Unit 8 Flashcards
Where is energy stored?
ATP
What does ATP do?
Provide energy for cellular activities
Cellular Respiration
reactions that break down organic monomers to release energy for ATP production
How is ATP (adenosine triphosphate) made?
by adding a phosphate group to ADP (adenosine diphosphate)
What does the formation of ATP from ADP require?
energy from food/glucose
What is ATP hydrolyzed into? (2)
- phosphate group
- ADP
What does the break down of ATP into ADP release?
Energy that can be used for cellular work
phosphorylate
add a phosphate group to a molecue
What happens when a molecule gains a phosphate group? (2)
- becomes unstable
- becomes highly reactive
What do cells use ATP for? (5)
- skeletal muscle
- cardiac muscle
- liver
- extraocular
- retinal
What are the reactants for the cellular respiration equation? (2)
- oxygen
- glucose
What are the products for the cellular respiration equation? (3)
- carbon dioxide
- water
- ATP
How are breathing and cellular respiration related? (2)
- breathing bring O2 into the body and distributes it to cells via blood
- O2 is used in mitochondria to make ATP and breathing disposes of the CO2 waste product
When are electrons moved?
when extracting energy
When are electrons gained?
when receiving energy
oxidation (2)
- loss of electrons
- loss of energy
reduction (2)
- addition of electrons
- addition of energy
What happens when glucose is oxidized? (2)
- loses high energy electrons/H atoms
- catabolic/glucose is broken down to release energy
What happens when O2 is reduced? (2)
- gains low energy electrons/H atoms
- oxygen accepts H to make water
What do high energy electron do on their way to oxygen?
donate some of their energy to generate ATP
Describe cell respiration in terms of metabolism. (2)
- It is a series of enzyme catalyzed reactions.
- It is catabolic.
Describe the conversion of ADP into ATP. (3)
- When a phosphate group is added, adenosine diphosphate becomes adenosine triphosphate.
- Adding a phosphate group is called phosphorylation.
- ATP production requires energy from food.
List three cellular processes that use ATP as a source of energy. (3)
- Muscular contractions
- Protein synthesis
- Active transport
What types of respiration can ATP be made by? (2)
- aerobic
- anaerobic
Aerobic (3)
- uses oxygen
- yields more ATP than anaerobic respiration
- four stages of reaction are used to extract energy from food to make ATP
Anaerobic (3)
- doesn’t use oxygen
- yields far less ATP than aerobic respiration
- only one reaction stage is completed
What is the aerobic respiration overall reaction?
carbohydrate + oxygen —> carbon dioxide + water + energy (38 ATPs)
What are the stages of aerobic respiration? (4)
1) glycolysis
2) link reaction
3) Kreb’s Cycle
4) Electron Transport Chain & Chemiosmosis
What do stages 1-3 aerobic respiration do? (2)
- 4 ATP
- lots of energized electrons
What does stage 4 of aerobic respiration do?
use energized electrons to make 34 ATP
Glycolysis
the splitting of sugar
Where does glycolysis occur?
cytoplasm
Electron carriers (NADH)
carry energy held by energized electrons to the electron transport chain to make ATP
What does glycolysis not require?
oxygen
What are key points about glycolysis? (3)
- first occurred before the atmosphere had oxygen
- uses enzymes to catalyze reactions
- produces ATP by substrate-level phosphorylation
State the mechanism by which glycolysis produces ATP. (1)
Substrate-level phosphorylation
Outline the process of glycolysis. (6)
1) Glycolysis occurs in the cytoplasm.
2) Starting off with glucose, that glucose is phosphorylated to become hexose bisphosphate which requires 2 ATPs.
3) Another phosphate is added to the triose phosphate to become triose biphosphate which is then oxidized causing it to lose energy/H+
4) In turn, NAD+ is reduced to NADH and 4 ADPs are reduced to 4 ATPs
5) The rest of what is left from the triose phosphates is two pyruvate molecules
6) Overall, there is a net gain of 2 ATPs.
After glycolysis is that enough?
No, there is energy left in the pyruvate
What happens after glycolysis?
If oxygen is available, pyruvate will enter the mitochondria where it will be further oxidized.
