Use Of Microscope

Question 1

List the parts of the light microscope from top to bottom

Answer 1

1Eyepiece or ocular lens

#2body tube
#3 arm
#4revolving nose piece
#5high objective lens
#6 low objective lens
#7stage
#8stage clips
#9 diaphragm and condenser
#10 mirror or electric light source
#11 coarse adjustment knob
#12 fine adjustment knob
#13base

Question 2

What is a light microscope

Answer 2

The microscope that uses light to magnify an object

Question 3

What is the function of an eyepiece

Answer 3

It is the lens that you place your eye against to magnify the object being examined normally 10x

Question 4

What is the function of a tube

Answer 4

Keeps the lenses of the eyepiece and objectives at the correct working distance from one another

Question 5

What is the function of a coarse adjustment knob

Answer 5

It moves the stage up and down to focus on the object being examined

Question 6

Function of the Fine adjustment knob

Answer 6

It is used for the final focus to get a clear image of the object

Question 7

Function of the arm

Answer 7

Connects the base and stage with the tube and supports the adjustment knobs

Question 8

Function of the revolving nosepiece

Answer 8

Holds the objectives with low and high power lenses closely to the rotating slide

Question 9

Functions of the objectives

Answer 9

Lenses of objectives in magnifying the image :
The short lens gives a lower magnification.(4 x)
The long lenses give a higher magnification (10 x and 40 x)

Question 10

Function of the stage clips

Answer 10

Keeps the microscope slide firmly in position to examine the object.

Question 11

Function of the stage

Answer 11

Supports the microscope slide over the opening that allows light through from the mirror or light source

Question 12

Function of the diaphragm and condenser

Answer 12

Controls the amount of light that moves to abs through the object;focuses light beams from the mirror or electrical source onto the object

Question 13

Mirror or electrical source

Answer 13

Reflects the light upwards through the condenser to the opening in the stage

Question 14

Function of the base

Answer 14

It is sturdy abd supports the weight of the microscope

Question 15

Define magnification

Answer 15

If the magnifying power of the eyepiece is 10x,thr objective is 40x then the total magnification of the object is 10 x 40 or 400x.

Question 16

Formula of magnification

Answer 16

Magnification =Ocular magnification X objective magnification
E . G. Magnification =10 x 40 =400
The images is therefore 400x larger than the specimen

Question 17

Microscope slide

Answer 17

Consists of a small object glass, the object(specimen) and a cover glass

Question 18

Micrograph

Answer 18

Is a photo taken by a microscope

Question 19

Magnification

Answer 19

Making the picture appear larger

Question 20

Resolution

Answer 20

Ability to distinguish between two objects because they can look like one object. The microscope needs 0.2 microns or more apart in order 5o see that they are different objects

Question 21

Explain the 2 types of light in a light microscope

Answer 21

Bright field light microscope tend to be what people are familiar with, typically producing a darker image on a light background
With the dark field light, you have a piece that blocks the source light called the light stop under the condensing lens, the blocked light is to see the light reflected or refracted from structures within the specimen that you are looking at.

Question 22

Different types of light microscope

Answer 22

Phase contrast
Differential interference contrast
Fluorescenes
Confocal
Two photon

Question 23

If we want to see a virus

Answer 23

We use an electronic telescope

Question 24

The microscope was invented in about 1590 by a….and what does it show

Answer 24

Dutch spectacles maker called
Zacharias Janssen. Since that time the microscope has helped scientists to discover
what plants and animals are made up of and how cells work.

Question 25

A light microscope, like
the ones most often used, can magnify things up to

Answer 25

about x1500

Question 26

Electron
microscopes can magnify things up to about

Answer 26

x1 500 000.

Question 27

ROBERT HOOK

Answer 27

Saw dead cells in cork

Question 28

Lwoff

Answer 28

1962
Cell Theory- unitity concept

Question 29

mounting medium

Answer 29

specimen placed in a drop of liquid/ any other liquid

Question 30

1000um

Answer 30

1mm