Variant 3-antioxidant and peroxidant activity

Question 1

Questions concerning investigation on antioxidant and prooxidant activity of pharmaceutical and
biological substances
1. Explain the aim of the study (5)

Answer 1

To investigate the influence of the drug chlorazine and blood plasma on the free
radical oxidation of egg lipoproteins in suspension

Question 2

Write the stages for lipid peroxidation with the chemical reactions (8)

Answer 2

initiation
propogation
termination

perozidation by addition of oxygen radical or getting rid of hydrogen

Question 3

List the main types of reactive oxygen species (ROS) and the main enzymes, responsible for
their cleaning in the cells (7)

Answer 3

Triplet (ground state) oxygen 3O2
* Singlet oxygen 1O2
* superoxide radical O2
-
(superoxide dismutase SOD)
* hydroxyl radical .OH (glutathion peroxidase GSH-P)
* hydrogen peroxide H2O2 (Catalase Cat & Glutathion peroxidase GSH-P)
* hydroperoxyl radical .O2H,
* peroxyl radical ROO.
* Hydroperoxide ROOH (Glutathion peroxidase GSH-P)
*OZONE 03

Question 4

Which method we use for estimation of the antioxidant activity and which parameter we
have to measure in the given practice?

Answer 4

Induction of free radical oxidation of egg yolk lipoproteins in Chlorazine

then look at TBA reaction

then plot absorance

Question 5

Ficks first law

Answer 5

J=-D dc/dx

Question 6

Draw a micelle

Answer 6

phospholipids in a sphere

Question 7

Plot the dependence of the specific viscosity on the haematocrit,

Answer 7

linear graph
do change in y over chnage in x for slope