Urogenital Flashcards
- What is tunica dartos? Action?
a. Smooth muscle that separates cavities and helps draw testes to abdomen / body
- What is between outer/inner tunics?
a. “Vaginal cavity” – continuous with peritoneal cavity at external inguinal ring
- What type of cells do you see on cytology during estrus?
a. Keratinized cornified epithelium, superficial cells
b. Some bacterial flora
c. Should NOT see neutrophils (except in diestrus)
- Give origin / insertion of each muscle and general location on penis
a. Retractor penis
b. Ischiocavernosus
c. Bulbospongiosus
d. Ischiourethralis
a. Retractor penis – smooth muscle caudal half with external anal sphincter – ventral surface insert on penis at level of preputial fornix
b. Ischiocavernosus
i. O: ischial tuberosity
ii. I: proximal corpus cavernosum
c. Bulbospongiosus
i. O: from tunica albuginea and EAS covers bulb of penis; fuse with retractor peenis at 1/3 of body
d. Ischiourethralis
i. O: dorsal ischial tuberosity
ii. I: fibrous ring at urethral bulb
- Where do proper ligament of testis & ligament of tail of epididymis attach?
a. Epididymal tail to testis
b. Testis / epididymis to vaginal tunic & spermatic fascia
- Describe lymph & nervous systems of prostate
a. Lymph: medial iliac + hypogastric chain of nodes
b. Nerves:
i. Fluid excretion / secretion: cholinergic post-ganglionic hypogastric (S)
ii. Smooth mm contraction: adrenergic post-gang hypogastric (S)
iii. +/- parasymp pelvic n to increase rate secretion
iv. Stromal tissue: nonadrenergic to control smooth mm tone, several NTS
- What is diameter of feline ureter? Dog ureter?
a. Feline: 0.4 mm
b. Dog: “0.07 x length of L2 body” (studies show 2-2.5 mm on CT)
- Describe vascular & nerve supply to ureter
a. Vascular: Ureteral a from caudal aspect of renal a ureteric branch of caudal vesicular a
b. Nerve: ANS – PS/S from pelvic plexus; S – celiac plexus
- What are macula densa?
a. Cells outside of glomerulus that maintain autoregulation of blood flow
- What size particles filter in glomerulus?
a. <60,000 Daltons
- What charged molecules can’t filter in glomerulus?
a. Negative (like albumin)
- What is normal urine production?
a. 20-45 ml/kg/day
- What % of cardiac output is through kidney at all times?
a. 25%
- During embryonic development, what is cranial vagina formed from?
a. Paired paramesonephric (Mullerian) ducts
- What is the genital tubercle?
a. Analogous to penis or vaginal clitoris (lots of nerve endings)
- What is the uterine ostium?
a. The opening of uterine tube to the uterine body (acts like sphincter)
- What is different about the cat with respect to ovarian tube?
a. Tube tortuous and can be seen within mesosalpinx
- When does mucosal healing occur in bladder?
a. 5 days
- How long until 100% bladder tissue strength?
a. 14-21 days
- What are the accessory sex glands of dogs vs cats?
a. Dog: prostate
b. Cat: bulbourethral gland (thick mucous) + prostate (alkaline fluid for survival of sperm)
- Describe the blood supply, LN, nerves to vagina/vestibule, vulva
a. Arteries:
i. Vagina, urethra, vestibule – vaginal a from internal pudendal
ii. Vulva – external pudendal
b. Venous: same as arterial
c. LN:
i. Vagina/vestibule – internal iliac LN
ii. Vulva – superficial iliac LN
d. Nerves:
i. PS – pelvic
ii. S – hypogastric
iii. Sensory – pudendal
- Describe innervation and micturition reflex
a. Urine fill / retention -> sympathetic via hypogastric
i. + alpha at IUS to contract
ii. + beta 3 on detrusor – relaxes detrusor
b. Urination – full bladder – stretch on m3 receptor – afferent pelvic – pontine contract
i. Parasym via pelvic n – binds m3 to contract detrusor – pees
ii. Inhibits pudendal & hypogastric
1. Pudendal – somatic efferent to m3 on external urethral sphincter
See fig 116.3 bladder
- What is contained in spermatic cord?
a. Ductus deferens (ductus a/v), testicular a, pampiniform plexus, lymph, nerves, cremaster m
- What forms spermatic fascia?
