Escherichia coli, N. gonorrhoeae, VRE, and MRSA.
Pulsed Field Gel Electrophoresis
Criteria for PFGE Pattern Interpretation
Closely related
‘
1 2–3. Test isolate is closely related to the outbreak strain
This method is performed by cutting DNA with restriction enzymes, resolving the resulting frag- ments by gel electrophoresis, and then transferring the separated fragments to a membrane for probing with a specific probe.
Restriction Fragment Length Polymorphism Analysis
Performance Comparison of Representative Molecular Epidemiology Methods
Plasmid analysis
Good -> Good -> Good -> High -> Good
Performance Comparison of Representative Molecular Epidemiology Methods
PFGE
High -> High -> High -> Moderate -> Good-Moderate
Performance Comparison of Representative Molecular Epidemiology Methods
Chromosomal RFLP
High -> Good -> Good -> High -> Moderate-poor
Performance Comparison of Representative Molecular Epidemiology Method
PCR-RF LP
Good -> Moderate -> Good -> High -> High
Performance Comparison of Representative Molecular Epidemiology Method
Sequencing
High -> High -> High -> Moderate -> Good-High
