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Microbiology Lab 2572-02 > Unknown Cultures > Flashcards

Unknown Cultures Flashcards

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1
Q

physiological tests detect the…

A

products of enzymatic reactions

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2
Q

a “positive” test indicates that the

A

enzyme is made by the organism

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3
Q

Catalase Test

tested for:
positive test:
negative test:

A
  • catalase enzyme
  • bubbles when peroxide is added
  • no change when peroxide is added
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4
Q

Oxidase Test

tested for:
positive test:
negative test:

A
  • presence of cytochrome oxidase
  • blue color when oxidase reagent is added
  • no color change
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5
Q

what test has a time limit of 30 secs- 1 min?

A

the oxidase test

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6
Q

which medium contains sodium thioglycollate? why is this significant?

A

FTM
b/c it keeps O2 levels low

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7
Q

in an FTM tube where is oxygen absent?

A

at the bottom of the tube

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8
Q

what is used for carbohydrate fermentation?

A

4 tubes
- glucose
- lactose
- sucrose
- mannitol

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9
Q

carbohydrate fermentation produces

A

acid and/or gas

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10
Q

in carbohydrate fermentation the test tubes contain….

A
  • a durham tube
  • an indicator
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11
Q

what is the indicator found in the test tubes for carbohydrate fermentation? what color changes indicate acid and alkaline condition?

A

phenol red
- acid: yellow
- alkaline: pink

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12
Q

citrate slants contain

A
  • citrate
  • bromophenol blue indicator
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13
Q

Citrate Test

tested for:
positive test:
negative test:

A
  • citrate
  • bright blue
  • green

blue at alkaline pH
green at neutral pH

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14
Q

Startch Hydrolysis

tested for:
positive test:
negative test:

A
  • enzyme amylase
  • zone of clearing after addition of grams iodine
  • no zone of clearing
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15
Q

function of amylase

A

cleave strach into glucose

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16
Q

Hemolysis Test

tested for:
positive test:
negative test:

A
  • hemolysin
  • zone of clearing
  • no zone of clearing
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17
Q

beta-hemolysis indicates

A

complete clearing of blood around colonies

18
Q

alpha-hemolyisis indicates

A

partical clearing of blood around colonies

19
Q

gamam-hemolysis indicates

A

no hemolysis at all

20
Q

BAP is

A

TSA containing 5% sheeps blood

21
Q

Urea Hydrolysis

tested for:
positive test:
negative test:

A
  • urease
  • hot pink
  • yellow-orange

yellow-organge is the initial color so a negative result would be no color change

22
Q

Nitrate Reduction Test

tested for:
positive test:
negative test:

A
  • reduction of nitrate(NO3) to nitrite (NO2)
  • dark pink-red
  • no color change
23
Q

confirming the nitrate reduction test

tested for:
positive test:
negative test:

A
  • nitrate reduction
  • no color change after zinc addition
  • dark pink-red after zinc addition
24
Q

why does the nitrate reduction test need to be confirmed?

A

b/c some cells convert nitrite into other molecules such as N2 and NH3

25
Q

SIM stands for

A

sulfate, indole, motility

26
Q

SIM medium allows for what 3 physiological tests

A
  1. formation os H2S from catabolism of sulfur containing amino acids
  2. typrophan hydrolysis
  3. motility
27
Q

positive result for H2S production is

A

a black precipitate

28
Q

positive result for tryptophan hydrolysis is

A

pink color w/ addition of Kovac’s reagent

29
Q

positive result for molitity is when

A

the organism spreads from the line of innoculation

30
Q

what are the extra tests that differentiate the staphlococcus species?

A

MSA plate

Coagulase Test

31
Q

MSA test is selective for ____ and differentital for _________.

A
  • halophiles
  • mannitol fermentation (yellow)
32
Q

Coagulase Test causes precipitation of fibrin around

A

Staphylococcus aureus

33
Q

coagulase test indicates

A

the clotting of plasma

34
Q

What is the addition test to distinguish Streptococcues agalactiae

A

CAMP test

35
Q

In the CAMP test streptococcus agalactiae enchances the hemolysis of

A

Staphylococcus aureus

36
Q

what is a positive result for the CAMP Test

A

enhanced hemolysis

37
Q

what are the main medically important species of staphlococcus

A

S. aureus
S. epidermidis
S. saprophyticus

38
Q

what are the main medically important species of streptococcus

A
  • S. pyogenes
  • S. pneumoniae
  • S. agalactiae
39
Q

which streptococcus species is significant due to its antibiotic resistance?

A

Enterococcus faecalis

40
Q

staphylococcus and streptococcus are

A

gram positive cocci

41
Q

define pyogenic

A

pus- forming

Microbiology Lab 2572-02 (16 decks)

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