RSJ

Question 1

exchange system; large in
young embryos)

Answer 1

amnion

Question 2

(respiration and pressure
adjustments)

Answer 2

air sac

Question 3

(barrier and exhange of
gas and liquid molecules)

Answer 3

shell membrane

Question 4

route of entry for viral inoculation in the exercise

Answer 4

chorioallantoic sac

Question 5

Virus replicates in cells lining the
____ and virus released
into ___

Answer 5

chorioallantoic sac
allantoic fluid

Question 6

how many ml is inoculated?

Answer 6

0.2 ml

Question 7

in tissue culture exercise, wats the recommended size of tissue embryo

Answer 7

0.5-1 mm

Question 8

how much PBS?
how much 0.25 % trypsin?

Answer 8

10 ml
0.5 ml

Question 9

use of trypsin

Answer 9

enzymatic disaggregation

Question 10

size of SPL cell strainer

Answer 10

70um

Question 11

example of natural passive immunity

Answer 11

MAb

Question 12

example of artificial passive

Answer 12

antiglobulin infusion

Question 13

example natural active immunity

Answer 13

getting infected by wild-type virus

Question 14

example of artificial active

Answer 14

vaccination

Question 15

uses of ELISA

Answer 15

confirmation of disease
vaccine response
MAb of young animals

Question 16

most common format of ELISA

Answer 16

indirect format

Question 17

recommended freeze-thaw cycles should not be more than ___cycles

Answer 17

3-5

Question 18

major steps in NA extraction

Answer 18

lysis
binding/capture
washing
elution

Question 19

what are the primer-probe combinations

Answer 19

1. PEDV RNA probe = FAM fluorescence
2. TGEV RNA = Quasar® 670/Cy®5 fluorescence
3. Heterologous RNA control = HEX fluorescence

Question 20

• An internal control
• Checks for false-negative results

Answer 20

Heterologous RNA control

Question 21

contains target viral RNA
* checks for correct set-up of the reaction mix

Answer 21

pos control

Question 22

Enables assessment of contamination in the
reaction

Answer 22

neg control

Question 23

checks integrity of sample
* checks for possible PCR inhibition

Answer 23

internal control

Question 24

maching for PCR

Answer 24

thermocycler

Question 25

positive control CT

Answer 25

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