Lab 3: Pure Culture (Aseptic) Techniques Flashcards
1
Q
at what radius must we always work to prevent contamination?
A
8-10” from the bunsen burner
2
Q
how does one properly sterilize an inoculating loop?
A
by starting near the handle and working towards the loop
3
Q
in the T-streak procedure and pour plate procedures, why is the loop sterlized?
A
to reduce the # of cultures in each section of the T-streak, helps to increase the chances of having an isolated culture
4
Q
Why are agar plates inverted during incubation?
A
to minimize/prevent the accumulation of condensation which could compromise an isolated culture
