Total And Direct Bilirubin

Question 1

Most routine clinical procedures for the determination of serum bilirubin are based on

Answer 1

Classical diazo reaction of ehrlich

Question 2

first applied to the estimation of this bile pigment in serum in 1913 by

Answer 2

Van den berg and snapper

Question 3

An excellent review of many bilinubin methodologies is given by

Answer 3

Winkelman and his co-worker

Question 4

solution of bilirubin

Answer 4

Sulfanilic acid in dilute hydrochloric acid combined with sodium nitrate

Question 5

When a solution of sulfanilic acid in dilute hydrochloric acid is combined with one of sodium nitrite it formed

Answer 5

Nitrous acid

Question 6

The latter also termed

Answer 6

P-benzenediazonium sulfonate

Question 7

The latter (also termed “p-benzenediazonium sulfonate*) couples with bilirubin to produce

Answer 7

Azobilirubin

Question 8

The method utilized aqueous solution as calibrator

Answer 8

N-1-napthylethylenediamine Dihydrochloride

Question 9

The method utilized aqueous solution of N-1-napthylethylenediamaine dihydrochloride as calibrator according to

Answer 9

Bilissis and speer

Question 10

color quivalent to the labelled bilirubin level at

Answer 10

540nm

Question 11

Total bilirubin reagent

Answer 11

Sulfanilic acid in dilute hydrochloric acid

Question 12

Direct bilirubin reagent

Answer 12

Sulfanilic acid in dilute hydrochloric acid

Question 13

Bilirubin oxidants

Answer 13

Sodium nitrate

Question 14

Bilirubin calibrator

Answer 14

N-1-napthylethylenediamine-dihydrochloride

Question 15

Total Bilibubin reagent, oxidant and calibrators are

Answer 15

stable

Question 16

when stored at

Answer 16

15-30C

Question 17

Discard oxidant if a ___________ color develop

Answer 17

Dark yellow

Question 18

Specimen of choice

Answer 18

Serum or plasma (use of any common anticoagulant)

Question 19

should be free of hemolysis because

Answer 19

It can result falsely decrease

Question 20

bilirubin is highly __________.

Answer 20

photolabile

Question 21

Avoid lipemic because

Answer 21

It can cause falsely increase

Question 22

Light 30-50% be lost when expose to 1hr because

Answer 22

It can falsely decrease

Question 23

Bilirubin is stable in serum or plasma: 4-7 days

Answer 23

2-8C

Question 24

Bilirubin is stable in serum or plasma: 3months

Answer 24

20C (frozen)

Question 25

Cross hemolysis will cause falsely low bilirubin values, due to

Answer 25

inhibition of the diazo reaction by oxyhemoglo-
bin

Question 26

Total bilirubin reagent (ml/ul)

Answer 26

1.0mL (1000ul)

Question 27

Total bilirubin oxidant how many drop

Answer 27

1 drop

Question 28

Total bilirubin calibrator (ml/ul)

Answer 28

0.05ml (50uL)

Question 29

Total bilirubin sample (ml or ul)

Answer 29

0.05ml (50ul)

Question 30

Total bilirubin h2o (ml/ul)

Answer 30

0.05ml (50ul)

Question 31

Total bilirubin incubation

Answer 31

Room temp (5mins)

Question 32

Total bilirubin read at

Answer 32

540nm

Question 33

Total bilirubin calculations

Answer 33

Abs of unknown/ abs of std x 10 mg/dl

Question 34

Total bilirubin normal value

Answer 34

1.2 mg/dl

Question 35

Direct bilirubin reagent (ml/ul)

Answer 35

1.0ml (1000ul)

Question 36

Direct bilirubin oxidant how many drops

Answer 36

1 drop

Question 37

Direct bilirubin calibrator (ml/ul)

Answer 37

0.1ml (100ul)

Question 38

Direct bilirubin sample (ml/ul)

Answer 38

0.1ml (100ul)

Question 39

Direct bilirubin h2o (ml/ul)

Answer 39

0.1ml (100ul)

Question 40

Direct bilirubin incubation at

Answer 40

3mins only

Question 41

Read Direct bilirubin

Answer 41

540nm

Question 42

Direct bilirubin calculation

Answer 42

Abs of sample-abs of sb /abs of K x 10mg/dl

Question 43

Direct bilirubin calculation

Answer 43

Abs of samp-abs of sb/ abs of K x 10mg/dl

Question 44

For newborns, usually only __________ is requested.

Answer 44

total bilirubin

Question 45

Total bilirubin normal value for infant

Answer 45

12.0mg/dl

Question 46

Direct bilirubin normal value

Answer 46

0.5 mg/dl