What happens in cellular respiration is oxygen is present?
reactions move to the mitochondria
matrix
contains enzymes and solutes for link reaction and Kreb’s Cycle
Inner mitochondrial membrane
site for electron transport chain and oxidative phosphorylation
Cristae (2)
- folded inner mitochondrial membrane
- meant to maximize surface area for reactions
Small inter-membrane space
more efficient generation of H+ concentration gradient
Outline the structure of a mitochondrion. (6)
- It is circular
- It has a smooth outer membrane
- Within the inner membrane is the matrix - Matrix is the inner compartment within the inner membrane
- There are ribosomes and circular DNA in the matrix
- The intermembrane space is between the outer and inner membranes.
70S Ribosomes
protein production
What is the step after glycolysis?
link reaction
What does the link reaction do?
modify pyruvate so it can enter the next stage (Kreb’s cycle)
What are the components of pyruvate?
carbons
Explain the link reaction. (6)
- The link reaction can also be known as the oxidative decarboxylation of pyruvates.
- When a pyruvate enters the link reaction, which occurs when oxygen is present, it attaches to coenzyme A.
- In the process, it is oxidized and decarboxylated.
- Where a carboxyl group, CO2 is lost, which becomes a waste product of the link reaction.
- It also loses electrons/ H atoms/ energy via oxidation which goes to NAD+ which is reduced to NADH.
- The result from this reaction after happening 2 times is 2 NADH and 2 Acetyl CoA, which was left of the pyruvate after the CO2 was lost. These things go onto the next stage, the Krebs cycle.
decarboxylation
removal of carboxyl group and releasing it as CO2
Where does the link reaction occur?
matrix of the mitochondria
What is another name for the link reaction?
oxidative decarboxylation
What is oxidative decarboxylated in the link reaction?
pyruvate
How is pyruvate oxidative decarboxylated? (2)
decarboxylated - because CO2 is lost
oxidized - because it loses H atoms/e-/energy
What is the waste from the link reaction?
2CO2
What is the net yield from the link reaction? (2)
- 2 acetyl CoA per glucose molecule (because there are two pyruvates to go through the link reaction)
- 2NADH/2H+
What is stage 3 of cellular respiration?
the Krebs Cycle
When does the Kreb’s Cycle occur?
when oxygen is present
Where does acetyl CoA come from?
the link reaction
What is acetyl CoA used for in the Krebs Cycle?
to make citric acid
What happens to citric acid in the Krebs Cycle? (2)
- it is broken down in the Krebs cycle to release energy
- energy is transferred to electron carriers and used to make ATP directly
Where does the Krebs Cycle occur?
the matrix
What are the steps of the Krebs Cycle? (8)
- acetyl CoA brings its carbons to oxaloacetate
- citric acid is formed
- the coenzyme goes back to the link reaction
- citric acid goes through oxidative decarboxylation
- citric acid is oxidized
- NAD+ is reduced to NADH H+
- CO2 is waste product using oxygen - 5 carbon is result
- 5 carbon goes through oxidative decarboxylation (same result as citric acid)
- 4 carbon goes goes through oxidative decarboxylation
- ADP –> ATP through substrate level phosphorylation
- FAD reduced to FADH2
- NAD+ reduced to NADH H+ - returns back to oxaloacetate
How many carbons does acetyl CoA have?
2
How many carbons does oxaloacetate have?
4
How many carbons does citric acid have?
6
What does the Kreb’s cycle produce for 1 glucose? (3)
- 2 ATP
- 6 NADH, 2 FADH2 (electron carriers)
- 4 CO2
What does substrate-level phosphorylation do in the Kreb’s Cycle?
produce ATP
Indicate two places where decarboxylation occurs. (1)
Going from citric acid to C5 and going from C5 to oxalacetate
What does oxidative decarboxylation make in the Kreb’s cycle? (3)
make:
- NADH
- FADH2
- CO2
Which releases more energy to the cell? Glycolysis or Krebs cycle?
Krebs cycle
Why does the Krebs cycle produce more energy? (2)
- it produces a lot of NADH and FADH2
- most of the energy from glucose is still in electrons at the end of the Kreb’s cycle
Where do electron carriers take electrons?
the ETC
electron transport chain
removes energized electrons from carriers and uses energy to build a concentration gradient
chemiosmosis
uses concentration gradient to make ATP
What does ETC + chemiosmosis equal?
oxidative phosphorylation
oxidative phosphorylation
using energy from oxidation of electron carriers to make ATP