vacuole inheritance Flashcards
vac8 binds vacuole and recruits what?
vac17 which recruits myo2
what phosphrorylates vac17?
cdc28
what mediates temporal regulation of vacuole transport`?
phosphorylation of vac17 and myo2
vac17 is spatially regulated by being broken down where?
in bud
what does dma1 recognise?
phosphorylated T240 in PEST sequence of vac17
what does dma1 do to vac17?
ubiquitinates it to release it from myo2 and can be degraded by proteasome
what must also phosphorylate vac17 S222?
cla4
what recruits cla4?
cdc42
what is s222 phosphorylation required for?
Dma1 dependent vac17 phosphorylation
what inhibits kin4 in bud
cla4 which recruits lte1 that inhibits cla4
what inactivates the dma1 dependent breakdown pathway?
kin4 and frk1 in mother cell
why are kin4 and frk1 required for vacuole transport
they prevent breakdown of vac17 in mother
what is FM4-64
a dye that is endocytosed and ends up in vacuoles
