Variant 4-monolayer

Question 1

– Determination of the Size of
Amphipathic Molecules by Monolayer
1. Explain the aim of the study for this practice. (5 points)

Answer 1

To determine the length average area per molecule and the diameter of (oleic) acid
molecule using monolayer

Question 2

Show the graph for the dependence of the pressure on the change of the area of the
monolayer. (8 points)

Answer 2

look in my book just a slop down

Question 3

Describe shortly experimental work for this practice. (7 points)

Answer 3

• 2 paper tapes covered with paraffinon glass container with watwer
• talc powder in btw the tapes
• Drop some oleic acid onto the water it to create monolayer. Distance below tapes are measured
• reduce area of monolayer by mving the talc up further
• once signs of elastic deformation shows- solid monolayer
• Mesure surafce area, length an isamter of molecule

Question 4

Give some examples for the application of the monolayer technique. (5 points)

Answer 4

Investigate properties of alveolar Surfectant

Contacts between molecules

Question 5

Write the equation for osmotic pressure with the aggregation of erythrocytes. (7 points)

Answer 5

Π=CRT (100% dissociated ideal solution)
* Π=iCRT (<100% dissociated ideal solution) i=1 non electrolyte i>1 electrolyte

Question 6

Define the meaning of iontophoresis. (7 points)

Answer 6

Application of electrophoresis for drug delivery (non-invasive method)
Transcutaneous/ trans mucous

Question 7

Indicate the main factors, associated with the aggregation of erythrocytes. (7 points)

Answer 7

Extracellular factors:
Flow, plasma proteins, ph, ionic strenght, osmotic pressure

Cellular factors:
glycoalx, rbc deformabiliy and shape, electrokinetic potential, surface change density