Vaginal Cytology Flashcards
Cells are almost exclusively clusters of round, well-formed nucleated epithelial cells. The nucleus will stain darker than the cytoplasm
Proestrus
Cells are predominantly cornified squamous epithelial cells, present in densely packed clusters.
Estrus
These cells lack a nucleus and have an angular appearance.
Estrus
Small darkly stained leukocytes predominate
Metestrus
. Cornified squamous epithelial cells may be observed, often in fragments.
Metestrus
The leukocytes are generally neutrophils with sausage-link nuclei which stain very dark purple.
Metestrus
The predominant cells are leukocytes.
Diestrus
Also present will be nucleated epithelial cells and rarely cornified squamous epithelial cells.
Diestrus
Where should be the sample be collected from
cranial vagina
This cell look like small O shaped oat cereal pieces
Parabasal Cells
this looks like a fried eggs
intermediate cells
cells which looks like corn flakes; neutrophils and red blood cless
superficial cells
4 phases of estrous cycle
anestrus (not in heat)
proestrus (early estrogen influence)
estrus (receptive, fertile)
diestrus (luteal phase)
parabasal cells predominate on this phase
anestrus
a gradual shift from parabasal and intermediate cells and finally superficial cells occur
proestrus
superficial cells predominate and their nuclei become pyknotic or absent/anuclear
estrus
marked by precipitous decline in the number of superficial cells and reappearance of intermediate and parabasal cells within 1 to 2 days
diestrus
neutrophils are commonly observed on this phase and large numbers of bacteria are also often present
diestrus ?(luteal phase)
composed of parabasal cells small and large intermediate cells; a few superficial cells
early proestrus
predominantly superficial cells and medium to large intermediate cells. The edges are straight and the cells are flattened
Late Proestrus
The cells are cornified
Estrus
onset of this phase is characterized by the sudden appearance of parabasal cells; a change from predominantly noncornified
diestrus
what are the 4 different types of cells
parabasal cells
small intermediate cells
large intermediate cells
superficial cells
metestrus cells
this type of cell slightly larger than parabasal cells, borders irregular
small intermediate cells
large cells with irregular borders
large intermediate cells
largest cells seen; borders irregular, no nucleus present
superficial cells
small, round, slightly oval, large vesiculated nucleus and small cytoplasm.
Parabasal cells
parabasal cells with infiltration of a varying number of nucleated cells
metestrus cells
slightly larger than parabasal cells to twice that size
Intermediate cells
Smooth, oval to rounded irregular borders, nucleus smaller than in parabasal cells. More cytoplasm than parabasal cells.
Intermediate cells
Are dead cells, are the largest in vaginal cytology with a sharp, flat, angular cytoplasmic borders and a small pyknotic, fading nuclei or without nuclei.
Superficial cells
irregular vaginal cells, no nucleus, smaller than superficial. These are the cells that have also been called “fully cornified.”
Anuclear squames
Large intermediate vaginal cells that appear to have one or more neutrophils in their cytoplasm. Are typical of bitch diestrus.
Metestrus cells
parabasal and intermediate cells with obvious cytoplasmic vacuoles. May be associated with diestrus and anestrus.
Foam cells
Inflammatory cells that can be normal or abnormal in vaginal cytology in function of the estrus period
Neutrophils
Blood can be normal in the bitch but always is abnormal in the queen
RBC
