Uric Acid Flashcards
1
Q
Uricase acts upon uric acid to form ____________ and ___________
A
Hydrogen peroxidase and allantoin
2
Q
The H2O2 is measured quantitatively by its reaction with
A
3,5 dichloro-2- hydroxybenzenesulfonic acid
3
Q
End product color of N-(y-antipyryl-3-chloro-5-sulfonate-p-benzoquinonemonoimine)
A
Red violet complex
4
Q
Buffer used
A
Phospate buffer
5
Q
Interference substance:
A
Hemoglobin >100mg/dl
Bilirubin >20mg/dl
6
Q
Reagent stability and storage: reagent
A
2-8C
7
Q
Reagent stability and storage: stable
A
2-8C
8
Q
Specimen of choice
A
Serum or plasma (heparin and EDTA)
9
Q
Urine diluted ratio:
A
1:10 (1+9) with distilled water
10
Q
Sample stability: 2-3days
A
Room temp
11
Q
Sample stability: 3-7days
A
2-8C
12
Q
Sample stability: 6-12months
A
Frozen
13
Q
Reagent blank mL
A
1.0 mL
14
Q
Standard reagent mL
A
0.02 mL
15
Q
Sample mL
A
0.02
