Uric Acid Flashcards
Uricase acts upon uric acid to form ____________ and ___________
Hydrogen peroxidase and allantoin
The H2O2 is measured quantitatively by its reaction with
3,5 dichloro-2- hydroxybenzenesulfonic acid
End product color of N-(y-antipyryl-3-chloro-5-sulfonate-p-benzoquinonemonoimine)
Red violet complex
Buffer used
Phospate buffer
Interference substance:
Hemoglobin >100mg/dl
Bilirubin >20mg/dl
Reagent stability and storage: reagent
2-8C
Reagent stability and storage: stable
2-8C
Specimen of choice
Serum or plasma (heparin and EDTA)
Urine diluted ratio:
1:10 (1+9) with distilled water
Sample stability: 2-3days
Room temp
Sample stability: 3-7days
2-8C
Sample stability: 6-12months
Frozen
Reagent blank mL
1.0 mL
Standard reagent mL
0.02 mL
Sample mL
0.02
Incubate within
37C
Incubate for how long
5mins
Incubate at room temperature within
15 mins
Read within
15mins
Wavelength used to read
520nm
Formula of uric acid in serum/plasma
Au/As x 8
Formula of uric acid in urine
Au/As x 80
Expected value for men serum/plasma:
3.4-7.0mg/dl
Expected value for women serum/plasma:
2.4-5.7 mg/dl