a. Transversalis, superficial / deep abdominal fascia
- What forms cremaster m? Action?
a. IAO, transversus abdominus
b. Raises / lowers testes
- Average size of dog / cat ovaries?
a. Dog: 15 x 7 x 5 mm
b. Cat: 8-9 mm long
- What is the vaginal process?
a. Peritoneal fold that encloses round ligament as passes through inguinal canal
- Vascular, LN, nerve supply to ovaries?
a. Ovarian a from aorta
b. R ovarian V -> Cd VC
c. L ovarian V -> L renal V
d. Lumbar LNs
e. Sympathetic division of ANS
- Describe vasculature, nerve, and lymph supply to testes / epididymis
a. Testes:
i. Testicular a from aorta
ii. Testicular V -> forms pampiniform flexus
1. R testicular V drains into -> Cd VC
2. L -> L renal V + ductus deferens vein
b. Epididymis:
i. Ductus deferens a - branch of prostatic a – arises from branch of internal iliac
c. Nerves: testicular (internal spermatic plexus)
i. Epi (L4-L6 ganglia of sympathetic trunk)
d. Lymph: lumbar LN
- What are main differences between feline & canine reproductive cycles?
a. Feline: seasonally polyestrus (+/- 5th “nonestrus” phase)
i. Vulva not responsive to estrogen
ii. CL requires induction of ovulation via copulation (CL functional 37 d in non-preggo cats)
iii. Bone mineralization seen 25-29 days before birth (week earlier than dogs)
iv. Placental secretion of progesterone independent of ovaries – occurs after day 40
- Where do preputial muscle originate? Insert?
a. O: xiphoid cartilage
b. I: dorsal wall of prepuce
c. Derived from cutaneous trunci
- What maintains normal prostate lot (secretion?)?
a. Androgens
- What breeds already have prostate in abdomen from birth (to adulthood)?
a. Chondrodystrophic
- What are colliculis seminalis?
a. Slits where prostate gland ejaculation ducts enters prostatic urethra
- What point in growth is prostate development most marked?
a. 20-32 week of age
- Where are the nephrons impermeable to urea?
a. Thick look of Henle
b. Distal tubules
c. Cortical collecting ducts
d. Can be partly absorbed in PCT (medullary collecting tubules?)
- What can you give to tx hyperkalemia for urethral obstruction?
a. If ECG changes or K > 8
i. Ca gluconate 0.5-1.5 ml/kg IV over 5-10 min
ii. Dextrose + insulin
iii. IVF diuresis the best start
- What is relative concentration of renal medullary interstitium vs cortical? Hypertonicity created by __?
a. 1200-1400 mOsm/L vs 300 cortex
b. Diffusion of urea into interstitium at CD (gives 50% of osmolarity)
c. Limited ability of water to diffuse into interstitium (only descending loop)
d. Active transport of Na, K, Cl from thick portion of loop of Henle
- What are the 4 phases of estrus cycle & what are the prominent hormone changes here?
a. Proestrus – 9 days – estrogen elevation & follicle maturity
b. Estrus – 9 days – LH surge as estrogen decreases; also progesterone starting to rise; ovulation w/I 2-3 days after LH surge
c. Diestrus – 60 days – where stays in pregnancy if fertilized; increase progesterone levels
d. Anestrus – 4.5 months – low levels of everything; slow rise of estrogen towards pro/estrus?
- Urethra blood supply & innervation?
a. Nerve: PS (pelvic); S (hypogastric) – smooth m
i. Pudendal – somatic – striated
b. BS: branches of internal pudendal
i. Prostate – urethral
ii. Vaginal – urethral
- What are changes seen on ECG with hyperkalemia from blocked urethra?
a. Spiked T to depress R wave
b. Prolonged QRS and PR intervals
c. ST segment depression
d. Smaller/ wider P with long QT interval
e. Atrial standstill
f. Wide QRS
g. Ventricular arrhythmias
- What are 3 stages of parturition and what are guidelines or concerns?
a. 1: Uterine contractions present ~24 hours, nesting behaviour, etc
b. 2: Expel fetus
c. 3: Expel placenta
d. Active strain <= 30 min before birth; time lab between puppies <4 hours
- 3 major types of cells of testes and roles
a. Spermatogenic cell – form the sperm
i. Mitosis –> spermatocytes –> meiosis to spermatids
b. Sertoli cells – “nurse or sustentacular cells”
i. Nourish and support development of spermatozoa
ii. + by FSH (from the anterior pituitary gland), produce inhibin
iii. Pituitary inhibits FSH; Inhibin inhibits FSH (negative FB)
c. Leydig cells – produce testosterone
i. Dependent on negative feedback with LH
- What are factors that help keep scrotum / testes cool?
