Units 5-8 Exam Flashcards
Characterized by wavelength and is measured in nanometers (nm); energy from the sun radiates to earth as waves of light; light is then absorbed by the pigments inside the chloroplast; people and blue wavelengths (shortest) contain the most energy; red (longest) have the lowest amount of energy
The electromagnetic spectrum
3 possible fates for light encountering an object
Absorbed, reflected, transmitted
light is taken up by the object is encounters and is thus no longer visible. Only this can be used in photosynthesis
Absorbed
Light is not taken up by the object, it is the color you see because it goes back to your eyes
Reflected
Light passes through an object. If you can see through an object, then light also passed through
Transmitted
Each pigment’s energy absorption is illustrated in an _______
Absorption spectrum
Photosynthetic activity can be detected by measuring oxygen production in an ______
Action spectrum
Molecules within the chloroplast that absorb energy at specific wavelengths
Pigments
Main pigment, it gives chloroplasts their green color
Chlorophyll a
Absorb light energy and pass this energy on to chlorophyll a; although each pigment has its own absorption spectrum, all the pigments work together; allow the plant to utilize the full range of the visible light spectrum
Accessory pigments
An accessory green pigment
Chlorophyll b
Method of separating major plant pigments based on differential solubility
Chromatography
Carotenoids (yellow-orange)
top
Chlorophyll a (blue-green)
middle
Chlorophyll b (yellow-green)
Bottom
What is the most soluble on TLC strips: Top, middle, or bottom?
Top
Calculation for the Rf value for each pigment on the TLC strip
Rf=a/b
Plants harness light energy and convert it into usable energy (sugar); This occurs in the chloroplasts; Plants take in CO2, water, and light; and produce sugar and O2
Photosynthesis
Thin, waxy outer layer, protects inner parts of the leaf and prevents water loss through evaporation
Cuticle
Upper and lower surfaces of leaf (contain no chloroplasts), outermost layer, like your skin
Epidermis
In upper portion of leaf interior (contains many chloroplasts and is the site of most photosynthesis)–cells are vertically oriented to increase exposure to sunlight
Palisade layer
Lower portion of leaf interior (larger air spaces allow gasses to pass freely into/out of leaf interior), contain chloroplasts
Spongy layer
Guard cells and stomata
Leaf structure
Specialized epidermal cells, regulate opening and closing of stomata via osmosis
Guard cells