veld notes Flashcards
to ensure secured sample transportation, covered containers in ____
- covered
containers in 10% buffered formalin
2 kinds of preservation techniques most commonly used
refrigeration and chemical preservation
process by which constituents of cells
are treated in a chemical so that they will withstand subsequent treatment with various reagents with minimum distortion or decomposition and keep the tissue in as life-like manner as possible
fixationn
most commonnn tyype of fixationn
immersion
most commonn fixative
10% fformalinn
optimun size for secctionns innn tissuue procesisng
2x2x0.3cm
optimum thicknnness
3-4mm
4 stages innn oorder
dehydration, clearinngg, impregnnationn.embeddinng
pecision instrument that cuts sections from the paraffin blocks, thin enough for examination under microscope
microtome
xylenne is for?
xylene for deparaffinization —> removal of wax
enables removal of excess stain
differenntiationn
inn rehydrationn, _____ is used for blue ccolor
lithium ccarbonnate
innncomplete depraffinnizatioon will result inn what
patches
what media is used for mountinng nni sir owel
albuminnn + glycerin
what is the most ccommonnn stain annd its other term
hematoxylin and eosin stain (H&E); aka “routine staining”.
stainn to identigy diff types ngn tumors
Van Gieson stain
a metachromatic dye (acidophilic)
Toluidine blue
a mixture of three
dyes
Masson’s trichrome stain
stain used to identify mucoid substances and presence of fungi.
PAS (Periodic acid Schiff)
this process is usually used on tumor cases
Cryoembedding
While awaiting the stain line, prepared slides should be immediately immersed in ___
in formal alcohol, 95% alcohol
in cryoembedding, the ideal thhicknnness for secitoniung is
4 - 7 microns and 20 - 40 microns for trimming.
involves the pretreatment of tissue to retrieve antigens masked by fixation and make them more accessible to antibody binding
Antigen retrieval (AR)
Standard before PCR
ELISA
in elisa, darker color meanns more Ab
t
t or f: test is positive if tthere is HEX fluorescennnce signnal alonne
f. need din nng FAM or quasar
adv nng serology
logner detectionn time
use of DIVAC
diff bet innnfeccted at vacccinnated
normal fecal score?
2-2.5
LAMP ay used for
detection of Anaplasma marginale (blood parasite of cattle)
samples should be held onn the bottom part in LAMP: t or f
f. should NNOT kasi sennsitive sa temp
how many blocks meronn sa pcr machinne
3
inn gel electrophoresis, whhat buffer is usedd
TAE (Tris-acetate-EDTA) buffer
what is thhe basis for the bannd size
DNA ladder
we are after thick, thin, or multiple bannds: t or f
f. bawal multiple
Only sp. That detects antibodies
Dirofilaria immitis
what babesia ang present sa ph
B. vogeli;B. gibsoni
reads thhe result of elisa
microplate reader
what line sa well annng iinntayinnn na bbumaba anng color…
3rd linne from thhe bbottom
most pathogeic sa babesia
Rossi - most pathogenic,
adv ngn nested pcr
more speccific at sennsitive
what shhape ngn struccture sa LAMP
ddumbell
