Creatinine

Question 1

described a method for the determination of creati-nine involving a protein free filtrate and reaction with picric acid in alkaline solution.

Answer 1

Jaffe (1886)

Question 2

Although several methods have been de-scribed since, the ____________ is still most widely used.

Answer 2

classic Jaffe reaction

Question 3

This reaction is subject to interferences by a number of sub stances, including

Answer 3

Protein and glucose

Question 4

popular because they are rapid, simple and avoic
interference.

Answer 4

Kinetic procedure

Question 5

developed to combat the drawbacks.

Answer 5

Modification

Question 6

become popular because they are rapid, simple and avoic
interference.

Answer 6

Kinetic procedure

Question 7

The present method is based on a modification of the
automated reaction rate of ____________ and ____________

Answer 7

Fabinay and eringshuasen

Question 8

Method to be used fabinay and eringshausen

Answer 8

Creatinine + picric acid = creatinine picrate complex

Question 9

Method to be used jaffe

Answer 9

Protein free filtrate+ picric acid = alkaline solution

Question 10

Creatinine Acid Reagent.

Answer 10

Picric acid

Question 11

Creatinine Base Reagent,

Answer 11

Sodium hydroxide

Question 12

is a strong oxidizing agent.

Answer 12

Picric acid

Question 13

Reagent Storage and Stability: Base Reagent and Creatinine Standard are stable until the
date of their expiration when properly stored at

Answer 13

15-30C

Question 14

Reagent Storage and Stability: Working reagent is stable for 30 days when properly stored at

Answer 14

15-30C

Question 15

Do not use Working reagent if

Answer 15

Turbid

Question 16

Spectrophotometer capable of absorbance readings at,

Answer 16

510 nm

Question 17

Sample of choice

Answer 17

Serum

Question 18

Urine: Dilute a portion of well mixed and measured 24-hour urine collection _______________

Answer 18

1:20 (1+19) with distilled water.

Question 19

Sample stability: one day

Answer 19

2-8c

Question 20

Sample stability: several month

Answer 20

Frozen (-20c)

Question 21

Urine specimens must be preserved with

Answer 21

15 grams of boric acid.

Question 22

Set spectrophotometer cuvette temperature to

Answer 22

37C

Question 23

Pipet _________ of working reagent into test tubes

Answer 23

1.0 mL (1000ul)

Question 24

prewarm at

Answer 24

37°C for 3 minutes.

Question 25

Transfer ___________ of standard to test tube, mix
and immediately place in cuvette well.

Answer 25

0.050 mL (50 ul)

Question 26

Calculate change in absorbance (AA) by subtracting

Answer 26

A2-A1= Au

Question 27

Formula serum

Answer 27

Au/As x 5

Question 28

Formula urine:

Answer 28

Au/as x 5x 20

Question 29

Normal value of male serum

Answer 29

0.9-1.5 mg/dl

Question 30

Normal value male urine

Answer 30

1000-2000 mg/24hour

Question 31

Normal value female serum

Answer 31

0.7-1.4mg/dl

Question 32

Normal value of female urine

Answer 32

600-1500mg/24hrs