Use Of Microscope Flashcards
List the parts of the light microscope from top to bottom
1Eyepiece or ocular lens
#2body tube
#3 arm
#4revolving nose piece
#5high objective lens
#6 low objective lens
#7stage
#8stage clips
#9 diaphragm and condenser
#10 mirror or electric light source
#11 coarse adjustment knob
#12 fine adjustment knob
#13base
What is a light microscope
The microscope that uses light to magnify an object
What is the function of an eyepiece
It is the lens that you place your eye against to magnify the object being examined normally 10x
What is the function of a tube
Keeps the lenses of the eyepiece and objectives at the correct working distance from one another
What is the function of a coarse adjustment knob
It moves the stage up and down to focus on the object being examined
Function of the Fine adjustment knob
It is used for the final focus to get a clear image of the object
Function of the arm
Connects the base and stage with the tube and supports the adjustment knobs
Function of the revolving nosepiece
Holds the objectives with low and high power lenses closely to the rotating slide
Functions of the objectives
Lenses of objectives in magnifying the image :
The short lens gives a lower magnification.(4 x)
The long lenses give a higher magnification (10 x and 40 x)
Function of the stage clips
Keeps the microscope slide firmly in position to examine the object.
Function of the stage
Supports the microscope slide over the opening that allows light through from the mirror or light source
Function of the diaphragm and condenser
Controls the amount of light that moves to abs through the object;focuses light beams from the mirror or electrical source onto the object
Mirror or electrical source
Reflects the light upwards through the condenser to the opening in the stage
Function of the base
It is sturdy abd supports the weight of the microscope
Define magnification
If the magnifying power of the eyepiece is 10x,thr objective is 40x then the total magnification of the object is 10 x 40 or 400x.
Formula of magnification
Magnification =Ocular magnification X objective magnification
E . G. Magnification =10 x 40 =400
The images is therefore 400x larger than the specimen
Microscope slide
Consists of a small object glass, the object(specimen) and a cover glass
Micrograph
Is a photo taken by a microscope
Magnification
Making the picture appear larger
Resolution
Ability to distinguish between two objects because they can look like one object. The microscope needs 0.2 microns or more apart in order 5o see that they are different objects
Explain the 2 types of light in a light microscope
Bright field light microscope tend to be what people are familiar with, typically producing a darker image on a light background
With the dark field light, you have a piece that blocks the source light called the light stop under the condensing lens, the blocked light is to see the light reflected or refracted from structures within the specimen that you are looking at.
Different types of light microscope
Phase contrast
Differential interference contrast
Fluorescenes
Confocal
Two photon
If we want to see a virus
We use an electronic telescope
The microscope was invented in about 1590 by a….and what does it show
Dutch spectacles maker called
Zacharias Janssen. Since that time the microscope has helped scientists to discover
what plants and animals are made up of and how cells work.
A light microscope, like
the ones most often used, can magnify things up to
about x1500
Electron
microscopes can magnify things up to about
x1 500 000.
ROBERT HOOK
Saw dead cells in cork
Lwoff
1962
Cell Theory- unitity concept
mounting medium
specimen placed in a drop of liquid/ any other liquid
1000um
1mm
