Varaint 4A- erythrocyte deformability Flashcards
Questions concerning influence of erythrocyte deformability on the relative viscosity of erythrocyte suspensions
The aim of the study (5)
To investigate the influence of cell deformability of RBCs on the relative viscosity of erythrocyte suspension
Define the term erythrocyte deformability, which cellular factors determine this cell property
(7)
Mechanical propert of rbcs to chnage their shape PASSIVELY as a result of applied stress
- geometric parameters
- viscosity of cytoplasm
- viscoeleasticity properties of RBC
- temperature of medium
- Osmotic pressure
*age
Describe shortly the experimental work and measurements performed in this practice (8)
the erythrocyte deformability
- RBC suspension HCT 60% 4ml
- then 1 testube will be control with 1ml of pbs added
- 2 testubes will have 1ml of GDA also added, 0.1% and 0.2%
- testubes put in oswald viscomter to detrmine realtive viscosity
- Calcute: relative viscosity= RBC/ RBC in pbs
How is the relative viscosity of the suspension of glutaraldehyde hardened erythrocyte
changed as compared to suspension of native RBC? (5)
As concentration increases, the relative viscpsity also increases. This is because there is decreased deforbability so RBC cannot chnage shape as easily
Represent schematically the double electric layer of flat surface with negative charge (7)o
stern
Below is given the definition expression for the coefficient of iontophoretic permeability of
the skin(k). Explain the meaning of the parameters involved in the formula (7)
k=m/ Ixtxs
mass of solute delivered
electric current
time
area the drufg was delivered
k coefficient of iontophoretic permebaility
Write the stokes-Einstein equation for the diffusion coefficient (D). Explain the parameters
involved (7)
D= KBT/6piviscr
m2/s
Plot the dependence of the relative volume of erythrocyte (V) on the osmotic
pressure (1/pi) (5)
Determine from the graph above the osmotically inert volume V, of the RBC (5)
Linear graph ]
extend line to y axis then circle in Vn
