veld notes

Question 1

to ensure secured sample transportation, covered containers in ____

Answer 1

• covered
  containers in 10% buffered formalin

Question 2

2 kinds of preservation techniques most commonly used

Answer 2

refrigeration and chemical preservation

Question 3

process by which constituents of cells
are treated in a chemical so that they will withstand subsequent treatment with various reagents with minimum distortion or decomposition and keep the tissue in as life-like manner as possible

Answer 3

fixationn

Question 4

most commonnn tyype of fixationn

Answer 4

immersion

Question 5

most commonn fixative

Answer 5

10% fformalinn

Question 6

optimun size for secctionns innn tissuue procesisng

Answer 6

2x2x0.3cm

Question 7

optimum thicknnness

Answer 7

3-4mm

Question 8

4 stages innn oorder

Answer 8

dehydration, clearinngg, impregnnationn.embeddinng

Question 9

pecision instrument that cuts sections from the paraffin blocks, thin enough for examination under microscope

Answer 9

microtome

Question 10

xylenne is for?

Answer 10

xylene for deparaffinization —> removal of wax

Question 11

enables removal of excess stain

Answer 11

differenntiationn

Question 12

inn rehydrationn, _____ is used for blue ccolor

Answer 12

lithium ccarbonnate

Question 13

innncomplete depraffinnizatioon will result inn what

Answer 13

patches

Question 14

what media is used for mountinng nni sir owel

Answer 14

albuminnn + glycerin

Question 15

what is the most ccommonnn stain annd its other term

Answer 15

hematoxylin and eosin stain (H&E); aka “routine staining”.

Question 16

stainn to identigy diff types ngn tumors

Answer 16

Van Gieson stain

Question 17

a metachromatic dye (acidophilic)

Answer 17

Toluidine blue

Question 18

a mixture of three
dyes

Answer 18

Masson’s trichrome stain

Question 19

stain used to identify mucoid substances and presence of fungi.

Answer 19

PAS (Periodic acid Schiff)

Question 20

this process is usually used on tumor cases

Answer 20

Cryoembedding

Question 21

While awaiting the stain line, prepared slides should be immediately immersed in ___

Answer 21

in formal alcohol, 95% alcohol

Question 22

in cryoembedding, the ideal thhicknnness for secitoniung is

Answer 22

4 - 7 microns and 20 - 40 microns for trimming.

Question 23

involves the pretreatment of tissue to retrieve antigens masked by fixation and make them more accessible to antibody binding

Answer 23

Antigen retrieval (AR)

Question 24

Standard before PCR

Answer 24

ELISA

Question 25

in elisa, darker color meanns more Ab

Answer 25

t

Question 26

t or f: test is positive if tthere is HEX fluorescennnce signnal alonne

Answer 26

f. need din nng FAM or quasar

Question 27

adv nng serology

Answer 27

logner detectionn time

Question 28

use of DIVAC

Answer 28

diff bet innnfeccted at vacccinnated

Question 29

normal fecal score?

Answer 29

2-2.5

Question 30

LAMP ay used for

Answer 30

detection of Anaplasma marginale (blood parasite of cattle)

Question 31

samples should be held onn the bottom part in LAMP: t or f

Answer 31

f. should NNOT kasi sennsitive sa temp

Question 32

how many blocks meronn sa pcr machinne

Answer 32

3

Question 33

inn gel electrophoresis, whhat buffer is usedd

Answer 33

TAE (Tris-acetate-EDTA) buffer

Question 34

what is thhe basis for the bannd size

Answer 34

DNA ladder

Question 35

we are after thick, thin, or multiple bannds: t or f

Answer 35

f. bawal multiple

Question 36

Only sp. That detects antibodies

Answer 36

Dirofilaria immitis

Question 37

what babesia ang present sa ph

Answer 37

B. vogeli;B. gibsoni

Question 38

reads thhe result of elisa

Answer 38

microplate reader

Question 39

what line sa well annng iinntayinnn na bbumaba anng color…

Answer 39

3rd linne from thhe bbottom

Question 40

most pathogeic sa babesia

Answer 40

Rossi - most pathogenic,

Question 41

adv ngn nested pcr

Answer 41

more speccific at sennsitive

Question 42

what shhape ngn struccture sa LAMP

Answer 42

ddumbell