a. Cremaster + tunica dartos contracts
b. Rich in sweat glands
c. Little SQ fat
d. Few hair follicles
e. Pampiniform plexus cools blood
- What is blood supply, nerves, lymph for scrotum?
a. External pudendal a scrotal a
b. Scrotal V
c. Nerves: superficial perineal n – branch of pudendal (S1-S3)
i. Tunica dartos inn by post ganglionic sympathetic trunk from superficial perineal n
1. NOT PELVIC PLEXUS
d. Lymph: Superficial inguinal LN
- Describe the muscular layer of ureter
a. tunica muscularis = Inner & outer longitudinal, middle circular layers
b. Pitch of muscle fibers = circular proximally
i. Oblique toward mid-length
ii. Longitudinal distally
- What are the 2 mechanisms of developing an erection?
a. Engorgement of cavernous bodies by expansion of arteries / contraction of veins
b. Distal penile vein compressed against ischial arch by contraction of ischiocavernosus and bulbospongiosus muscles
- Describe blood supply and lymph of bladder
a. Caudal vesical a – prostatic / vaginal internal pudendal
b. Cranial vesical a – from umbilical
c. Internal pudendal v
d. Lymph: hypogastric & sublumbar LN
- Describe vascular supply of kidneys
a. Renal a (left +/- 2) “The left kidney is more likely to have multiple renal arteries than the right kidney. Some single renal arteries branch immediately after leaving the aorta, making it appear that the kidney has two renal arteries”
i. Renal a splits into dorsal and ventral ranches at hilus
ii. Then branch into Interlobar – arcuate (corticomedullary junction) – interlobar – afferent – efferent
iii. Small capsular a – from phrenicoabdominal / adrenal
iv. Vasa recta – wrap around nephron
b. Venous
i. Deep & superficial v within renal parenchyma – stellate – interlobar – arcuate – renal v – CdVC
ii. Left renal V also gets blood from L ovarian or L testicular V
- What are the functions of the prostatic secretions? What is it composed of?
a. Promote sperm motility
b. Increase uterine perfusion
c. Modulate neutrophil induced inhibition of spermatozoa attachment to uterine epithelium
d. pH 6.1-6.5, PGE2, Na, K, Cl, + Zn, acid phosphatase + esterase
e. in 3rd fraction of ejaculation
- Gestation length dogs / cats?
a. Dogs: 64 days
b. Cats: 66 days
- When are fetal skeleton detected on rads dogs / cats?
a. Dogs: day 42 (21-24 days before parturition)
b. Cats: 25-29 days before parturition
- When does attachment occur in dogs / cats?
a. Dogs: 21-22 days post LH surge
b. Cats: 15 days after coitus
- What are the changes in bloodwork commonly seen during pregnancy in dogs?
a. Anemia ~<40% at 35 days, <35% at term
b. Mild increases in WBC, cholesterol
c. Decreased protein
d. Increased glucose – insulin resistance
e. Progesterone – decreases 18-30 hours pre-partum
- Describe vascular supply to prostate
a. Internal pudendal – prostatic a -> arteries of ductus deferens - caudal vesical, caudal rectal
b. Anastomoses between Prostatic a – urethral a – cranial & caudal rectal a
c. Cranial / middle / caudal branch – subcapsular a -> supplies glandular tissue
d. Parenchyma, capsular, and urethra vascular zones
e. Venous:
i. Prostatic v & urethral v -> internal iliac v
ii. Prostatic urethra -> prostatic v, v of urethral bulb, ventral prostate veins
- What is the nerve supply to penis? Lymph nodes?
a. Pelvic & sacral plexuses; dorsal n of penis – chief sensory n
b. Superficial inguinal LN
- Describe the vascular supply, lymph centers, and nerve supply to uterus
a. Uterine arteries
i. Branch of vaginal – branch of internal pudendal – branch of internal iliac
b. LN: hypogastric & lumbar
c. N: pelvic plexus S – hypogastric; PS – pelvic
- What are proposed reasons estrogen helps with BPH development?
a. Increased sensitivity of prostate to dihydrotestosterone by inducing nuclear dihydrotestosterone receptors
b. Inhibitory effect on rate of cell death?
