Using Restriction Endonucleases

Question 1

What are restriction endonucleases, and what is their role in bacteria?

Answer 1

Restriction endonucleases are enzymes produced by bacteria to defend against viral infections by cuttin up viral DNA

Question 2

What isa. recognition sequence in the context of restriction endonucleases?

Answer 2

It is a specific sequence of bases where a restriction endonuclease cuts the DNA

Question 3

What are the two types of cute made by restriction endonucleases?

Answer 3

1. Blunt ends: Straight cuts between two opposite base pairs
2. Sticky ends: Staggered cuts leaving unpaired bases on each DNA strand

Question 4

What are blunt ends, and how are they formed?

Answer 4

Blunt ends are straight edges of DNA formed when a restriction endonuclease cuts directly between two opposite base pairs

Question 5

Why are sticky ends described as palindromic?

Answer 5

The sequences of unpaired bases at each end read as opposites when read from left to right, making them a palindrome

Question 5

What are sticky ends, and how are they formed?

Answer 5

Sticky ends are uneven DNA cuts with exposed unpaired bases, formed when a restriction endonuclease cuts in a staggered fashion

Question 6

WHat does the term “six bp palindromic sequence” mean?

Answer 6

It refers to a palindromic recognition sequence that is six base pairs long

Question 7

Why are sticky ends important in genetic engineering?

Answer 7

Sticky ends allow DNA fragments to be joined easily by complementary base pairing, facilitating the insertion of genes into vectors or other DNA molecules