Unit2 safety Flashcards
go to the notes and label the different hazard symbols
Answer on notes
Through which 2 organs or tissue can one transmit infections in the lab
Through the skin and conjuctiva (eye mucous membrane)
Which one between the eye and intact skin is an effective barrier in preventing transmission
Intact skin
What are the 7 things to do to avoid 💉 infection transmission through the eyes and skin
1.wash hands frequently
2.avoid 💦 splashing
3.avoid touching eyes
4.never apply make-up
5.dispose off waste correctly
6.prevent contact of skin with contaminated material
7.use safety goggles.
What 4 ways help us to avoid infection through the mouth
1.never mouth pipette
2.avoid touching mouth
3.dont eat, drink or smoke
4.never apply lip moisturizer
What two ways is infection transmitted through the respiratory tract
1.aerosols and 2.infective dusts
How can we avoid 1.aerosols and 2.infective dusts
- Don’t break or drop tube with broth culture
2.dont open free-fried cultures and fungal culture
3.use safe and gentle techniques
4.allow wire loop to cool
5.clean up spills immediately
6.centrifuge in sealed buckets
7.open tubed in safety cabinets.
List 10 lab facilities requirements
1.no overcrowding
2.hand basins and disposable towels
3. Safety officer
4. Separate room for records
5.disinfectants - Sodium hypochlorite
6.proper animal facilities
7.safety cabinet
8.smooth impermeable benches
9.sealed buckets for centrifuges
10.protective materials available
What is the safety code for cuts
Wash with soap and water
What is the safety code for eye
Rinse with eye bath (saline solution) for atleast 20min
What is the safety code for mouth
Spit and rinse well with water
What is the safety code for skin
Wash with disinfectant and then with soap and water
What is the safety code for clothes
Rinse with disinfectant then with soap and water, can also autoclave (sterilization method that uses high pressure steam)
What is the safety code for spills
Pour spillage with disinfectant, leave for 10min and wipe with disposable towel
What are the 3 NO’s
1.No eating 🍲 smoking 🚬 drinking 🍻
Make up💄
2.No licking 😝
3. NO fingering eyes👀
What are the 5 DO’s
1.Do wear PPE
2.Do wash hands before leaving the lab
3.Do remove laboratory before leaving lab
4.Do dispose waste correctly
5.Do wash hands after any procedure that result in possible contamination.
3 rules regarding specimens
- Discard leaking specimens
- Open respiratory pathogens containing specimen in safety cabinet
- Open containers slowly to avoid splashing and aerosols
In which 5 ways can we care for our workplace (lab)
1.disinfectants and bins should be visible
2.keep it tidy
3.keep tunes and containers in racks
4.wipe benches with disinfectant before and after a session
5.replace previous day’s jars with new ones with fresh disinfectants.
What are the 5 bench techniques
1.avoid splashing and spilling
2.discard items correctly
3.work gently
4.when flame 🔥 splatters, place loop in boiling water /disinfectant or safety cabinet
How does putting the flame in a safety cabinet help
It prevents aerosols from being breathed in.
What goes into the safety cabinet
TB and other airborne (air based) pathogens
What are the 4 rules of using a safety cabinet
- Switch off UV light and turn on ventilating fan before use.
2.correctly disinfect area inside the cabinet before and after use - After use switch on UV lamp
Lastly ensure cabinet is serviced regularly.
What are the 5rules regarding care of the workplace
1.disinfectants and bins should be visible
2.keep tubes and containers in racks
3.wipe benches with disinfectant before and after the lab
4.replace with fresh disinfectant everyday.
What are the 5 rules of bench technique
1.avoid splashing
2.discard items correctly
3.work gently
4.when flaming 🔥 are likely to splatter, put them in boiling water, disinfectant or safety cabinet.
5.no mouth pipetting