- Describe the differences between glandular and complex form of BPH?
a. Glandular:
i. Testosterone metabolized by 5 alpha reductase dihydrotestosterone
ii. Structure and arrangement remain orderly and organized at stage
iii. Increased androgen receptors (maybe less cell death?)
b. Complex:
i. Stromal elements – asymmetric enlargement
ii. Areas of atrophy, cystic alveoli with eosinophilic materials & inflammatory cells
- What are main differences between urethra of male / female dogs / cats?
a. Male dog:
i. No pre-prostatic urethra
ii. Thick circular striated surrounds long smooth in distal 2/3
iii. Long penile component
b. Male cat:
i. Distinct pre-prostatic
ii. Pre and post-prostatic urethra 2mm diameter (prostate)
iii. Bulbourethral glands 1.3 mm
iv. Penile 0.7 mm
v. 3 layers of smooth muscle fibres of preprostatic urethra
vi. Striated (urethralis) muscle short functional length
c. Female dog:
i. Short / wide 0.5 cm
ii. A lot more collagen!
iii. 3 smooth layers
iv. Interdigitate with striated muscle distal 1/3
v. Sphincter of voluntary striated mm at external urethral orifice.
d. Female cat:
i. Smaller lumen than dog
ii. Urethral wall lot more longitudinal smooth & less striated mm
- What are the origins / insertions & what are they intimately associated with?
a. Corpora cavernosa
b. Corpus spongiosum
c. Bulbus glandis
a. Corpora cavernosa
i. Ishial tuberosity dorsal to os penis
ii. Covered by tunica albuginea
b. Corpus spongiosum
i. Within pelvic cavity – surrounds penile urethra
ii. Also shunts blood to bulbus glandis
c. Bulbus glandis
i. Proximal part of os penis
ii. Separated from longa glandis (distal)
iii. Expands way more than long glandis for erection
- What % spays get complications?
a. ~7.9-19% (mostly minor)
- What are options for port locations for OVE/OVH?
a. Transabdominal – all midline
b. Transabdominal – midline + cranial R side
c. Transabdominal – paramedial ports (instruments)
i. All 3 or 2 port
d. Combi – transabdominal + transvaginal
e. Single port transabdominal
- Vessel sealing devices only use on uterus < __ mm
a. 9 mm
- When do you perform laparoscopic artificial insemination (as in bloodwork with cytology)?
a. Progesterone 4-8 ng/mL; when >= 80% superficial cells on vaginal cytology
- What does low serum LH in bitch indicate?
a. Intact status; OR ovarian remnants if spayed <10 days ago
- What does chronic increased plasma estrogen indicate?
a. Follicular cysts
b. Estrogen producing tumors
- What bloodwork changes come with leutinized follicular cysts?
a. Increased progesterone with normal estrogen conc and no signs of proestrus or estrus
- What are advantages of scrotal urethrostomy in male dogs?
a. More superficial, wider, less hemorrhage
b. Less urine scald / UTI / incontinence with others
- What are urethrostomy options for females?
a. Subpubic & prepubic
- What is complication rate for perineal urethrostomy in cats?
a. Newer studies 12-15%
- List surgical techniques for urethrostomy in cats
a. Perineal
b. Transpelvic
c. Subpubic
d. Prepubic
- Which has increased rate of complications?
a. Prepubic
- Prognosis for urethral R&A?
a. Guarded prognosis
- What is epispadias? What could happen with it?
a. Failure of fusion of dorsal penile urethra –> bladder exstrophy
- What breeds at risk for urethral prolapse?
a. Brachycephalic; English Bulldog
- What are tx options for urethral prolapse?
a. Castration
b. Phallopexy
c. Urethral R&A
d. Reduse + purse string
e. Treat BOAS
- Recurrence rates of urethral prolapse post sx? What should you be giving post-op to help decrease?
a. 50-60%; sedation with ace/butorphanol
- List suspected factors affecting causes of USMI
a. Urethral tone & length (shorter)
b. Bladder neck position (pelvic bladder)
c. Body size & breed (overweight, longer breeds)
d. Gonadectomy (affect collagen levels?)
