RSJ Flashcards
exchange system; large in
young embryos)
amnion
(respiration and pressure
adjustments)
air sac
(barrier and exhange of
gas and liquid molecules)
shell membrane
route of entry for viral inoculation in the exercise
chorioallantoic sac
Virus replicates in cells lining the
____ and virus released
into ___
chorioallantoic sac
allantoic fluid
how many ml is inoculated?
0.2 ml
in tissue culture exercise, wats the recommended size of tissue embryo
0.5-1 mm
how much PBS?
how much 0.25 % trypsin?
10 ml
0.5 ml
use of trypsin
enzymatic disaggregation
size of SPL cell strainer
70um
example of natural passive immunity
MAb
example of artificial passive
antiglobulin infusion
example natural active immunity
getting infected by wild-type virus
example of artificial active
vaccination
uses of ELISA
confirmation of disease
vaccine response
MAb of young animals
most common format of ELISA
indirect format
recommended freeze-thaw cycles should not be more than ___cycles
3-5
major steps in NA extraction
lysis
binding/capture
washing
elution
what are the primer-probe combinations
- PEDV RNA probe = FAM fluorescence
- TGEV RNA = Quasar® 670/Cy®5 fluorescence
- Heterologous RNA control = HEX fluorescence
- An internal control
- Checks for false-negative results
Heterologous RNA control
contains target viral RNA
* checks for correct set-up of the reaction mix
pos control
Enables assessment of contamination in the
reaction
neg control
checks integrity of sample
* checks for possible PCR inhibition
internal control
maching for PCR
thermocycler
positive control CT
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