e. Hormonal status (decreased estrogen)
f. Genital conformation (vestibulovaginal stenosis)
- List treatment options for USMI and their success rates
a. Sympathimomimetics or parasympatholytics ~50% alone
i. Estrogen ~50%
ii. Alpha agonist phenylpropanolamine
b. GnRH analogues (decrease pituitary release LH/FSH) ~50-83%
c. Colposuspension ~53-55%
d. Urethropexy / cystourethropexy 56%
e. Bulking agents
f. Transpelvic urethral sling
g. Transobturator vaginal tape
h. Artificial urethral sphincter 33-45%
- What is % continence rate for urethropexy + colposuspension?
a. 70%
- What is complication rate of urethropexy?
a. 21%
- What substance is used for a bulking agent?
a. Bovine collagen
- What is complication rate for transobturator vaginal tape?
a. 33%
- For artificial urethral sphincter, what is it made of?
a. Silicone
- What is the artificial urethral sphincter implant size based on?
a. Luminal diameter of closed cuff
- To avoid obstruction, should be __% of urethral circumference.
a. 50%
- What is complete continence rate for this surgery?
a. 36-56%
- UTI rate?
a. 63%
- What immunosuppression drug is not OK for dogs with renal transplants?
a. Tacrolimus – more severe side effects
- What method is the choice for monitoring cyclosporine concentration?
a. HPLC method – measures parent compound
- What is formulation of choice for cyclosporine and why?
a. Neoral 100 mg/ml
b. Microemulsified formulation; better GI absorption and sustained blood levels
c. More predictable
d. 1-4 mg/kg q12
- What are the goal cyclosporine target levels?
a. 300-500 ng/mL (eventually 250 ng/ml)
- What is current immunosuppressive protocol for canine kidney transplants?
a. Cyclosporine (Neoral) 2-5 mg/kg PO q12
b. Prednisolone 1 mg/kg/d PO
c. Azathioprine 3-5 mg/kg PO q48
- With the donor cat/dog (renal transplant) when is mannitol given?
a. At time of incision and 20 min before nephrectomy
- What drug for analgesia in dogs to avoid and why?
a. Morphine – concern for intussusception
- What is the kidney graft store in between procedures?
a. Ice cold phosphate-buffered sucrose organ preservation solution
- What are the former and newer anastomosis sites of renal transplantation in cats?
a. Former: External iliac a/v
b. Newer: Aorta; then CdVC
- What was the problem of former technique of renal transplantation in cats?
a. one report using this technique, ~12% of cats developed some form of pelvic limb complications, including pain, limb edema, hypothermia, paresis, or paralysis
- List 3 neoureterocystostomy techniques used
a. Intravesicular mucosal apposition
b. Extravesicular
c. Ureter + papilla excised and anastomosis with bladder (extravesicular)
- Two methods of pexy of donor kidney?
a. Allograft pexy to wall or musculoperitoneal flap (base ventral) suture to capsule
- What percent canines get intussusception post renal transplant?
a. 25%
- What is hemolytic uremic syndrome?
a. Side effect of cyclosporine therapy (cats)
b. Hemolytic anemia, thrombocytopenia, rapid deterioration of renal function secondary to glomerular & renal arteriolar platelet & fibrin thrombi
- What is mortality rate with hemolytic uremic syndrome?
a. 100%
- What is incidence of acute rejection in cats?
a. 13-26%
- What are methods of diagnosis of acute rejection?
a. AUS +/- contrast enhancement,
b. CS
c. Urine sediment stones / minerals?
- Tx for acute rejection in cats?
a. IV cyclosporine, prednisolone-Na-succinate IV, IVF
b. If no improvement evaluate for another cause
c. Newer sx? Euth?
- List complications with kidney transplants in cats vs dogs
a. Cats: acute rejection, chronic rejection, hemolytic uremic syndrome, Ca oxalate urolithiasis, retroperitoneal fibrosis, ureteral obstruction, infection, diabetes mellitus, neoplasia
b. Dogs: thromboemboli, intussusception, infection, graft rejection, renal dysfunction, cardiac failure, neurotoxicity, ocular toxicity, hepatotoxicity, gingival hyperplasia
- MST for renal transplants
a. Cats: 360-613 days
b. Dogs: 24 days (0.5-4014 d)
- Diagnostic options for ovarian remnant syndrome
a. Hormone – estradiol / progesterone
b. LH concentration
c. Anti-mullerian
d. AUS
e. CT